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A method for determining the fingerprint of Hirudo phenantha medicinal material

A technology of fingerprints and determination methods, which is applied in the field of determination of fingerprints of the medicinal material Hirudo phenatus

Active Publication Date: 2015-10-21
HEBEI YILING MEDICINE INST
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  • Abstract
  • Description
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Problems solved by technology

[0002] Philippine leech (Poecilobdella manillensis), commonly known as Phnom Penh leech, belongs to the order of Hirudidae, Hirudaceae, and Hirudogenus. It is mainly distributed in Guangxi, Guangdong, Hainan, Fujian and other provinces and regions, and can be artificially cultivated. It has Significant anticoagulant, antithrombotic, thrombolytic, and antiplatelet aggregation activities, the main chemical components are fatty acids, amino acids and proteins, among which the reported active components are hirudin P6, hirudin P18, phenanthrene A and phenanthrene Hirudin B, the acute toxicity test shows that Hirudinus phenanthrene has no obvious toxic and side effects, so it has significant medicinal value. So far, neither the Pharmacopoeia nor the local standard has recorded it, nor has there been any report on the fingerprint

Method used

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  • A method for determining the fingerprint of Hirudo phenantha medicinal material
  • A method for determining the fingerprint of Hirudo phenantha medicinal material
  • A method for determining the fingerprint of Hirudo phenantha medicinal material

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Experimental program
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Embodiment 1

[0049] The preparation method of the test solution: crush the dry product of Hirudo phenanthae, pass through a 40-mesh sieve, take 0.40g, add 5ml of deionized water for ultrasonication for 15min, centrifuge at 4500r min-1 for 15min, filter with a 0.45μm filter membrane, and the filtrate is used as a high-efficiency Liquid chromatography need testing solution standby;

[0050] Chromatographic conditions: The chromatographic column is a C18 column, the specification is 250mm×4.6mm, 5μm; the mobile phase: the A phase is acetonitrile, the B phase is water, gradient elution; the elution program is 0→5min, A is 0%, 5→ 23min, A is 0%→4%, 23→24min, A is 4%→5%, 24-66min, A is 5%→21.4%; flow rate: 1ml min -1 ; Column temperature: 20°C; detection wavelength is 254nm.

[0051] Take the test solution, inject samples according to the above-mentioned chromatographic conditions, record the chromatogram, and compare it with the standard chromatogram, and it is completely fitted.

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Abstract

The invention provides a method for measuring a finger-print of a poecilobdella manillensis drug. Poecilobdella manillensis (poecilobdella manillensis) is commonly referred to as a phnom penh leech, belongs to arhynchobdellida, hirudinidae and poecilobdella, has the obvious functions of resisting coagulation, resisting thrombus, thrombolysis, and antiplatelet aggregative activity, has significant medical value, has not recorded in pharmacopoeia or other local standards so far, and has no any report about the finger-print. According to the method disclosed by the invention, the poecilobdella manillensis drug is measured by a high-performance liquid chromatography; the finger-print of the poecilobdella manillensis drug is built; a method is provided for entire quality evaluation of the drug, and the method is used for quality control of the poecilobdella manillensis drug material.

Description

technical field [0001] The invention relates to a method for determining the fingerprint of Hirudo phenanthae medicinal material, which belongs to the technical field of drug analysis. Background technique [0002] Philippine leech (Poecilobdella manillensis), commonly known as Phnom Penh leech, belongs to the order of Hirudidae, Hirudaceae, and Hirudogenus. It is mainly distributed in Guangxi, Guangdong, Hainan, Fujian and other provinces and regions, and can be artificially cultivated. It has Significant anticoagulant, antithrombotic, thrombolytic, and antiplatelet aggregation activities, the main chemical components are fatty acids, amino acids and proteins, among which the reported active components are hirudin P6, hirudin P18, phenanthrene A and phenanthrene Hirudin B, the acute toxicity test shows that Hirudinus phenanthrene has no obvious toxic and side effects, so it has significant medicinal value. So far, it has not been recorded in the Pharmacopoeia and local stan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 孟作环马振嗣乔莉李文烈贾继明宋剑
Owner HEBEI YILING MEDICINE INST
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