Fluorescence enhanced microarray biochip based on micro/nano periodic structures and method for preparing same

Abstract

The invention relates to a fluorescence enhanced microarray biochip based on micro / nano periodic structures and a method for preparing the same. According to the chip, the micro / nano periodic structures based on the surface plasmon resonance enhanced fluorescence technology serve as a substrate material of the microarray biochip; a series of micro / nano periodic structures are processed on a glass or / and polymer substrate, wherein the period is 400-800 nm, and the depth is 20-100 nm; and multiple layers of functional films are deposited on the glass or / and polymer substrate to ensure that the glass or / and polymer substrate has the function of surface plasmon resonance coupling enhanced fluorescence. The fluorescence signal strength and sensitivity of biomolecule microarray analysis on a substrate are improved by about 10 times, so that the micro / nano periodic structures have the potential to become a brand new low-cost high-throughput and ultrasensitive microarray biochip substrate material. The fluorescence enhanced microarray biochip based on the micro / nano periodic structures has the characteristics that surface plasmon resonance enhanced fluorescence is combined with the microarray biochip technology, the microarray biochip is simple in structure, flexible and various in materials and suitable for mass production and high-throughput detection.

Description

technical field [0001] The present invention relates to a novel micro / nano periodical structure based on surface plasmon resonance (Surface Plasmon Resonance, SPR) enhanced fluorescence, which can be used as the base material of biosensing chip, and the protein microarray can be written and scanned on the base. Increasing the fluorescence signal intensity on the surface by about 10 times helps to improve the sensitivity of detecting target molecules, making this micro / nano periodic structure a new low-cost, high-throughput, ultra-sensitive microarray biochip substrate material. Background technique [0002] Microarray biochip (Microarray) is a high-throughput analysis method widely used in genes, proteins, carbohydrates, cells and other biological components. Its main principle is to use mechanical or chemical methods to prepare a matrix arrangement of various biomolecules including DNA, carbohydrates, proteins, etc. on an activated solid substrate, and then analyze various ...

AI Technical Summary

Problems solved by technology

In terms of surface modification of the chip, the amination surface was first developed and utilized, but the electrostatic interaction between it and biomolecules will cause more non-specific adsorption, resulting in a strong background; It is easy to be oxidized by oxygen into carboxyl groups and partially inactivate the aldehyde groups, which is not conducive to the long-term preservation of the chip; recently, more and more researchers use epoxy to modify the surface, which can fix biomolecules with hydroxyl and amino groups at the end, And the epoxidized surface can be stored stably in the air for several months

In the selection of chip biomarker materials, the traditional radioactive labeling method has high sensitivity, but its operation is complicated and harmful, which is now uncommon; the use of metal nanoparticles as biomarker materials has good photostability, but The experimental process is cumbersome; fluorescent labeling is currently the most commonly used labeling form, which has the characteristics of high sensitivity and easy operation, but the photostability of fluorescent labels is generally poor, and the photobleaching effect is serious. During the experiment, care should be taken to avoid light. Prevent photobleaching from happening

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