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Method for preparing phospholipid nano/micron tube by using finger-shaped micro-electrode

A micro-electrode and micro-tube technology, applied in the field of preparing phospholipid tubes using finger-shaped micro-electrodes, to achieve the effects of less environmental pollution, expanded preparation range, and high yield

Inactive Publication Date: 2014-04-09
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the use of electric field to make phospholipids spontaneously form tubular structures

Method used

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  • Method for preparing phospholipid nano/micron tube by using finger-shaped micro-electrode
  • Method for preparing phospholipid nano/micron tube by using finger-shaped micro-electrode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1) Electrode preparation and cleaning:

[0015] The prepared finger-shaped microelectrodes (with a width of 50-200 μm for fingers) were ultrasonically cleaned with absolute ethanol and distilled water for 5-15 minutes, then dried with nitrogen, and treated with a plasma cleaner for 20-30 seconds before use.

[0016] 2) Applying and drying of phospholipids:

[0017] Dissolve phospholipids in chloroform (99.95%) to prepare a 1-20 mg / mL phospholipid solution, take 1-10 μL of the phospholipid solution on the finger-shaped microelectrode, and apply it back and forth with a syringe needle 1-6 times to make it evenly Lay it on the finger-shaped microelectrode, and then put it into a vacuum desiccator to dry it under vacuum for 2~3 hours to completely remove the residual chloroform solvent.

[0018] 3) Assembly of the preparation device:

[0019] Place the dried phospholipid-coated planar electrode between the glass slide and the cover glass to form a figure 1 In the reactio...

Embodiment 2

[0023] 1) Electrode preparation and cleaning:

[0024] ① Finger electrodes can be prepared as follows:

[0025] (1) ITO surface cleaning: Ultrasonic cleaning of ITO conductive glass with ethanol and distilled water for 15 minutes, N 2 Blow dry, process with plasma cleaning machine for 30s, put on heating plate to preheat at 110℃ for 10min.

[0026] (2) Set the plastic sealing machine (GMP Photonex-Sync235) to preheat at 110°C, press the AM175 photoresist on the ITO substrate to form a film, place the finger electrode mask, expose and develop it with soft ultraviolet (365nm) for 2s, 65°C Heating and curing for 4 minutes, with a mass percentage of 1.5% Na 2 CO 3 The solution was washed after ultrasonication for 7s, and a finger-shaped electrode pattern was preliminarily obtained.

[0027] (3) AUTOLAB PGSTAT302N electrochemical workstation was used to selectively corrode finger electrodes by chronoamperometry. The hydrochloric acid solution with a volume ratio of 1:5 was use...

Embodiment 3

[0038] 1) Electrode preparation and cleaning:

[0039] The prepared 100 μm finger microelectrodes were ultrasonically cleaned with absolute ethanol and distilled water for 15 minutes, then dried with nitrogen, and treated with a plasma cleaner for 20 seconds before use.

[0040] 2) Applying and drying of phospholipids:

[0041] Dissolve phospholipids in chloroform to prepare a 5 mg / mL phospholipid solution. Take 10 μL of the phospholipid solution on the finger-shaped microelectrode, and use a syringe needle to spread it back and forth three times to spread it evenly on the finger-shaped micro-electrode. It was placed in a vacuum desiccator and vacuum-dried for 3 hours to completely remove residual chloroform solvent.

[0042] 3) Assembly of the preparation device:

[0043] 30 μL of glucose (150 mM) solution was injected between the dried flat electrode coated with phospholipid and the cover glass to form a reaction device, and the solution height range was 100 μm.

[0044] ...

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Abstract

The invention provides a method for preparing a phospholipid nano / micron tube by using a finger-shaped micro-electrode and relates to a preparation method of a phospholipid tube. A plane finger-shaped micro-electrode is utilized and an electric forming method is used for realizing the preparation of the phospholipid tube in a certain solution height range, a certain alternating-current voltage range, a certain frequency range and a certain reaction time range; the shape of the phospholipid tube is good and the yield is higher; and the method can be widely applied to relative biological membrane properties of the fields including cell biology, membrane biophysics, biochemistry, bionics and the like, and can be applied to researches and application of cell simulation, medical carriers, target feeding and the like. The method disclosed by the invention fills in the blank of the preparation method of the phospholipid tube, enlarges the preparation range in a breakthrough manner, and has the advantages of small electrode area, low energy consumption, fast growing speed, short preparation period, simplicity in operation, moderate reaction conditions, small pollution to the environment, good controllability of the length and the diameter of the phospholipid tube and the like.

Description

technical field [0001] The invention relates to a method for preparing a phospholipid tube, in particular to a method for preparing a phospholipid tube by using a finger microelectrode. Background technique [0002] As an important state of phospholipids, phospholipid tubes are a place for material exchange between cells, and have important applications in the fields of biology, medicine, and analytical chemistry. At present, the preparation methods of phospholipid tubes mainly include microfluidic method, vesicle extrusion method, and solvent changing method. At present, there is no report about making phospholipids spontaneously generate tubular structures under the action of an electric field. Contents of the invention [0003] The purpose of the present invention is to provide a method for preparing phospholipid nano / micro tubes by using finger microelectrodes. The controllable preparation of phospholipid tubes further enriches and expands the preparation methods of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J19/08
Inventor 韩晓军毕洪梅
Owner HARBIN INST OF TECH
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