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Method for high-sensitivity detection of silver ions through circular dichroism

A circular two-spectrum, sensitive detection technology, applied in the field of analytical chemistry, to achieve the effect of improving sensitivity and simplifying reaction conditions

Active Publication Date: 2013-01-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is still blank to use this assembly system combined with the principle of nucleic acid mismatch recognition to construct heavy metal silver ion detection sensors.

Method used

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  • Method for high-sensitivity detection of silver ions through circular dichroism
  • Method for high-sensitivity detection of silver ions through circular dichroism
  • Method for high-sensitivity detection of silver ions through circular dichroism

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Embodiment 1

[0048] (1) Synthesis of 10nm gold nanoparticles

[0049] The synthesis scheme of 10nm gold nanoparticles is as follows: add 60mL ultrapure water and 1mL 1% chloroauric acid solution to a clean three-neck flask as solution A; take a clean small bottle, add 4mL 1% trisodium citrate solution, 0.1mL 1% tannic acid solution, 0.1mL 50mM potassium carbonate solution and 16mL ultrapure water were used as liquid B; both liquids A and B were heated to 60°C, then liquid B was quickly added to liquid A under high-speed stirring, and the final reaction liquid was Stirring was continued at 60° C. for 30 min to form a deep red solution, then the solution was heated to reflux for 10 min to form a bright red solution, and finally cooled to room temperature to form citric acid-stabilized 10 nm gold nanoparticles.

[0050] (2) Synthesis of 25nm gold nanoparticles

[0051] The synthesis scheme of 25nm gold nanoparticles is as follows: add 47.5mL ultrapure water to a clean three-neck flask, add 0...

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Abstract

The invention discloses a method for high-sensitivity detection of silver ions through circular dichroism, belonging to the technical field of analytic chemistry. The method comprises the following steps: respectively coupling gold nanoparticles with two different grain sizes and two specific nucleic acids rich in a cytosine (C) sequence to obtain AuNPs10nm-DNA1 and AuNPs25nm-DNA2, adding silver ions to cause AuNPs10nm-DNA1 and AuNPs25nm-DNA2 to generate C-Ag<+>-C mispairing identification, reducing through trisodium citrate, reacting AuNPs10nm-DNA1, AuNPs25nm-DNA2 and silver ions under the liquid environment to form a binary assembly so as to form plasma chirality, and causing the gold nanoparticles to generate a circular dichroism CD signal in a visible region. The CD signal intensity in the liquid environment is measured through the circular dichroism so as to measure the content of the silver ions. According to the invention, reaction is only performed in the liquid environment, a step of cleaning is unnecessary, one-step reaction is only required, the reaction condition is simplified, the sensitivity of detection is improved, and the leading level in the world is achieved.

Description

technical field [0001] The invention discloses a method for highly sensitive detection of silver ions by using circular dichroism spectrum, which belongs to the technical field of analytical chemistry. Background technique [0002] Currently commonly used heavy metal detection methods are based on physical or chemical methods, such as inductively coupled plasma emission spectrometry, flow injection atomic absorption spectrophotometer, inductively coupled plasma mass spectrometry, polarography and voltammetric electrodes, atomic fluorescence spectroscopy Analysis, Atomic Furnace Absorption Spectroscopy. The biggest advantage of the above-mentioned main instrument detection methods is that they are accurate, but these traditional methods require complicated pre-treatment, long analysis time, high labor intensity and high cost, and can only be performed by professionals in the laboratory, requiring professional It is difficult for technicians to carry out a large number of sam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/19
Inventor 胥传来许宙王利兵匡华徐丽广马伟刘丽强
Owner JIANGNAN UNIV
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