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High-value utilization and separation method for oil producing microorganism energy microalgae

A technology for oil-producing microorganisms and separation methods, which is applied in the field of high-value utilization and separation of oil-producing microorganisms and microalgae, which can solve the problems of inability to fully utilize pigments and proteins, poor separation effects, and high costs, and achieve reduced enzymatic action time , increase cell permeability, good oil quality

Inactive Publication Date: 2014-08-20
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention designs a method for high-value utilization and separation of oil-producing microbial energy microalgae, which solves the technical problem that the existing oil-producing microbial energy microalgae separation methods cannot fully obtain the polysaccharides, oils, pigments and proteins contained in them. Utilization, and the separation effect is not good, the cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: Select Chlorella for use

[0030] Centrifuge the chlorella fermentation liquid at 4000rpm / min for 10min, remove the supernatant to obtain the wet chlorella cells, weigh 1.0g of the wet chlorella cells into a 100ml Erlenmeyer flask, add 25ml of deionized water, and heat at 90°C 1.5h, cooled, and centrifuged to obtain supernatant A and precipitate A. Weigh 0.065g cellulase, 0.035g neutral protease, cellulase activity is 1200U / g, neutral protease activity is 60000U / g, add citric acid-sodium citrate buffer solution with pH=5, pH= 5 is the pH reaction system selected for the optimal enzyme action in the experiment. Set the volume to 100ml, take 25ml of the enzyme solution and add it to the chlorella wet cell precipitation A, the hydrolysis temperature is 42°C, stir for 4h, and inactivate the enzyme at 90°C for 10min. Centrifuge the hydrolyzed suspension at 4000r / min for 10min to obtain supernatant B and precipitate B, combine supernatant A and supernatant B t...

Embodiment 2

[0033] Embodiment 2: select Scenedesmus for use

[0034] Centrifuge the Scenedesmus fermentation liquid at 4000rpm / min for 10min, remove the supernatant to obtain Scenedesmus cells, weigh 1.0g of Scenedesmus wet cells into a 100ml Erlenmeyer flask, add 25ml of deionized water, heat at 90°C for 1.5h, cool, Centrifuge to obtain supernatant A and precipitate A. Weigh 0.075g cellulase, 0.025g alkaline protease, cellulase activity is 1200U / g, alkaline protease activity is 2×105U / g, add citric acid-sodium citrate buffer solution with pH=4 to set To 100ml, take 25ml of enzyme solution and add it to Scenedesmus wet cell precipitation A, hydrolyze at 40°C, stir for 4h, inactivate the enzyme at 90°C for 10min, and centrifuge the hydrolyzed suspension at 4000r / min for 10min to obtain Supernatant B and pellet B. Afterwards step is with embodiment 1.

[0035] The final product, the polysaccharide extraction rate was 78.14%. The measured pigment removal rate was 90.92%, and the polysacc...

Embodiment 3

[0036] Embodiment 3: select spirulina for use

[0037] Centrifuge the spirulina fermentation broth at 4000rpm / min for 10min, remove the supernatant to obtain spirochetes, weigh 0.8g of the wet spirochetes in a 100ml Erlenmeyer flask, add 25ml of deionized water, heat at 90°C for 1.5h, cool, and centrifuge to obtain Supernatant A and pellet A. Weigh 0.067g neutral protease, the activity of alkaline protease is 60000U / g, add citric acid-sodium citrate buffer solution with pH=7 to set the volume to 100ml, take 20ml enzyme solution and add it to the wet cells of Scenedesmus sp. The temperature was 42°C, stirred for 6h, and the enzyme was extinguished at 90°C for 10min. The hydrolyzed suspension was centrifuged at 4000r / min for 10min to obtain supernatant B and precipitate B. Afterwards step is with embodiment 1.

[0038] The final product has a polysaccharide extraction rate of 75.74%. The measured pigment removal rate was 91.22%, and the polysaccharide retention rate was 89.7...

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Abstract

The invention relates to a high-value utilization and separation method for oil-producing microorganism energy microalgae. The method comprises the following steps of: 1, sequentially treating collected microalga wet thalli by using hot water and centrifuging to obtain supernate A and precipitates A; 2, sequentially adding enzyme into the precipitates A and centrifuging to obtain supernate B and precipitates B; 3, mixing the supernate A and the supernate B and concentrating, adding cold ethanol at 4 DEG C, standing for more than 12 hours, and filtering mixture to obtain crude polysaccharide precipitates; and 4, adding water into the crude polysaccharide precipitates obtained in the step 3, dissolving, deproteinizing, separating to obtain protein precipitates and a deproteinized polysaccharide solution, performing freeze drying on protein precipitates or drying the protein precipitates to obtain crude protein products, decolorizing the deproteinized polysaccharide solution to obtain refined polysaccharide, and treating the precipitates B for multiple times to obtain uranidin, chlorophyllide, high-protein feed and grease.

Description

technical field [0001] The invention relates to the field of microbial engineering, in particular to a method for high-value utilization and separation of oil-producing microorganisms and microalgae. Background technique [0002] With the depletion of petrochemical resources and the increasing awareness of environmental protection, the development of clean and renewable biodiesel is particularly urgent. Energy microalgae has become one of the important sources due to its wide variety, wide distribution, fast reproduction and low cost. In addition, its fatty acid composition is similar to that of plant-derived edible oils, and can replace animal and vegetable oils as edible oils, and even as health-care functional oils. In addition to a large amount of lipids, microalgae are also rich in active ingredients such as polysaccharides, pigments, proteins and essential amino acids. It has the effects of enhancing immunity, anti-cancer, anti-radiation damage, anti-aging, and lower...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/405C08B37/00C09B61/00A23K1/14C11B1/04C11B1/10C11B1/00
Inventor 张栩段美蓉谭天伟
Owner BEIJING UNIV OF CHEM TECH
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