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Biological preparation for deodorization and chromium reduction and preparation method thereof

A technology of compound microbial inoculants and preparations, applied in the field of environmental biology, can solve problems such as secondary pollution of chromium-containing sludge, and achieve the effects of controlling the spread of diseases, eliminating odor, and promoting cell division.

Inactive Publication Date: 2013-01-16
TIANJIN BOMAN BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, biosorbents are mostly prepared by cultivating activated sludge with complex strains, which is prone to secondary pollution of chromium-containing sludge

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A compound microbial preparation, comprising the following components:

[0030] (1) a composite microbial bacterial agent accounting for 10% of the total weight, said composite microbial bacterial agent is composed of sporogenous yeast, Bacillus sphaericus, Lactobacillus plantarum and Rhodobacter sphaeroides;

[0031] (2) Microbe carrier accounting for 90% of the total weight, wherein, 50 parts of bran or rice bran, 200 parts of organic bentonite, and 25 parts of water are mixed in parts by weight.

[0032] The preparation method of the composite microbial bacterial agent is as follows:

[0033] (1) Amplified culture of bacteria

[0034]Sporobolomyces roseus culture medium: glucose 20g, peptone 5g, yeast extract powder 2g, MgSO 4 0.005g, distilled water 1000mL, pH 5.5-6.0. Cultivate at 28°C for 24-72h.

[0035] LB medium for culturing Bacillus sphaericus: 1% peptone, 0.5% yeast extract, 1% sodium chloride, pH 7.0. Incubate at 37°C for 24-48h.

[0036] MRS medium ...

Embodiment 2

[0050] Embodiment 2 is basically the same as Example 1

[0051] The preparation method of composite microbial preparation is as follows:

[0052] (1) Amplified culture of bacteria

[0053] Put the spore-throwing yeast into the liquid medium after being activated on the slant, place it on a shaker at 200r / min, and cultivate it at 28°C for 72 hours; Cultivate under low temperature for 24 hours; activate Lactobacillus plantarum on a slant and insert it into a liquid medium, and cultivate it at 37°C for 48 hours; activate Rhodobacter sphaeroides on a slant and insert it into a liquid medium, and place it in a 3000lux light incubator at 30°C Conditioned for 48h. Each bacterial culture medium is with embodiment 1.

[0054] Detect the number of bacteria of each microorganism to ensure that the number of viable bacteria of each bacteria is ≥1×10 8 / mL, it is mixed by parts by weight: 150 parts of spore-throwing yeast, 80 parts of Bacillus sphaericus, 120 parts of Lactobacillus pla...

Embodiment 3

[0058] Embodiment 3 is basically the same as Example 1

[0059] The preparation method of composite microbial preparation is as follows:

[0060] (1) Amplified culture of bacteria

[0061] Put the spore-throwing yeast into the liquid medium after being activated on the slant, place it on a shaker at 200r / min, and cultivate it at 28°C for 72h; Cultivate under the same conditions for 24 hours; activate the Lactobacillus plantarum on a slant and insert it into the liquid culture medium, and cultivate it at 37°C for 24 hours; activate the Rhodobacter sphaeroides on the slant and insert it into the liquid culture medium, at 30°C, 3000lux light Cultured in the incubator for 72h. Each bacterial culture medium is with embodiment 1.

[0062] Detect the number of bacteria of each microorganism to ensure that the number of viable bacteria of each bacteria is ≥1×10 8 / mL, it is mixed by parts by weight: 200 parts of spore-throwing yeast, 40 parts of Bacillus sphaericus, 80 parts of La...

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PUM

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Abstract

The invention discloses an environment-friendly composite microorganism preparation and a preparation method thereof, and belongs to the technical field of environmental biology; the composite microorganism preparation of the invention comprises the following components: (1) a composite microbial agent accounting for 10% of the total weight and comprising sporobolomyces, bacillus sphaericus, lactobacillus plantarum and rhodobacter sphaeroides; (2) a microorganism carrier accounting for 90% of the total weight and comprising 20-50 parts of wheat bran or rice bran, 160-300 parts of organic bentonite, and 40-80 parts of water. The invention compounds bacteria and fungi, enlarges the application scope, and is applicable to not only deodorization treatment of municipal solid household garbage, production sewage, and the like, but also degradation of waste water polluted by heavy metal chromium.

Description

technical field [0001] The invention relates to an environment-friendly compound microbial preparation, which belongs to the field of environmental biotechnology and can be used for deodorization treatment of urban solid domestic garbage, production sewage, etc., and degradation of waste water polluted by heavy metal chromium. Background technique [0002] With the rapid development of social economy and the continuous advancement of urbanization, the problem of indoor and outdoor environmental pollution is becoming more and more serious, especially the problem of odor. my country's environmental pollution and odorous gases mainly come from urban landfills and transfer stations, sewage treatment plants, livestock and poultry farms and other places. Due to various reasons (such as the discharge of feces, soapy water, and some kitchen organic sewage and other substances) in family bathrooms and kitchen sewers, odors can also be emitted. In addition to causing unpleasant feeli...

Claims

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Application Information

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IPC IPC(8): A61L11/00A61L9/01C02F3/34A61L101/52
Inventor 林萍黄亮
Owner TIANJIN BOMAN BIOLOGICAL TECH CO LTD
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