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Acinetobacter calcoaceticus TA221 strain producing homoserine lactone and application thereof

A technology of homoserine lactone and TA221, which is applied in the fields of application, bacteria, and animal repellents, and can solve problems such as blanks in the development of new biological control products

Inactive Publication Date: 2013-01-09
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a certain gap between us and foreign countries in terms of the dosage form and quality of microbial pesticides, especially in the field of new biological control product development.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: the production of Acinetobacter calcoaceticus TA221 bacterial agent of the present invention:

[0015] 1. Medium preparation:

[0016] 1) Strain preservation medium: peptone peptone 10g, yeast extract powder 5g, sodium chloride 5g, agar 20g, add water to 1L, pH7.0-7.5;

[0017] 2) Fermentation medium: peptone peptone 10g, yeast extract powder 5g, sodium chloride 5g, agar 20g, add water to 1L, pH7.0-7.5;

[0018] All the above media were sterilized at 121°C for 15-30min;

[0019] 2. Strain activation: Pick the strain CGMCC No.4895 to the strain preservation medium, streak it several times, and culture it overnight at 30°C; pick a single colony and inoculate it in the fermentation medium, and culture it at 30°C, 200r / min for 12-15h ;

[0020] 3. Bacterial agent preparation: inoculate the fermentation medium with the strain liquid activated in step 2 according to the inoculum amount of 10%, and culture it with aeration at 30°C for 12-20h to obtain a live b...

Embodiment 2

[0021] Embodiment 2: the prevention and treatment of tobacco wildfire disease by Acinetobacter calcium acetate TA221:

[0022] Acinetobacter calcoaceticus TA221 was prepared as a bacterial suspension (100-fold diluted microbial agent) and sprayed on tobacco leaves and dried naturally. According to the routine plant pathogenic bacteria inoculation method, different concentrations of Pseudomonas syringae pv.tabaci were inoculated, and each treatment was replicated three times, and the incidence was recorded after inoculation. The experimental results showed that 3 days after the inoculation of Pseudomonas syringae, when the inoculation dose was 10 5 CFU / mL concentration already caused strong lesions in the control tobacco. And for the experimental group tobacco sprayed with Acinetobacter calcoacetate TA221, when the inoculation amount of Pseudomonas syringae was 10 7 Tobacco showed weak lesions at CFU / mL concentration. The experimental results showed that the control effect o...

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PUM

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Abstract

The invention relates to acinetobacter calcoaceticus TA221 separated from an advanced plant. The strain can be used for producing homoserine lactone and controlling plant diseases, and belongs to the field of biotechnology. As proved by identification, the strain is an acinetobacter calcoaceticus strain, and is collected in the China General Microbiological Culture Collection Center with the collection number CGMCC No.4895. The strain can be well colonized on the surface of a plant in an application process, shows high stability and high continuity of control effects, and has a very good application prospect on the control of plant diseases.

Description

Technical field: [0001] The invention relates to the field of biotechnology, and relates to a strain of Acinetobacter calcoacetate TA221 capable of producing homoserine lactone isolated from tobacco leaves, and its application in the field of biological control. Background technique: [0002] The practice of using a large number of chemical pesticides and other means to prevent plant diseases in modern agricultural production has caused serious environmental pollution, and the resulting ecological risks are enough to attract people's attention. The high pesticide residues in food and vegetables have seriously affected human health. In addition, the non-point source pollution of a large amount of residual pesticides has caused a serious chain reaction, and the ecological environment pollution caused by it cannot be underestimated. The sustainable control and pollution-free management of pests have attracted increasing attention and become an active research field, laying the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00A01G13/00C12R1/01
Inventor 马安周吕迪邓雪梅高婕庄国强
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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