Method and system of laser scanning phase-microscope imaging
A microscopic imaging and laser scanning technology, applied in the field of optical imaging, can solve the problems of fusion, no sample phase information transformation, unfavorable laser scanning confocal microscopy technology fusion, etc., to shield the influence of imaging quality, and the switching process is simple. Easy, high-contrast imaging results
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[0017] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.
[0018] Such as figure 1As shown, the laser scanning phase microscopy imaging system of the present invention includes a fluorescence microscope 1, a laser confocal scanning system 2 and a photoelectric detection system 3; the fluorescence microscope 1 includes an objective lens 11, a tube mirror 12 and a scanning mirror 13; The focusing scanning system 2 includes a laser 21, a dichroic mirror 22, an X-direction scanning galvanometer 23, a Y-direction scanning galvanometer 24, a filter 25, a converging lens 26, and a pinhole 27; At the light entrance hole of the pinhole 27, the photodetection system 3 is located in the light exit direction of the pinhole 27, and is used to receive the fluorescence transmitted by the biological sample. The laser 21 emits excitation light to the dichroic mirror 22 for reflection or transmission (light splitting), and th...
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