Preparation and application of Au@Pd core-shell material constructed lung cancer tumor marker immunosensor

A technology of tumor markers and immunosensors, which is applied in the field of preparation of lung cancer tumor markers immunosensors with gold-palladium core-shell materials, which can solve the problems of many factors in the detection results of chemiluminescence analysis, reduce the sensitivity and reproducibility of the method, and the luminescence of samples cannot be solved. Reproducibility and other problems to achieve the effects of avoiding inactivation problems, easy integration, and high reproducibility

Inactive Publication Date: 2012-12-12
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] (1) Radioimmunoassay: This method has been widely used in various fields of biomedicine. It has the advantages of simple operation and low cost, but its serious radioactive pollution and low sensitivity limit its application.
[0008] (2) Enzyme immunoassay assay method: This method has the characteristics of simple preparation, long validity period, and no pollution to the environment, and has been rapidly popularized and developed. However, the signal time is short due to the easy inactivation of the enzyme, which reduces the efficiency of the method. Sensitivity and reproducibility
[0009] (3) Chemiluminescence immunoassay assay: This method has the advantages of high sensitivity, strong selectivity, good reproducibility, and easy operation. After a chemical reaction, the luminescence of the sample cannot be reproduced

Method used

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  • Preparation and application of Au@Pd core-shell material constructed lung cancer tumor marker immunosensor
  • Preparation and application of Au@Pd core-shell material constructed lung cancer tumor marker immunosensor
  • Preparation and application of Au@Pd core-shell material constructed lung cancer tumor marker immunosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Preparation method of electrochemical immunosensor for lung cancer tumor markers

[0054] (1) Preparation of AuPd core-shell nanomaterials, the steps are as follows:

[0055] 1) Preparation of colloidal solution of gold nanoparticles: add 0.010g HAuCl to a three-neck flask 4 ·3H 2 0, add 100mL ultrapure water, magnetically stir, add the mass fraction of 3mL when boiling and be 1% sodium citrate, keep stirring, the solution becomes wine red, keep boiling for 40min, until the color of the gold nanoparticle colloidal solution no longer changes , after the reaction is complete, cool to room temperature.

[0056] 2) Preparation of chloropalladium acid solution: take 0.0638g PdCl 2 , add 0.6mL of 1.2mol·L -1 HCl, add ultrapure water to dilute to 3mL, sonicate until completely dissolved.

[0057] 3) Preparation of AuPd core-shell nanomaterials: Take 60 mL gold nanoparticle colloidal solution, put it in a water bath at 4 °C, stir and add 1.5 mL H 2 PdCl 4 so...

Embodiment 2

[0067] Example 2 Preparation method of electrochemical immunosensor for lung cancer tumor markers

[0068] (1) Preparation of AuPd core-shell nanomaterials, the steps are as follows:

[0069] 1) Preparation of colloidal solution of gold nanoparticles: add 0.008g HAuCl to a three-neck flask 4 ·3H 2 0, then add 90mL ultrapure water, carry out magnetic stirring, add the mass fraction of 2.5mL when boiling is 1% sodium citrate, keep stirring, the solution becomes wine red, and keep boiling for 45min, until gold nanoparticle colloidal solution The color does not change any more, and after the reaction is completed, cool to room temperature.

[0070] 2) Preparation of chloropalladium acid solution: take 0.0638g PdCl 2 , add 0.5mL of 1.2mol·L -1 HCl, add ultrapure water to dilute to 3mL, and then sonicate until completely dissolved.

[0071] 3) Preparation of AuPd core-shell nanomaterials: Take 55mL gold nanoparticle colloidal solution, place it in a 4°C water bath, add 1.0mL...

Embodiment 3

[0081] Example 3 Preparation method of electrochemical immunosensor for lung cancer tumor markers

[0082] (1) Preparation of AuPd core-shell nanomaterials, the steps are as follows:

[0083] 1) Preparation of colloidal solution of gold nanoparticles: add 0.012g HAuCl to a three-neck flask 4 ·3H 2 0, then add 110 mL of ultrapure water, carry out magnetic stirring, and add 3.5 mL of mass fraction of 1% sodium citrate when boiling, keep stirring, the solution becomes wine red, and keep boiling for 50 min until the gold nanoparticles colloid The color of the solution did not change any more, and after the reaction was completed, it was cooled to room temperature.

[0084] 2) Preparation of chloropalladium acid solution: take 0.0638g PdCl 2 , add 0.7mL of 1.2mol·L -1 HCl, add ultrapure water to dilute to 3mL, and then sonicate until completely dissolved.

[0085] 3) Preparation of AuPd core-shell nanomaterials: Take 65mL gold nanoparticle colloidal solution, place it in a ...

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Abstract

Belonging to the technical field of novel functional materials, biosensing and clinical examination, the invention relates to preparation and application of an Au@Pd core-shell material constructed lung cancer tumor marker immunosensor. The method of the invention makes use of the characteristics of a large specific surface area, good biocompatibility, high catalytic efficiency and the like of anAu@Pd core-shell nano-material, and substantially improves the sensitivity of the immunosensor. The method adopts the Au@Pd core-shell nano-material to immobilize a first antigen and mark a second antigen, and by means of layer-by-layer self-assembly, a lung cancer tumor marker electrochemical immunosensor can be constructed. The Au@Pd core-shell material constructed lung cancer tumor marker immunosensor provided in the invention has the advantages of high sensitivity, good specificity, as well as easy operation, and can realize sensitive, rapid, and accurate detection of a variety of lung cancer tumor markers in a serum sample, thus having important significance for early diagnosis of lung cancer.

Description

technical field [0001] The invention relates to the preparation and application of a gold-palladium core-shell material to construct a lung cancer tumor marker immunosensor. Specifically, it is a labeled electrochemical immunosensor for detecting multiple lung cancer tumor markers constructed by using AuPd core-shell nanomaterials, which belongs to the technical fields of new functional materials, biosensing and clinical testing. Background technique [0002] Cancer is one of the diseases that seriously threaten human health, with high morbidity and high mortality. If cancer is diagnosed in the early stage of cancer development, the survival rate of patients will be significantly improved. [0003] Therefore, early tumor diagnosis has become a hot research issue [see: (a) Rükan G.; Deirdre M.; Alex F.; Mayreli O.; Ciara K. O'Sullivan. Anal. Chem., 2011, 83 ( 2), 563-570. (b) Zhong Z.Y.; Peng N.; Qing Y.; Shan J.L.; Li M.G.; Guan W.; Dai N.; 5629. (c) Zhang H.S.; Qi S.W. Cl...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N27/327
Inventor 魏琴吴丹郭爱平张勇李贺杜斌马洪敏于海琴庞雪辉朱宝存崔振涛王志玲闫良国罗川南孙蒙范大伟
Owner UNIV OF JINAN
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