Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing mononuclear cell pre-transplantation state

A preparation method and technology of nuclear cells, applied in the field of preparation of mononuclear cells before transplantation, can solve the problems of lack of uniform standards, inconsistent preparation and storage states, etc., and achieve the effect of standardized packaging, easy collection, and fewer processing steps

Inactive Publication Date: 2012-12-12
张宁坤 +1
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the existing mononuclear cell collection and preparation methods are for the purpose of experiments, and the preparation methods and final preparations before use are various. When conducting research for the purpose of transplantation, each research unit conducts research on umbilical cord blood mononuclear cells before transplantation. The preparation and storage state of the umbilical cord blood mononuclear cells are inconsistent, and there is no uniform standard at present, so the problem that needs to be solved now is the preparation method of umbilical cord blood mononuclear cells before transplantation and what kind of state they are in after preparation to best meet the needs of cell transplantation , in order to achieve the most ideal effect is a method we are looking for

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021]Example 1: Aseptically collect the discarded cord blood of healthy people in an anticoagulant plasma collection bag, and transport it to the cell treatment room within 1 hour at a temperature of 2 degrees Celsius. Place the centrifuge tube in a low-speed centrifuge at a speed of 1500 rpm for 15 minutes. At this time, the cord blood in the centrifuge tube is divided into two layers: the upper layer is the plasma layer, and the lower layer is the cells. Gently transfer the upper plasma layer into a new centrifuge tube for later use, then add normal saline to the centrifuge tube containing the remaining cells, the amount of normal saline added is 6 times the volume of the remaining cells, mix well to form a cell suspension , the number of centrifuge tubes can be increased to distribute the excess liquid; add 25 ml of lymphocyte separation medium with a density of 1.077 to a new 50 ml centrifuge tube, and then slowly pour 25 ml of cell suspension along the wall of the centrif...

Embodiment 2

[0022] Example 2: Aseptically collect the discarded cord blood of healthy people in an anticoagulant plasma collection bag, and transport it to the cell treatment room within 2 hours at a temperature of 4 degrees Celsius. Place the centrifuge tube in a low-speed centrifuge at 2000 rpm for 12 minutes. At this time, the cord blood in the centrifuge tube is divided into two layers: the upper layer is the plasma layer, and the lower layer is the cells. Gently transfer the upper plasma layer into a new centrifuge tube for later use, then add normal saline to the centrifuge tube containing the remaining cells, the amount of normal saline added is 5 times the volume of the remaining cells, mix well to form a cell suspension , the number of centrifuge tubes can be increased to distribute the excess liquid; add 25 ml of lymphocyte separation medium with a density of 1.077 to a new 50 ml centrifuge tube, and then slowly pour 25 ml of cell suspension along the wall of the centrifuge tube ...

Embodiment 3

[0023] Example 3: Aseptically collect the discarded cord blood of healthy people in an anticoagulant plasma collection bag, and transport it to the cell treatment room within 3 hours at a temperature of 6 degrees Celsius. Put the centrifuge tube into a 50ml centrifuge tube and centrifuge it in a low-speed centrifuge at 2500 rpm for 13 minutes. At this time, the cord blood in the centrifuge tube is divided into two layers: the upper layer is the plasma layer and the lower layer is the cells. Gently transfer the upper plasma layer into a new centrifuge tube for later use, then add normal saline to the centrifuge tube containing the remaining cells, the amount of normal saline added is 4 times the volume of the remaining cells, mix well to form a cell suspension , the number of centrifuge tubes can be increased to distribute the excess liquid; add 25 ml of lymphocyte separation medium with a density of 1.077 to a new 50 ml centrifuge tube, and then slowly pour 25 ml of cell suspen...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing a mononuclear cell pre-transplantation state. Waste umbilical cord blood of a healthy person is collected in a bacteria-free mode, blood plasma is separated, normal saline is used for diluting a cellular layer, a density gradient separation method is used for separating a mononuclear cell, using separated umbilical cord blood plasma to perform dilution and heavy suspension and after the mononuclear cell is washed, the concentration of the mononuclear cell is adjusted, and the mononuclear cell is sealed in a blood plasma bag and in a state before application. The method is different from existing methods in processing program, reduces the types, the number and the time of reagents contacted with the mononuclear cell, does not need the steps of cultivation, passage, cryopreservation and the like, and avoids the influence of other reagents contacted with the mononuclear cell to survival and growth of the mononuclear cell. The method uses the umbilical cord blood plasma to obtain the umbilical cord blood mononuclear cell which is subjected to final dilution, heavy suspension and washing, enables the umbilical cord blood mononuclear cell to be in a growing-environment state before collection and application, enables extracorporeal time of the umbilical cord blood mononuclear cell to shortened by 1.5 hours, has great promoting effect on vitality, survival and growth of the cell and meets the application requirement for various inspections before the application.

Description

technical field [0001] The invention relates to a method for preparing mononuclear cells before transplantation, in particular to a method for preparing umbilical cord blood mononuclear cells before cell transplantation. Background technique [0002] The treatment and prevention of diseases by cell transplantation has become an emerging discipline and the frontier science of medical development. Recent studies have shown that cell transplantation can not only treat difficult diseases such as cardiovascular diseases, blood diseases, endocrine diseases, and neurological diseases, but also play a role Disease prevention and health effects. There are many types and sources of cells, including adult cells and embryonic cells, bone marrow and peripheral blood mononuclear cells, mesenchymal stem cells, adipocytes, umbilical cord mesenchymal stem cells, in vitro induced stem cells, umbilical cord blood mesenchymal stem cells and mononuclear stem cells Cells, which provide an ideal ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/0786
Inventor 张宁坤高连如李田昌
Owner 张宁坤
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com