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Microorganism culture detection device in oxygen-controllable environment and detection method using same

A microbial cultivation and detection device technology, applied in the field of microbial cultivation and detection devices, can solve the problems of high cost, complex detection equipment, cumbersome operation, etc., and achieve the effects of high accuracy, more detection items, and expanded detection range

Inactive Publication Date: 2012-12-12
嘉兴杰赛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method is a very important means in microbiological testing, but it usually requires more complex testing equipment. In order to control the oxygen environment in the culture conditions, the operation is often cumbersome and the cost is high.

Method used

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  • Microorganism culture detection device in oxygen-controllable environment and detection method using same
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  • Microorganism culture detection device in oxygen-controllable environment and detection method using same

Examples

Experimental program
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Effect test

Embodiment 1

[0059] Example 1: To detect NH produced during the growth of obligate aerobic bacteria (Pseudomonas) 3 For example, the combination of detection reagent, oxygen generator and diaphragm is as follows: the detection reagent added in the reaction chamber 21 of the detection cover 2 can be bromocresol purple semi-solid gel or phenol red gel; the diaphragm placed in the detection cover 2 3 is a parchment paper semipermeable membrane; the oxygen generating agent added in the cavity 412, such as calcium peroxide and the like. Before culturing, the culture bottle 1 and the detection cap 2 are assembled and connected. After culturing under specific conditions for a certain period of time, the mixed gas generated by the biochemical reaction enters the reaction chamber 21 of the detection cap 2 through the diaphragm 3, and the reaction of the detection reagent is observed. If bromocresol If the purple semi-solid gel is purple, and the phenol red gel is red, the detected substance contain...

Embodiment 2

[0060] Example 2: Taking the detection of obligate anaerobic bacteria (oral Bacteroides) as an example to produce esculetin using aescin, the combination of detection reagent, oxygen scavenger and diaphragm is: deoxygenation added to the culture solution of culture bottle 1 The reagent is cysteine ​​or sodium thioglycolate, etc.; the detection reagent added in the reaction chamber 21 of the detection cover 2 is ferric citrate solution; Vinyl fluoride (PVDF) filter membrane; Add an oxygen scavenger in the cavity 412, can be: sodium borohydride, or sodium bicarbonate etc.; Before cultivating, culture bottle 1 and detection cap 2 are assembled and connected, and cultivated for a certain period of time under specific conditions Finally, turn the device upside down so that the ions produced by the liquid culture medium containing the sample to be tested pass through the diaphragm 3 and enter the reaction chamber 21 of the detection cover 2, and observe the reaction phenomenon of the...

Embodiment 3

[0061] Example 3: To detect the production of H during the growth of facultative anaerobic bacteria (enterococcus) 2 S is an example, the combination of detection reagent, oxygen generator / scavenger, and diaphragm is as follows: the oxygen scavenger added to the culture solution of the culture bottle 1 is cysteine, and the detection reagent added to the reaction chamber 21 of the detection cover 2 is lead acetate paper strips, or lead acetate semi-solid gel; the diaphragm 3 placed in the test area 2 is a polytetrafluoroethylene filter membrane, parchment semi-permeable membrane; before or after culture, the culture bottle 1 and the detection cap 2 are assembled and connected, and the After cultivating under certain conditions for a certain period of time, H 2 S enters the reaction chamber 21 of the detection cover 2 through the diaphragm 3, and observes the reaction phenomenon of the detection reagent. If the lead acetate paper strip or the lead acetate semi-solid gel is black...

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Abstract

The invention relates to a microorganism culture detection device in an oxygen-controllable environment and a detection method using the same, belonging to the technical field of microorganism culture. The microorganism culture detection device comprises a culture bottle (1), a detection cover (2), a diaphragm (3) and a diaphragm fixing device (4), wherein the middle part of the detection cover (2) is provided with a hollow reaction cavity (21) protruded upwards; the center of the diaphragm fixing device (4) is provided with a through hole (41) and a groove (411); the front surface of the diaphragm fixing device (4) is provided with a concave die cavity (412) and a ventilation port; a detection reagent is placed in the reaction cavity (21) of the detection cover (2); an oxygen eliminating agent or oxygen generating agent is placed in the die cavity (412); a sample to be detected is inoculated with a liquid culture medium in the culture bottle (1); and the culture bottle (1) is in threaded connection with the detection cover (2) in a separated manner. The invention has the advantages that the structure is simple, the components are convenient to replace and the oxygen-controllable environment is easy to realize, and is suitable for industrial production, storage and transportation. Besides, the invention has the characteristics of flexible operation, quick and sensitive detection and high accuracy.

Description

technical field [0001] The invention relates to a microorganism cultivation and detection device with a controllable oxygen environment and a detection method thereof, belonging to the technical field of microorganism cultivation equipment. Background technique [0002] The detection of microbial species, genetic variation, and biological phenotype status is an indispensable routine inspection item in daily work in the fields of medical care, inspection and quarantine, food safety, and disease control. [0003] At present, the commonly used detection methods of bacterial biological phenotype state include: observing the morphological characteristics of bacteria, culture conditions and biochemical reactions, etc. For example, detecting the physical and chemical characteristics of metabolites such as acids and gases produced by microorganisms under certain culture conditions, such as different oxygen-containing environments, can be used as the basis for determining the species...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12Q1/04
CPCC12M41/34C12M23/08C12M23/38
Inventor 王海晶黄洋杨扬赵万千
Owner 嘉兴杰赛生物科技有限公司
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