Flavonoid metabolic profiling analysis method based on nanofluidic channel liquid chromatogram/mass spectrum

A technology of metabolic profiling and liquid chromatography, applied in the direction of analyzing materials, material separation, measuring devices, etc., to achieve the effect of ensuring stability, ensuring stability, and simple pretreatment process

Active Publication Date: 2013-12-04
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, most of the literature reports on the analysis of flavonoids in soybeans are limited to the determination of certain flavonoid metabolites or the total flavonoid content after hydrolysis, and only a few reports on profile analysis also require secondary extraction and purification processes

Method used

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  • Flavonoid metabolic profiling analysis method based on nanofluidic channel liquid chromatogram/mass spectrum
  • Flavonoid metabolic profiling analysis method based on nanofluidic channel liquid chromatogram/mass spectrum
  • Flavonoid metabolic profiling analysis method based on nanofluidic channel liquid chromatogram/mass spectrum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Soybean Seed Sample Collection

[0025] The five soybean varieties are: Jiyu 73, Changnong 16, Jiunong 30, Hedou 12, and Zhonghuang 13. Among them, Jiyu 73, Changnong 16 and Jiunong 30 are planted in Jilin. Hedou 12 and Zhonghuang 13 were planted in Jinan. Soybean seed samples were stored in a -80°C refrigerator after collection.

[0026] 2. Analysis method

[0027] 2.1 Soybean seed sample pretreatment

[0028] Soybean seed samples were taken out from the -80°C freezer and waited in a desiccator for their temperature to return to room temperature. Each soybean seed sample was pulverized in a pulverizer and passed through a 60-mesh sieve to obtain a powder with uniform particle size. Divide soybean seed powder into plastic tubes and set aside.

[0029] Weigh 3 parts (0.1 g each) of soybean seed samples of 5 varieties and place them in 5 mL glass tubes respectively.

[0030] Weigh 0.5 g of each soybean seed sample, mix well, and use it as a quality control (QC) ...

Embodiment 2

[0051] In order to further illustrate the characteristics of the method described in this patent, the applicant compared different sample pretreatment methods and chips with different enrichment capacities.

[0052] 1) The soybean sample was extracted and purified by SPE method to improve the response of low-concentration compounds. The difference from Example 1 is that the Oasis HLBSPE column was used to pre-enrich and purify the soybean sample according to the standard procedure after the soybean sample was sonicated for 20 minutes. , the flow rate is about 10ml / min, and then washed with 1ml of water and 0.05ml of methanol, the target extract is finally washed with 0.5ml of methanol and 0.5ml of methanol / dichloromethane (1:1), and the collected solution is lyophilized and rinsed with 1ml of water Reconstitution, sample loading analysis, all analysis conditions and parameters are the same as in Example 1. It was found that after the second extraction, the response of most co...

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Abstract

The invention discloses a nanofluidic channel liquid chromatogram / mass spectrum method of soybean flavonoid metabolic profiling analysis. By the adoption of a simple sample pretreatment method, under the conditions of high enrichment capacity of channels and optimized segregation analysis, profiling analysis of 73 flavonoids can be realized by minimal sample size. The analysis method provided by the invention is simple and rapid, has advantages of small sample amount and good repeatability, and is suitable for batch analysis of real samples.

Description

technical field [0001] The invention relates to the field of analytical chemistry, and relates to a method for analyzing soybean flavonoid metabolism profiles based on chip nanoflow liquid chromatography / mass spectrometry (LC-Chip / MS). Background technique [0002] An important direction of the development of modern analytical chemistry is to develop new methods of micro-scale and integrated separation and analysis. In order to improve the sensitivity and throughput of analysis, reduce the cost of analysis and sample consumption, the development of micro-scale and integrated analysis methods has become the frontier of current research. Nanoflow liquid chromatography technology is a technology developed in the 1990s. Because of its advantages of low sample consumption, fast analysis speed and easy integration, it has attracted extensive attention from researchers and has become a new technology in the field of miniaturized analysis. Research hotspots. The recently emerging ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/08
Inventor 赵春霞常玉玮吴泽明路鑫周佳许国旺
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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