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Method for extracting tylosin by tylosin fermentation broth

A technology of tylosin and fermentation liquid, which is applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problems that cannot be used to produce tilmicosin and tylosine, and achieve the production process Safe and reliable, raw material cost reduction, and the effect of reducing the generation of impurities

Active Publication Date: 2012-10-24
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the Tylosine produced by most enterprises cannot meet the requirements of the Chinese Veterinary Pharmacopoeia, so it cannot be used to produce tilmicosin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Pretreatment: Tylosin fermentation broth volume 10m 3 , the titer is 13368u / ml, and the total billion is 133.7. The temperature of the fermentation broth is controlled at 20-22°C, and 1m of 20-22% polyaluminum sulfate solution is added 3 , adjust the pH of the fermented liquid to be 1.2, continue to stir for 30 min, let stand for 50 min, and filter the fermented liquid with a plate and frame filter to obtain a tylosin filtrate of 3.1 m 3 .

[0042] Extraction: use organic solvent butyl acetate to extract tylosin solution, the volume of butyl acetate is 1.1m 3 . A centrifugal separator is used for secondary countercurrent extraction. During the extraction process, the temperature is controlled at 40-42° C., the pH of the primary extraction is controlled at 7.0-10.0, and the pH of the secondary extraction is controlled at 8.0-11.0. After the extraction is finished, the raffinate titer is 287u / ml, and the volume of butyl acetate is 1.1m 3 .

[0043] Stripping: add pu...

Embodiment 2

[0050] Pretreatment: Tylosin fermentation broth volume 10m 3 , the titer is 13513u / ml, and the total billion is 135.1. The temperature of the fermentation broth is controlled at 25-26°C, and 1.5m of 24-25%% polyaluminum sulfate solution is added 3 , adjust the pH of the fermented liquid to be 3.5, continue to stir for 30min, let stand for 55min, filter the fermented liquid with a plate and frame filter, and obtain a tylosin filtrate of 3.3m 3 .

[0051] Extraction: use organic solvent butyl acetate to extract tylosin solution, the volume of butyl acetate is 1.3m 3 . A centrifugal separator is used for secondary countercurrent extraction. During the extraction process, the temperature is controlled at 44-46° C., the pH of the primary extraction is controlled at 7.0-10.0, and the pH of the secondary extraction is controlled at 8.0-11.0. After extraction, the titer of raffinate is 271u / ml, and the volume of butyl acetate is 1.2m 3 .

[0052] Stripping: add purified water 8....

Embodiment 3

[0059] Pretreatment: Tylosin fermentation broth volume 10m 3 , the titer is 13316u / ml, and the total billion is 133.2. The temperature of the fermentation broth is controlled at 28-30°C, and 29-30% of polyaluminum sulfate solution is added to 2m 3 , adjust the pH of the fermented liquid to be 5.8, continue to stir for 30 min, let stand for 60 min, filter the fermented liquid with a plate and frame filter, and obtain tylosin filtrate 3.2m 3 .

[0060] Extraction: use organic solvent butyl acetate to extract tylosin solution, the volume of butyl acetate is 1.4m 3 . A centrifugal separator is used for secondary countercurrent extraction. During the extraction process, the temperature is controlled at 48-50°C, the pH of the primary extraction is controlled at 7.0-10.0, and the pH of the secondary extraction is controlled at 8.0-11.0. After the extraction is finished, the titer of the raffinate is 246u / ml, and the volume of butyl acetate is 1.4m 3 .

[0061] Stripping: add pu...

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Abstract

A method for extracting tylosin by tylosin fermentation broth is characterized by including steps of firstly pretreating the tylosin fermentation broth to obtain tylosin filtrate, then subjecting the tylosin filtrate to extracting, reextracting, decolorizing filtering, crystallizing, ageing, rinsing and vacuum drying to obtain the tylosin. The method is used for directly extracting the tylosin by the tylosin fermentation broth serving as raw materials, tylosin solubility of intermediates of the tylosin accords with quality standards of Chinese Veterinary Pharmacopoeia. The integral method is mild in production conditions, simple to operate, safe and reliable in preparation process and low in loss of organic solvent and has the advantages of energy conservation, consumption reduction, low production cost, high work efficiency and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedicine extraction, in particular to a method for extracting tylosine from tylosin fermentation liquid. Background technique [0002] Tylosine, also known as Tylosin and Tylosin, is a macrolide antibiotic obtained in the United States in 1959 from the culture medium of Streptomyces freundii. Tylosine is a white plate-like crystal, insoluble in water and alkaline. The products include tartrate, phosphate, hydrochloride, sulfate and lactate, which are easily soluble in water. [0003] As an intermediate of tylosin series products, tylosine plays a very important role. Tylosine raw materials at home and abroad are mainly used in three aspects, one is to make the raw materials into injections after aseptic treatment; one is to make them into salt antibiotic products as downstream products; the other is to use them directly Tylosine raw materials synthesize tilmicosin through a series of chemical reacti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/08C07H1/08
Inventor 任勇王文超奇乃李小萍
Owner 宁夏泰瑞制药股份有限公司
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