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New use of 6-(benzylamino)-2-[[1-(hydroxymethyl)propyl]amino]-9-isopropylpurine

A technology of benzylamino and propyl purine, which is applied in the direction of antiviral agents, active ingredients of heterocyclic compounds, etc., to achieve the effect of inhibiting infection replication, important social benefits and economic benefits

Inactive Publication Date: 2012-10-24
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no report about the anti-RNA virus activity of 6-(benzylamino)-2-[[1-(hydroxymethyl)propyl]amino]-9-isopropylpurine, nor about the inhibition of Cell cycle-dependent kinases have been reported to inhibit the replication of RNA viruses, let alone 6-(benzylamino)-2(R)-[[1-(hydroxymethyl)propyl]amino]-9-isopropylpurine The report of anti-EV71 virus infection caused by hand, foot and mouth disease

Method used

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  • New use of 6-(benzylamino)-2-[[1-(hydroxymethyl)propyl]amino]-9-isopropylpurine
  • New use of 6-(benzylamino)-2-[[1-(hydroxymethyl)propyl]amino]-9-isopropylpurine
  • New use of 6-(benzylamino)-2-[[1-(hydroxymethyl)propyl]amino]-9-isopropylpurine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] This example mainly illustrates that 6-(benzylamino)-2-[[1-(hydroxymethyl)propyl]amino]-9-isopropylpurine is anti-EV71 virus infection in RD cells (human rhabdomyosarcoma cells) Disease activity.

[0020] Materials and Methods

[0021] 1. RD cell, virus and drug configuration

[0022] The EV71 virus strain is the international standard strain BrCr, which is amplified and propagated by infecting RD cells, then mixed, aliquoted and stored at -70°C for later use. The RD cell culture medium is DMEM (GIBCO) culture medium containing 10% fetal bovine serum. A maintenance solution containing 2% fetal bovine serum was used for the determination of virus infection and cytopathic changes. 6-(Benzylamino)-2-[[1-(hydroxymethyl)propyl]amino]-9-isopropylpurine was dissolved in DMSO to 20mM, and then stored at -20℃ for later use.

[0023] 2.6-(Benzylamino)-2-[[1-(hydroxymethyl)propyl]amino]-9-isopropylpurine inhibits the dose-dependent effect of EV71 virus-induced cytopathic effects

[0024...

Embodiment 2

[0035] This example mainly shows that in the EV71 infection mouse model, 6-(benzylamino)-2-[[1-(hydroxymethyl)propyl]amino]-9-isopropylpurine dose-dependently increases EV71 virus infection The survival rate of mice improved the slow weight gain of suckling mice caused by EV71 virus infection, and significantly inhibited the RNA level of EV71 virus in the mouse lung, brain, intestine and muscle.

[0036] Materials and Methods

[0037] 1. ICR suckling mice, viruses and control drugs

[0038] The animal model is SPF grade 7-day-old ICR suckling mice, and the EV71 virus strain is the international standard strain BrCr strain. The positive control drug is IFNα-2a.

[0039] 2.6-(Benzylamino)-2-[[1-(hydroxymethyl)propyl]amino]-9-isopropylpurine anti-EV71 virus activity detection in vivo

[0040] Order 6 litters of SPF grade 7-day-old ICR suckling mice, weigh and group them before the start of the experiment. Mice were set up 6-(benzylamino)-2-[[1-(hydroxymethyl)propyl]amino]-9-isopropylpur...

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Abstract

The present invention relates to a new use of a small molecule inhibitor, and specifically to a use of a CDK small molecule selective inhibitor 6-(benzylamino)-2-[[1-(hydroxymethyl)propyl]amino]-9-isopropylpurine or a pharmaceutically-acceptable salt thereof in preparation of drugs for treatment of hand-foot-mouth disease virus infection-related diseases caused by EV71 and the like.

Description

Technical field: [0001] The invention relates to a new use of the chemical drug 6-(benzylamino)-2-[[1-(hydroxymethyl)propyl]amino]-9-isopropylpurine, specifically a cyclin dependent Cyclin-dependent kinases (CDKs) selective inhibitor 6-(benzylamino)-2-[[1-(hydroxymethyl)propyl]amino]-9-isopropylpurine in the preparation of treatment and prevention EV71 (Human enterovirus 71) virus (Human enterovirus 71) infection of new use in medicine. Background technique [0002] Enterovirus 71 (EV71) is a member of the Enterovirus genus of the Picomaviridae family (Picomaradae), and belongs to human enterovirus A. EV71 can cause a large-scale outbreak, which can be accompanied by severe CNS complications or fatal pulmonary edema, and its infection hazards are very serious. The current anti-EV71 virus treatment drugs are only broad-spectrum antiviral drugs such as ribavirin, but the antiviral effect is average. Therefore, research and development of effective methods to prevent and control ...

Claims

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Application Information

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IPC IPC(8): A61K31/52A61P31/14
Inventor 王升启杨静刘娟李康韩明明
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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