Method for managing experimental animal respiration

Abstract

The invention provides a method for managing experimental animal respiration, which utilizes a clinic anesthesia machine for the anesthetization of the animal experiment, performs tracheal cannula on an animal, adopts an animal temperate maintenance instrument to keep warm, and keeps the body temperature at the physical level (37 plus or minus 0.5 DEG C) all the time during the anesthesia process. The method is used for solving the animal respiration management problem during the traditional anesthesia process, the same inhalation anesthesia concentration and the stable body environment are also maintained during the whole anesthesia process. By the method, not only is the stress response of the experimental animal reduced, but also the vital sign stability of the experimental animal can be maintained during the inhalation anesthesia process of a long time (such as 4 hours), the mortality is low and the reviving can be stable, and the influences of trauma stress, hypoxia-ischemia, hypercapnia, acidosis and other interference factors on the experimental result can be eliminated. The method effectively controls the respiratory tract of the experimental animal, and also maintains the inhalation anesthesia of the volatile anesthetics with constant concentration, which is output to the animal.

Description

technical field [0001] The invention belongs to the field of experimental methods, and relates to an experimental animal respiratory management method, in particular to a method for maintaining anesthesia while controlling the respiratory tract of experimental animals. Background technique [0002] In the process of animal experiments, the animals generally need to be anesthetized before the operation of the animals, and the anesthetics generally used in the laboratory, such as chloral hydrate and pentobarbital, have different degrees of inhibition on the respiration and circulation of experimental animals The effect is manifested as a drop in blood pressure, a slowdown in heart rate, a decrease in tidal volume, and a slowdown in respiratory rate. In some experiments with special requirements, it is necessary to manage the animal's respiratory tract, and this is generally done with an animal ventilator, but the ventilator lacks the function of an anesthesia machine, and it c...

AI Technical Summary

Problems solved by technology

The above shows that this method of anesthesia, especially for young animals shortly after birth, can easily cause respiratory depression in such an environment of inhalational anesthetics and lead to metabolic disorders such as respiratory acidosis. The exposure model of inhalational anesthetics used in the research field of "influence" cannot rule out the influence of internal environment disturbance on neurodevelopment, and it is also completely different from the inhalational anesthesia methods used clinically today!

[0003] In summary, the stress response caused by tracheotomy and the respiratory acidosis caused by respiratory depression cannot be affected by the above two methods on neurodevelopment. Excluded, there are still some defects in the above

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