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A kind of antioxidant peptide derived from hickory chick and its preparation method and application

An anti-oxidative peptide, morel technology, applied in the preparation method of peptide, chemical instruments and methods, application, etc., can solve the problem of lack of neuroprotective agents, and achieve the effect of slowing down the damage of oxidative stress on the nervous system

Active Publication Date: 2021-04-20
SAAS BIOTECH & NUCLEAR TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on the above analysis, the prevention and treatment of HIBD requires excellent neuroprotective agents, but such neuroprotective agents are relatively lacking at present

Method used

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  • A kind of antioxidant peptide derived from hickory chick and its preparation method and application
  • A kind of antioxidant peptide derived from hickory chick and its preparation method and application
  • A kind of antioxidant peptide derived from hickory chick and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] An antioxidant peptide derived from morel, the sequence of the antioxidant peptide is:

[0051] Ala-Tyr-Asp-Gln-Ile-Asp-Ala-Ala-Pro-Glu-Glu-Lys.

[0052] The antioxidant peptide consists of 12 amino acids with a relative molecular mass of 1348Da.

Embodiment 2

[0054] Isolation, purification and sequence identification of antioxidant peptides derived from morel:

[0055] Weigh 100 g of Morchella importuna dry fruiting body, pulverize with a traditional Chinese medicinal material grinder, and pass through a 40-mesh sieve to obtain Morchella importuna dry fruiting body powder.

[0056] Configure phosphate buffered saline buffer (PBS buffer): Potassium dihydrogen phosphate (KH 2 PO 4 ) 0.27g, disodium hydrogen phosphate (Na 2 HPO 4 ) 1.42g, sodium chloride (NaCl) 8g, potassium chloride (KCl) 0.2g, add about 800mL of deionized water and stir to dissolve, then add concentrated hydrochloric acid to adjust the pH to 7.4, and finally dilute to 1L.

[0057] Add samples according to the ratio of solid to liquid of Morchella dry product / PBS buffer solution 1:20. After adding the sample, stir overnight with a magnetic stirrer at 120 rpm, and centrifuge at 5000 r / min to obtain the supernatant. Collect the supernatant, select a dialysis bag w...

Embodiment 3

[0063] Solid Phase Synthesis of Antioxidant Peptides from Morchella

[0064] Through LC-MS, we determined that the target polypeptide is composed of 12 amino acids and has a relative molecular mass of 1348Da. Now we are carrying out solid-phase synthesis to obtain morel-derived antioxidant peptides more efficiently.

[0065] Reagent materials required for solid-phase synthesis: 2-CL resin, amino acid (hereinafter expressed as Fmoc-aa-oh), dimethylformamide (DMF), dichloromethane (DCM), methanol, N,N'-diiso Propylcarbodiimide (DIC), N,N-Diisopropylethylamine (DIEA), 1-Hydroxybenzotriazole (HOBT), Trifluoroacetic Acid (TFA), Triisopropylsilane (TIS) , pure water, EDT, ether

[0066] Methods as below:

[0067] 1. Weigh 2g of 2-CL resin, put it into the reactor, soak it in DCM for 10min, wash it twice with DMF, then wash it once with DCM, and set it aside.

[0068] 2. Weigh the first (starting from the C-end) Fmoc-aa-oh (2g*0.3mmol / g*1.33=0.798 mmol), dissolve it with 5mL DCM, ...

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Abstract

The invention belongs to the field of biological small molecule active peptides, and relates to an antioxidant peptide derived from morel fruit body protein, specifically an antioxidant peptide derived from morel and its preparation method and application. The antioxidant peptide comprises the following sequence: Ala-Tyr-Asp-Gln-Ile-Asp-Ala-Ala-Pro-Glu-Glu-Lys. The antioxidant peptide consists of 12 amino acids with a relative molecular mass of 1348Da. The invention isolates the natural anti-oxidation polypeptide from the morel fruit body, verifies and explains the mechanism of the polypeptide for preventing and treating ischemic-hypoxic brain injury. The obtained active polypeptide is an excellent neuroprotective agent, has the function of slowing down the damage of the nervous system caused by oxidative stress, and can be used in the preparation of anti-ischemic-hypoxic brain injury medicines or health care products.

Description

technical field [0001] The invention belongs to the field of biological small molecule active peptides, and relates to an antioxidant peptide derived from morel fruit body protein, specifically an antioxidant peptide derived from morel and its preparation method and application. Background technique [0002] Hypoxic-ischemic brain damage (Hypoxic-ischemic brain damage, HIBD) is one of the most common central nervous system diseases in newborns. It is mainly caused by the reduction or suspension of cerebral blood flow and oxygen after perinatal asphyxia. Injury or chronic long-term learning motor mental dysfunction has the characteristics of "three highs": high fatality rate, high morbidity rate, and high disability rate. Therefore, it is an urgent research topic to explore the pathogenesis of neonatal hypoxic ischemic encephalopathy and find safe and efficient prevention measures. Current studies confirm that apoptosis, as the final change in neurons among many influencing ...

Claims

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Application Information

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IPC IPC(8): C07K7/08C07K1/16C07K1/06C07K1/04A61K38/10A23L33/18A61P9/10A61P39/06
CPCA23V2002/00A61K38/00A23L33/18A61P9/10A61P39/06C07K7/08A23V2200/322A23V2200/30A23V2250/55
Inventor 熊川黄文丽金鑫李萍张璐
Owner SAAS BIOTECH & NUCLEAR TECH RES INST
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