Preparation method for manufacturing silkworm pupa protein of protein filament
A technology of silkworm chrysalis protein and silkworm chrysalis protein powder, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc. It can solve the problems of broken silkworm chrysalis protein filaments, recovery of silkworm chrysalis protein peptides, and low extraction rate of products. The effect of high quality, low production cost and simple production process
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Embodiment 1
[0025] A method for preparing silkworm pupa protein for producing protein filaments, the specific steps of the method are as follows:
[0026] (1) Preparation of alkaline solution of defatted silkworm pupa powder
[0027] Using commercially available defatted silkworm pupa as raw material, first send the defatted silkworm pupa into a pulverizer for pulverization, then collect the powder and pass through a 20-mesh sieve for separation, and collect the sieved powder (that is, the prepared defatted silkworm pupa powder); The crude product is returned to the crusher and then crushed. Then send the collected defatted silkworm pupa powder into the batching kettle, according to the mass fraction of defatted silkworm pupa powder (kg): the mass fraction of sodium hydroxide is 0.1% sodium hydroxide aqueous solution volume (L) ratio is 1:8 Proportion, the sodium hydroxide aqueous solution is pumped into the batching kettle, stirred and mixed evenly, and the alkaline solution of defatted silk...
Embodiment 2
[0037] A preparation method of silkworm pupa protein for producing protein filaments is the same as in Example 1, wherein:
[0038] In step (1), the powder is collected and separated through a 40-mesh sieve. The ratio of the mass of defatted silkworm pupa powder (kg): the volume of sodium hydroxide aqueous solution (L) with a mass fraction of sodium hydroxide of 1% is In the ratio of 1:12, the sodium hydroxide aqueous solution is pumped into the batching kettle.
[0039] In step (2), water vapor with a pressure of 0.25 MPa is introduced into the interlayer of the glass-lined reactor to heat the alkaline solution of defatted silkworm pupa powder in the glass-lined reactor to 75° C. and keep it for 0.75 h. Under stirring, continue to pass in water vapor to continue heating to 107°C, and keep the temperature for 1 hour before turning off the water vapor. Then, the mixed solution of the broken silkworm pupa globulin and chitin compound in the glass-lined reactor was cooled to 30°C und...
Embodiment 3
[0044] A preparation method of silkworm pupa protein for producing protein filaments is the same as in Example 1, wherein:
[0045] In step (1), the powder is collected and separated through a 60-mesh sieve. The ratio of the mass of defatted silkworm pupa powder (kg): the volume of sodium hydroxide aqueous solution (L) with a mass fraction of sodium hydroxide of 1% is The ratio of 1:16, the sodium hydroxide aqueous solution is pumped into the batching kettle.
[0046] In step (2), water vapor with a pressure of 0.4 MPa is introduced into the interlayer of the glass-lined reactor to heat the alkaline solution of defatted silkworm pupa powder in the glass-lined reactor to 80° C. and keep it for 1 hour. Under stirring, continue to pass in water vapor to continue heating to 115°C, and keep the temperature for 1.5 hours before turning off the water vapor. Then, the mixed solution of the broken silkworm pupa globulin and chitin compound in the glass-lined reactor was cooled to 20°C unde...
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