Method for detecting salmonellas in dehydrated garlic products

A technology for salmonella and products, applied in the field of microbial detection, can solve the problem of separation of pathogenic microorganisms, etc., and achieve the effects of rapid detection, strong specificity, and improved accuracy

Inactive Publication Date: 2012-10-03
安徽出入境检验检疫局检验检疫技术中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since food samples are often a solid-liquid multiphase mixture, it is difficult to separate a small amount of pathogenic microorganisms by conventional methods

Method used

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  • Method for detecting salmonellas in dehydrated garlic products
  • Method for detecting salmonellas in dehydrated garlic products

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Experimental program
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Embodiment Construction

[0024] Below in conjunction with embodiment, the present invention will be further described.

[0025] 1 Materials and instruments

[0026] 1.1 Materials

[0027] Salmonella species ( salmonella ) was provided by Beijing Lanbo Rui Company, Salmonella immune magnetic bead buffer was purchased from Shanghai Jiahe Biotechnology Co., Ltd., various media were provided by Beijing Luqiao Company, and samples were from Anhui Donggan Food Co., Ltd. for inspection samples; buffered peptone The configuration of water enrichment solution and selenite cystine enrichment solution refers to the national standard GB4789 series;

[0028] 1.2 Instruments

[0029] Desktop high-speed refrigerated centrifuge (BECKMAN 64R), vertical high-speed refrigerated centrifuge (BECKMAN J30I), micro-sampler: 0.5μL~10μL, 10μL~100μL, autoclave, VIDAS 30 automatic bacterial detection system

[0030] BAX? Q7 microbial detection system, constant temperature shaker.

[0031] 2 Methods and results

[0032]...

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Abstract

The invention discloses a method for detecting salmonellas in dehydrated garlic products by adopting the immunomagnetic separation technology. The method comprises the following steps: (1) adding garlic powder into sterilization diluent, obtaining filter residues through filtering by a film filter bag after homogenization; (2) oscillating and washing the residues by the sterilization diluent for three times, mixing washing liquid of the three times; (3) oscillating salmonella immunomagnetic bead buffer solution and the mixed washing liquid; (4) performing centrifugal separation to obtain centrifugation sediment; (5) adding buffer peptone water enrichment broth into the centrifugation sediment and culturing for 24 hours; and (6) taking the culture solution, adding selenite cystine enrichment broth for enrichment culture, and using the VIDAS system to detect the salmonellas in the dehydrated garlic products. According to the invention, the immunomagnetic separation technology is utilized to realize fast detection of the salmonellas in the dehydrated garlic products, and the technology has the advantages of high specificity and high sensitivity.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection, and in particular relates to a method for detecting Salmonella in dehydrated garlic products by adopting an immunomagnetic bead separation technology. Background technique [0002] Food hygiene and safety issues are closely related to everyone's health, and have always been concerned by governments and mainstream media around the world. In recent years, as people pay more and more attention to food safety, especially under the background of frequent food safety accidents in my country in recent years, and food quality and safety incidents related to Chinese export products are often reported in the newspapers, importing countries Food testing from China is getting stricter. my country is the world's largest producer and exporter of garlic (Allium sativum L.) and its products, and has become my country's first foreign currency export vegetable, with its exports increasing year by y...

Claims

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Application Information

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IPC IPC(8): C12Q1/10C12Q1/06
CPCY02A50/30
Inventor 余晓峰郑海松孙娟娟宗凯陈雪娇李云飞姚剑严轶
Owner 安徽出入境检验检疫局检验检疫技术中心
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