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Method for improving percent conversion of tylosin component A

A technology of tylosin and conversion rate, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of enhancing permeability, releasing feedback inhibition, and improving vitality

Active Publication Date: 2012-10-03
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are certain difficulties in the conversion process of components, and the relevant literature or patents on the difficulty of conversion of tylosin have not been reported so far

Method used

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  • Method for improving percent conversion of tylosin component A

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1) Selection of fermentation strains: Strepyomyce fradiae.

[0018] 2) 1m 3 The medium of the primary seed fermentation tank is: 10kg of bean cake powder, 0.5kg of betaine hydrochloride, 3kg of corn steep liquor, 0.2kg of diammonium hydrogen phosphate, 1.6kg of calcium carbonate, and 13L of soybean oil.

[0019] 3) 10m 3 The medium for the secondary seed fermentation tank is: 150kg of bean cake powder, 5kg of betaine hydrochloride, 2kg of diammonium hydrogen phosphate, 15kg of calcium carbonate, 80kg of mixed fish meal, 30kg of corn steep liquor, and 100L of soybean oil.

[0020] 4) 100m 3 Fermentation tank fermentation medium is: 2000L soybean oil, 1500kg soybean cake powder, 700kg mixed fish meal, 30kg diammonium hydrogen phosphate, 200kg calcium carbonate, 70kg betaine hydrochloride, 500g cobalt chloride, 50kg potassium chloride, 100kg sodium chloride, magnesium sulfate 80kg.

[0021] Concrete fermentation process steps are as follows:

[0022] 1) Fermentation c...

Embodiment 2

[0035] 1) Selection of fermentation strains: Strepyomyce fradiae.

[0036] 2) 1m 3 The medium of the primary seed fermentation tank is: 10kg of bean cake powder, 0.5kg of betaine hydrochloride, 3kg of corn steep liquor, 0.2kg of diammonium hydrogen phosphate, 1.6kg of calcium carbonate, and 13L of soybean oil.

[0037] 3) 10m 3 The medium for the secondary seed fermentation tank is: 150kg of bean cake powder, 5kg of betaine hydrochloride, 2kg of diammonium hydrogen phosphate, 15kg of calcium carbonate, 80kg of mixed fish meal, 30kg of corn steep liquor, and 100L of soybean oil.

[0038] 4) 100m 3 The fermentation medium of the fermentation tank is: soybean oil 2300L, soybean cake powder 1600kg, mixed fish meal 750kg, diammonium hydrogen phosphate 35kg, calcium carbonate 230kg, betaine hydrochloride 80kg, cobalt chloride 550g, potassium chloride 55kg, sodium chloride 120kg, magnesium sulfate 85kg.

[0039] Concrete fermentation process steps are as follows:

[0040] 1) Fer...

Embodiment 3

[0053] 1) Selection of fermentation strains: Strepyomyce fradiae.

[0054] 2) 1m 3 The medium of the primary seed fermentation tank is: 10kg of bean cake powder, 0.5kg of betaine hydrochloride, 3kg of corn steep liquor, 0.2kg of diammonium hydrogen phosphate, 1.6kg of calcium carbonate, and 13L of soybean oil.

[0055] 3) 10m 3 The medium of the secondary seed fermentation tank is: 150kg of bean cake powder, 50kg of betaine hydrochloride, 20kg of diammonium hydrogen phosphate, 15kg of calcium carbonate, 80kg of mixed fish meal, 30kg of corn steep liquor, and 100L of soybean oil.

[0056] 4) 100m 3 Fermentation tank fermentation medium is: 2500L soybean oil, 1700kg bean cake powder, 800kg mixed fish meal, 30kg diammonium hydrogen phosphate, 250kg calcium carbonate, 80kg betaine hydrochloride, 600g cobalt chloride, 70kg potassium chloride, 130kg sodium chloride, magnesium sulfate 90kg.

[0057] Concrete fermentation process steps are as follows:

[0058] 1) Fermentation cul...

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Abstract

The invention relates to a method for improving the percent conversion of a tylosin component A. With the method, streptomyces fradiae is used as a producing strain and is fermented to produce the tylosin by first-level seed tank fermentation culture, second-level seed tank fermentation culture and fermentation tank fermentation culture. The method is characterized in that nonionic surfactant of which the mass concentration is 0.5-2.5% is added into a fermentation culture medium after the fermentation tank fermentation culture enters a stable period after a logarithmic growth phase is finished. The nonionic surfactant is added into the culture medium during a fermentation culture period to the stable period so as to enhance the permeability of a mycelium cell, so that a metabolin in a cell is accelerated to quickly release out of the cell to relieve the feedback inhibition of macrosin methyltransferase and improve the enzyme activity, and finally, a tylosin component C is accelerated to quickly covert into the tylosin component A. The tylosin component A in the current industrial production generally accounts for 80-85%, and the content of the tylosin component A can finally achieve 90-94% according to the method disclosed by the invention.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation engineering, in particular to a method for improving the conversion rate of tylosin A. Background technique [0002] Tylosin is a macrolide antibiotic obtained by fermentation and extraction of Streptomyces freundii. It has three forms: tylosin base, phosphate and tartrate. Tylosin is a high-efficiency, non-residue antibacterial and growth-promoting agent for poultry and livestock. It has a unique curative effect on chicken septicemia, porcine epidemic pneumonia and other diseases, and has obvious promoting effect on the growth of poultry and livestock. As an animal-specific antibiotic, tylosin avoids the problem of cross-resistance caused by shared antibiotics between humans and animals, and is used for the prevention and treatment of swine and poultry mycoplasma diseases. [0003] The key factor affecting the biotransformation of tylosin A in the fermentation production process...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/62C12R1/54
Inventor 王义任勇刘建成王文超徐淑芬
Owner 宁夏泰瑞制药股份有限公司
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