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Corn WUS1 gene promoter and application thereof

A gene and polynucleotide technology, applied in the field of promoting the expression of target genes in plant tissues, corn WUS1 gene promoter, can solve the problems of biological insecurity and achieve the effect of strong biological safety

Inactive Publication Date: 2014-01-15
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the gene promoter sequences cloned from viruses may be potentially biologically unsafe when applied to plant genetic engineering. Therefore, it is imperative to clone highly active promoters from plants themselves.

Method used

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  • Corn WUS1 gene promoter and application thereof
  • Corn WUS1 gene promoter and application thereof
  • Corn WUS1 gene promoter and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Materials

[0026] The methods used in this example are routine methods known to those skilled in the art unless otherwise specified; the primers used are all synthesized by Shanghai Jierui Bioengineering Co., Ltd., and the sequencing is carried out by Invitrogen Company; PCR kit, ligation kit and The endonucleases in the vector construction process were purchased from Zibao Bioengineering Co., Ltd., the plasmid extraction kit and gel recovery kit were purchased from Quanshijin Biotechnology Co., Ltd., and the specific operation methods were carried out according to the kit instructions; Trans5α chemically competent cells and EH105 Agrobacterium competent cells were purchased from Quanshijin Biotechnology Co., Ltd.; pMD18-T Vector and pCAMBIA1301 were purchased from Bao Bioengineering Co., Ltd., and the vector maps were as follows: figure 1 and figure 2 Shown; YEP liquid medium formula is: beef extract 10 g, yeast extract 10 g, NaCl 5 g, pH 7.0, the corresponding ...

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Abstract

The invention provides a corn WUS1 gene promoter, which comprises a nucleotide sequence composed of a sequence shown in SEQ ID NO.3 or a nucleotide sequence complementary with the sequence shown in the SEQ ID NO.3. The invention also provides a carrier and a host cell, which contain the promoter. The invention also discloses use of the promoter. The gene promoter has the advantages that the WUS1 gene promoter is obtained by cloning, the condition that the promoter can promote a target gene to be expressed in root, stipule, leaf and stem of rice is proved, and condition that the efficiency of expression of the target gene driven by the promoter is equivalent to that of (CaMV) 35S promoter is indicated by comparing with positive contrast of a transformed pCAMBIA1301 carrier. The corn WUS1 gene promoter is a new promoter applied to plant genetic engineering and derived from plant, and has strong biosecurity. The cloning of the promoter has important reference value as to research of a corn growth mechanism at the same time.

Description

technical field [0001] The present invention relates to the field of botany and genetic engineering technology, in particular, relates to corn WUS1 A gene promoter and its application, especially the application in promoting the expression of a target gene in a plant tissue. Background technique [0002] The purpose of plant genetic engineering is to introduce foreign genes into recipient plants to make them express correctly and effectively, and a suitable promoter is the key factor for the effective expression of foreign genes. At present, promoters from many different sources have been used in genetic engineering fields such as plant quality improvement genetic engineering, disease resistance breeding genetic engineering, and plant bioreactors. A large group of these promoters is isolated from Agrobacterium tumefaciens, and another large group is the promoters derived from the plant itself. However, the expression level of the target gene controlled by the above-mention...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/84C12N5/10A01H5/00
Inventor 程备久竹荣梓朱苏文项艳曹建刚姜翠萍段思宇
Owner ANHUI AGRICULTURAL UNIVERSITY
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