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Composite material nanosphere as well as preparation method and application thereof

A composite material and nanosphere technology, applied in the field of biomedicine, can solve problems such as limited curative effect, and achieve the effects of gene transfection rate, antiserum, good biocompatibility, low toxicity, and high photothermal conversion efficiency.

Pending Publication Date: 2022-05-10
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the efficacy of a single treatment in cancer is still limited, and the combined treatment of multiple treatments is more effective

Method used

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  • Composite material nanosphere as well as preparation method and application thereof
  • Composite material nanosphere as well as preparation method and application thereof
  • Composite material nanosphere as well as preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0096] The preparation method of the above-mentioned ultrasonically visualized multifunctional nanocarrier with photothermal treatment and enhanced macrophage gene transfection comprises the following steps:

[0097] 1) Preparation of polylactic acid-glycolic acid entrapped perfluoro-n-pentane nanoparticles:

[0098] Proportionally, add 60-100mg polylactic acid-glycolic acid white powder into 2-6mL dichloromethane, and ultrasonically disperse in an ultrasonic cleaner with a power of 30-50Hz until fully dissolved. Add 100-300 μL of perfluoro-n-pentane to the above solution, and use an ultrasonic breaker with a power of 100-200W to sonicate for 2-3 minutes under an ice bath; add the above mixed solution drop by drop to 20-30ml pre-cooled In the aqueous solution of 2wt% polyvinyl alcohol, under an ice bath, use a power ultrasonic breaker with a power of 300-500W to ultrasonically break again for 2-3 minutes, stir magnetically at room temperature for 4-5 hours, and take the mixed ...

Embodiment 1

[0113] 1) Preparation of polylactic acid-glycolic acid entrapped perfluoro-n-pentane nanoparticles:

[0114] Proportionally, 60 mg of polylactic acid-glycolic acid white powder was added to 2 mL of dichloromethane, and ultrasonically dispersed in a 30 Hz power ultrasonic cleaner until fully dissolved. Add 100 μL of perfluoro-n-pentane to the above solution, and use an ultrasonic breaker with a power of 100 W to sonicate for 2 minutes under an ice bath; add the above mixed solution drop by drop to 20 ml of pre-cooled 2 wt% polyvinyl alcohol aqueous solution , in an ice bath with an ultrasonic breaker with a power of 300W, ultrasonicated again for 2 minutes, stirred magnetically at room temperature for 4 hours, and the mixed solution was centrifuged with a low-temperature high-speed centrifuge (10000rpm, 15min, 4°C), washed, and precipitated in 5ml pure Dispersion in water is polylactic acid glycolic acid entrapped perfluoro-n-pentane nanoparticle aqueous solution.

[0115] 2) ...

Embodiment 2

[0121] 1) Preparation of polylactic acid-glycolic acid entrapped perfluoro-n-pentane nanoparticles:

[0122] Proportionally, 100 mg of polylactic acid-glycolic acid white powder was added to 4 mL of dichloromethane, and ultrasonically dispersed in an ultrasonic cleaner with a power of 50 Hz until fully dissolved. Add 200 μL of perfluoro-n-pentane to the above solution, and use an ultrasonic breaker with a power of 160 W to sonicate for 3 minutes under an ice bath; add the above mixed solution drop by drop to 25 ml of pre-cooled 2 wt% polyvinyl alcohol aqueous solution , in an ice bath with an ultrasonic breaker with a power of 400W, ultrasonicated again for 2 minutes, stirred magnetically at room temperature for 4 hours, and the mixed solution was centrifuged with a low-temperature high-speed centrifuge (13000rpm, 20min, 4°C), washed, and precipitated in 5ml pure Dispersion in water is polylactic acid glycolic acid entrapped perfluoro-n-pentane nanoparticle aqueous solution. ...

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Abstract

The invention discloses a composite nanosphere as well as a preparation method and application thereof. The composite material nanosphere comprises an inner core particle, a metal nano shell layer, a mercapto acid modification layer and a polyethyleneimine compound modification layer, the inner core particles are formed by coating a fluorocarbon compound with a film-forming substance; the surface of the inner core particle is coated with the metal nano shell layer; the sulfydryl acid modification layer is connected to the metal shell layer through sulfydryl; the polyethyleneimine compound layer is connected to the mercapto acid modified layer through an amido bond. The composite material nanosphere has multiple functions of photothermal therapy, enhancement of macrophage gene transfection, ultrasonic enhancement of imaging and the like.

Description

technical field [0001] The application relates to a composite material nanosphere and its preparation method and application, belonging to the technical field of biomedicine. Background technique [0002] In recent years, immunotherapy has significantly improved the prognosis of cancer patients, especially the gospel of advanced patients. Inhibiting the polarization of tumor-associated macrophages (TAMs) is one of the important approaches for tumor immunotherapy. TAMs are innate immune effector cell populations infiltrating into tumor tissue, accounting for 5-72% of the whole tumor tissue cells. TAMs can be induced into different phenotypes by different factors, one of which is M1-type TAMs with anti-tumor effect, and the other is M2-type TAMs that promote tumor proliferation and metastasis. M2-type TAMs play an important role in tumor immunity, which can regulate inflammatory responses, adaptive immunity, clear debris, promote angiogenesis, tissue remodeling repair, stimu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/58A61K47/69A61K41/00A61K49/22A61P35/00B82Y5/00B82Y30/00B82Y40/00A61K31/713
CPCA61K47/58A61K47/6925A61K49/223A61K41/0052A61K49/225A61K31/713A61P35/00B82Y5/00B82Y30/00B82Y40/00A61K2300/00
Inventor 聂芳吕文豪吴昊朱阳阳李琪汪延芳
Owner LANZHOU UNIVERSITY
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