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Method for constructing tree shrew nonalcoholic simple fatty liver animal models

A simple fatty liver, animal model technology, applied in animal husbandry and other directions, can solve the problem of not being able to comprehensively and accurately reflect human fatty liver, and achieve good application prospects, high modeling efficiency, and short modeling time.

Inactive Publication Date: 2014-01-01
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the establishment of different types of non-alcoholic fatty liver animal models, hepatitis and liver cirrhosis are more common, while simple fatty liver is rarely reported
On the other hand, due to the complexity of human liver function and the racial differences of experimental animals, the selection of animal age, the control of feed, the time of modeling, and the lack of uniform standards for model judgment indicators, any experimental animal model None of them can fully and accurately reflect the essence of human fatty liver, especially the essence of simple fatty liver

Method used

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  • Method for constructing tree shrew nonalcoholic simple fatty liver animal models
  • Method for constructing tree shrew nonalcoholic simple fatty liver animal models
  • Method for constructing tree shrew nonalcoholic simple fatty liver animal models

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Randomly select 20 adult males and 20 female tree shrews as model animals. The adult males weigh 120-140 grams, and the females weigh 110-130 grams. The tree shrews eat freely with high-fat feed. Add 20g of cholesterol and 200g of lard to one kilogram of common feed. During the modeling period, all animals had free access to water.

[0026] The modeling period was 14 weeks in total. After 7 weeks, 60% of the tree shrews showed obvious simple fatty liver symptoms, and after 14 weeks, 80% of the tree shrews showed obvious simple fatty liver symptoms, and 20% of them showed severe simple fatty liver symptoms. Tree shrews have about 95% fatty liver cells.

[0027] During the whole experiment, no animal died.

Embodiment 2

[0029] Randomly select 30 adult male tree shrews and 30 female tree shrews each as experimental animals, with adult males weighing 120-140 grams and adult females weighing 110-130 grams. Among them, 10 adult males and 10 female tree shrews were used as control animals and fed freely. Ordinary feed, the nutrient composition of ordinary feed is: 19%<crude protein<21%, 4%<crude fat<5%. The remaining 20 male and 20 female animals were used as model animals, and the tree shrews were fed freely with high-fat feed, the composition of which was: 20 g of cholesterol and 200 g of lard per kilogram of common feed. During the modeling period, all animals had free access to water.

[0030] During the whole experiment, no animal died.

[0031] (2) Evaluation method and results of tree shrew fatty liver diagnosis model

[0032] The method includes serum biochemical markers, imaging, and histopathological diagnosis.

[0033] 1. Serum biochemical markers mainly include serum alanine aminotr...

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Abstract

Disclosed is a method for constructing tree shrew nonalcoholic simple fatty liver animal models. Tree shrew serve as a model animal, a high-fat diet consists of 1000g of normal diets, 20g of cholesterol and 200g of lard and is used for freely feeding the tree shrews, and the tree shrews can drink purified water freely. After seven weeks, 60% of the tree shrews have an obvious fatty liver symptom; after 14 weeks, 80% of the tree shrews have the obvious fatty liver symptom, wherein 20% of the tree shrews have a severe fatty liver symptom, and liver cells of about 95% of the tree shrews have pimelosis; and an analysis result shows that the method is short in modeling time, high in success ratio, safe, reliable and capable of being used for drug research and efficacy assessment of nonalcoholic fatty livers and particularly for the drug research and the efficacy assessment of simple fatty livers.

Description

technical field [0001] The invention belongs to a method for establishing an animal model, in particular to a method for using animals to construct an animal model of non-alcoholic fatty liver. Background technique [0002] The incidence of fatty liver has risen rapidly in Europe, America and China in recent years, becoming the second largest liver disease after viral hepatitis. The average incidence rate of fatty liver in white-collar workers, government officials, senior intellectuals and other professional groups is about 25%; the incidence rate of fatty liver in obese people and patients with type 2 diabetes is about 50%; in alcoholics and alcoholics The incidence of fatty liver is about 58%; the incidence of fatty liver is about 60% among sub-healthy people who often suffer from insomnia, fatigue, lack of food, and gastrointestinal dysfunction. [0003] Non-alcoholic fatty liver disease refers to a clinicopathological syndrome characterized by diffuse bullous steatosis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/00
Inventor 牛昱宇王宏司维郭祥玉司晨洋王芳张琼书施桂英
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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