Fermentation culture medium of dextranase-producing bacteria and culture method thereof

A technology of dextranase and fermentation medium, which is applied in the field of bioengineering and can solve the problems of low fermentation level and low fermentation enzyme activity unit.

Active Publication Date: 2013-07-10
XINYU PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fermentation level and fermentation enzyme activity unit of the existing dextranase-producing bacteria fermentation medium are relatively low, and the enzyme activity unit is less than 500u / ml

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Fermentation medium formula 1 with peanut cake powder added: 0.5% peptone, K 2 HPO 4 0.5%, KCl 0.02%, FeSO 4 0.002%, peanut cake powder 0.1%, dextran 3.0%, and the balance is well water.

[0018] Fermentation medium control group 1 without peanut cake powder: peptone 0.5%, K 2 HPO 4 0.5%, KCl 0.02%, FeSO 4 0.002%, dextran 3.0%, and the balance is well water.

[0019] Two groups of small experiments were carried out in a 50L fermenter. Sand spores were inoculated in the slant medium at a temperature of 28°C and a humidity of 40-60% for 5-6 days; mature slant spores were inserted into the shake flask seed medium at a temperature of Cultivate for 40-60 hours at 28°C, humidity 40-60%, rotation speed 220-240RPM; inoculate shake flask seeds at 1% inoculation amount in seed tanks, and cultivate at 28°C for 40-60 hours; inoculate with 1% inoculum amount In the fermentation medium, culture at 28°C for 120-150 hours to end the fermentation.

[0020] The enzyme activity...

Embodiment 2

[0022] Fermentation medium formula 2 with peanut cake powder added: 0.3% peptone, K 2 HPO 4 0.4%, KCl 0.015%, FeSO 4 0.0015%, peanut cake powder 0.2%, dextran 2.8%, and the balance is well water.

[0023] Fermentation medium control group 2 without peanut cake powder: peptone 0.3%, K 2 HPO 4 0.4%, KCl 0.015%, FeSO 4 0.0015%, dextran 2.8%, and the balance is well water.

[0024] Two groups of small experiments were carried out in a 50L fermenter. Sand spores were inoculated in the slant medium at a temperature of 28°C and a humidity of 40-60% for 5-6 days; mature slant spores were inserted into the shake flask seed medium at a temperature of Cultivate for 40-60 hours at 28°C, humidity 40-60%, rotation speed 220-240RPM; inoculate shake flask seeds at 1% inoculation amount in seed tanks, and cultivate at 28°C for 40-60 hours; inoculate with 1% inoculum amount In the fermentation medium, culture at 28°C for 120-150 hours to end the fermentation.

[0025] The enzyme acti...

Embodiment 3

[0027] Fermentation medium formula 3 with peanut cake powder added: 0.2% peptone, K 2 HPO 4 0.3%, KCl 0.01%, FeSO 4 0.001%, 0.5% peanut cake powder, 2.5% dextran, and the balance is well water.

[0028] Fermentation medium control group 3 without peanut cake powder: peptone 0.2%, K 2 HPO 4 0.3%, KCl 0.01%, FeSO 4 0.001%, dextran 2.5%, and the balance is well water.

[0029] Two groups of small experiments were carried out in a 50L fermenter. Sand spores were inoculated in the slant medium at a temperature of 28°C and a humidity of 40-60% for 5-6 days; mature slant spores were inserted into the shake flask seed medium at a temperature of Cultivate for 40-60 hours at 28°C, humidity 40-60%, rotation speed 220-240RPM; inoculate shake flask seeds at 1% inoculation amount in seed tanks, and cultivate at 28°C for 40-60 hours; inoculate with 1% inoculum amount In the fermentation medium, culture at 28°C for 120-150 hours to end the fermentation.

[0030] The enzyme activity...

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Abstract

The invention discloses a fermentation culture medium of dextranase-producing bacteria and a culture method thereof. The fermentation culture medium comprises the following components in percentage by weight: 0.2-0.5% of peptone, 0.3-0.5% of K2HPO4, 0.01-0.02% of KCl, 0.001-0.002% of FeSO4, 0.1-0.5% of peanut meal, 2.5-3.0% of dextran and the balance of well water. The culture method disclosed bythe invention comprises the following steps of: adding an appropriate amount of the peanut meal into the fermentation culture medium of the dextranase-producing bacteria, taking the peanut meal as a slow impact nitrogen source growth thallus, and taking the dextran as an enzyme production inducer, so that the fermentation level of the dextranase can be significantly improved, enzyme activity units can be increased by 45% to the greatest extent, and the culture method has the advantages of strong operability, low input and high output.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a fermentation medium of a dextranase-producing bacterium with increased enzyme activity units and a culture method thereof. Background technique [0002] Dextranase is a new enzyme that can catalyze the decomposition of dextran into glucose or oligosaccharides. It is a protein. Dextranase has important uses in the pharmaceutical industry and food industry. The study of dextranase is of great significance for the prevention and treatment of dental caries and the development of dental caries vaccine; The filterability of sugar products can increase the recovery rate of sugar and reduce the viscosity, so the market prospect of this product is broad. The fermentation level and fermentation enzyme activity unit of the existing dextranase-producing bacteria fermentation medium are relatively low, and the enzyme activity unit is less than 500u / ml. Contents of the invention ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/46C12N1/14C12R1/80
Inventor 曹新年杨春丽蔡惠丽李为全刘瑞华
Owner XINYU PHARM CO LTD
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