Method for improving fermentation yield of rifamycin SV

Abstract

The invention discloses a method for improving the fermentation yield of rifamycin SV. In the process of fermentation culture, oxalate with the final concentration of 0.2-0.5g / L and soybean oil with the final concentration of 2-5mL / L are sterilely added into a fermentation tank the fermentation culture cycle of amycolatopsis mediterranei of which is 60-80 hours. The titer of the rifamycin SV is increased by 3.0% on average, the titer is increased by 5.4% on average in case of one billion tanks, carbohydrate supplementation is decreased by 17% and the raw material cost of every tank is saved by***. The supplementary materials in the method are all agricultural and industrial commodities with high affordability. In addition, the operational process is simple and the controllability is strong, thus the method is applicable to large-scale fermentation production, the production increase effect is stable and the method conforms to the green development requirement of energy conservation in fermentation industry by the state.

Description

technical field [0001] The invention relates to a preparation method of rifamycin, in particular to a method for increasing the fermentation yield of rifamycin SV. Background technique [0002] As a bulk antibiotic raw material for tuberculosis treatment, the fermentation unit of domestic rifamycin SV has been hovering around 5000 U / mL. The physiological state of the bacteria at the industrial production level is closely related to the process control. There is an interaction effect among the glycolysis, pentose phosphate pathway, tricarboxylic acid cycle pathway and rifamycin precursor synthesis pathway. It is determined that the latter is related to the specific The corresponding relationship of metabolic bottleneck will guide the optimization index and specificity of rifamycin SV fermentation production process, which has important practical significance for improving the control and execution of production efficiency. [0003] The carbon and nitrogen metabolic pathways ...

AI Technical Summary

Problems solved by technology

[0004] At present, there are many studies on improving the biosynthesis efficiency of rifamycin at home and abroad, including: strain improvement, process optimization, and replacement of cheap raw materials. Soybean oil and soybean oil are supplemented into the fermentation culture of Amycolatopsis mediterranei at the optimal time to regulate the synthetic metabolic flow of rifamycin SV precursor and improve its synthetic efficiency. There are no relevant literature and published patent reports.