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Method for conveying proteins or antigens to mammal intestinal tract in targeted way by using transgenic coccidian

A mammalian and transgenic technology, applied in protozoa, peptide/protein components, introduction of foreign genetic material using vectors, etc., can solve the problems of complex operation and low biosafety, and achieve simple operation, easy passage and purification, high The effect of biosecurity

Inactive Publication Date: 2012-09-12
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to overcome the problems of complicated operation and low biological safety existing in the existing mucosal transport antigen or protein system, and to provide a new method of using transgenic coccidia to target the delivery of protein or antigen to the intestinal tract of mammals

Method used

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  • Method for conveying proteins or antigens to mammal intestinal tract in targeted way by using transgenic coccidian
  • Method for conveying proteins or antigens to mammal intestinal tract in targeted way by using transgenic coccidian
  • Method for conveying proteins or antigens to mammal intestinal tract in targeted way by using transgenic coccidian

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Embodiment

[0028] In this example, the transgenic Eimeria tenella (Eimeria tenella) was used as a carrier to express a classic mucosal adjuvant CTB, and it was targeted to be transported in the intestinal cavity of mice, so as to enhance the mouse's response to chicken balls. effects of the worm immune response.

[0029] 1. Carrier construction

[0030] Log in to the codon usage database website http: / / www.kazusa.org.jp / codon / to query the usage frequency of each codon during protein translation of Eimeria tenella; on the OPTIMIZER server (http: / / genomes.urv .es / OPTIMIZER / ) After completing operations such as entering the CTB gene sequence of Vibrio cholerae and the codon usage frequency table of Eimeria tenella, the modified sequence of CTB can be obtained, and the CTB gene selection completely uses the most suitable Codon optimization scheme. The optimized sequence was submitted to a DNA sequencing synthesis company for direct synthesis of the full-length sequence (Seq ID No.2). Dig...

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Abstract

The invention provides a method for conveying proteins or antigens to a mammal intestinal tract in a targeted way by using a transgenic coccidian. The coccidian is taken as a carrier for conveying proteins or antigens in a targeted way, and foreign proteins or antigens are expressed in the sporangium cavity of the coccidian by performing transgenic operation on the coccidian. The invention provides a novel application of the transgenic coccidian. The foreign proteins are targeted onto the sporangium cavity of the coccidian by using an excretion signal peptide of the coccidian, an oocyst breaks and releases sporangium under the mechanical creeping action of stomach in the stomach, the sporangium breaks and releases daughter spores under the digestion actions of gall and pancreatic enzyme in a small intestine, proteins in the sporangium are released into an intestinal tract, and the denaturation degradation effect of gastric juice on the proteins is avoided, so that proteins in the sporangium are directly contacted with intestinal mucosa, and the aim of activating enteric immune response or treating enteric diseases is fulfilled. The coccidian used in the method has the advantages of high type specificity, high biological safety, capability of releasing foreign proteins into intestinal tracts in a targeted way, suitability for oral application, easiness for operating and wide application prospect.

Description

technical field [0001] The invention relates to the application of the transgenic coccidia, in particular to a method for using the transgenic coccidia to deliver proteins or antigens to the intestinal tract of mammals. Background technique [0002] The mucous membrane is the main site for most pathogens to invade the body, so targeting foreign antigens to the mucous membrane to stimulate a specific mucosal immune response is an important strategy to resist pathogenic invasion. There are two main types of common mucosal transport antigen or protein systems: one is a synthetic system, which encapsulates purified antigens in microspheres, liposomes, nanoparticles or immunostimulatory complexes; the other is a living Vector system, including some attenuated bacterial strains or virus strains, etc. However, both systems have disadvantages—the former needs to manually prepare and package the purified antigen, which is complicated to operate and requires a relatively large amount...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K38/00A61K47/46C12N15/63C12N1/11
Inventor 索勋刘贤勇殷光文秦梅
Owner CHINA AGRI UNIV
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