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In-vitro rapid propagation method for tissue culture of broad-leafed notopterygium

A technology of notopterygia broadleaf and tissue culture, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of tissue culture that have not been reported yet, and achieve good biological characteristics, good synchronization, and fast emergence Effect

Inactive Publication Date: 2012-09-12
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The literature has reported the tissue culture of many alpine wild medicinal materials, such as Saussurea radix L., Rhodiola rosea, etc., but the tissue culture of the root buds of wild Qianghuo broad-leaved plant as explants has not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 A method for in vitro rapid propagation of Notopterygia broadleaf tissue culture, comprising the following steps:

[0028] (1) Selection and disinfection of explants: use the root buds of Notopterygium broadleaf as explants, wash them with running water for 15 minutes, soak them in alcohol with a volume concentration of 75% for 20 seconds, and then rinse them with sterile deionized water 2 to 3 times, then put the washed explants in mercuric chloride with a mass concentration of 0.1%, soak and sterilize for 8 minutes, rinse with sterile deionized water for 3 times, and finally dry the water with sterile filter paper. Single buds of explants after disinfection were obtained.

[0029] (2) Inoculation of explants: Inoculate single buds of sterilized explants on the induction bud differentiation medium, wherein one sterilized single bud of explants is inoculated in every 25ml of induction bud differentiation medium; at a temperature of 20°C ±2℃, the light s...

Embodiment 2

[0038] Example 2 A method for in vitro rapid propagation of Notopterygia broadleaf tissue culture, comprising the following steps:

[0039] (1) Selection and disinfection of explants: use the root buds of Notopterygium broadleaf as explants, wash them with running water for 20 minutes, soak them in alcohol with a volume concentration of 75% for 25 seconds, and then rinse them with sterile deionized water 2 to 3 times, then put the washed explants in mercuric chloride with a mass concentration of 0.15%, soak and sterilize for 10 minutes, and rinse with sterile deionized water for 3 times, and finally dry the water with sterile filter paper. Single buds of explants after disinfection were obtained.

[0040] (2) Inoculation of explants: Inoculate single buds of sterilized explants on the induction bud differentiation medium, wherein one sterilized single bud of explants is inoculated in every 25ml of induction bud differentiation medium; at a temperature of 20°C ±2℃, the l...

Embodiment 3

[0049] Example 3 A method for in vitro rapid propagation of Notopterygia broadleaf tissue culture, comprising the following steps:

[0050](1) Selection and disinfection of explants: use the root buds of Notopterygium broadleaf as explants, wash them with running water for 25 minutes, soak them in alcohol with a volume concentration of 75% for 30 seconds, and then rinse them with sterile deionized water 2 to 3 times, then put the washed explants in mercuric chloride with a mass concentration of 0.2%, soak and sterilize for 12 minutes, and rinse 4 times with sterile deionized water, and finally dry the water with sterile filter paper. Single buds of explants after disinfection were obtained.

[0051] (2) Inoculation of explants: Inoculate single buds of sterilized explants on the induction bud differentiation medium, wherein one sterilized single bud of explants is inoculated in every 25ml of induction bud differentiation medium; at a temperature of 20°C ±2℃, the light s...

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Abstract

The invention relates to an in-vitro rapid propagation method for tissue culture of broad-leafed notopterygium. The method comprises the following steps: (1) selection and sterilization on explants: based on radical buds of broad-leafed notopterygium as explants, carrying out soaking sterilization and washing on the radical buds so as to obtain sterilized single buds of explants; (2) explant inoculation: inoculating the sterilized single buds of explants to an bud differentiation inducing medium so as to form 2-3 fasciculate buds; (3) subculture multiplication: inoculating the 2-3 fasciculate buds to a subculture multiplication medium so as to form fasciculate seedlings of broad-leafed notopterygium; (4) rooting induction: after carrying out plant division on the fasciculate seedlings of broad-leafed notopterygium, transplanting the fasciculate seedlings of broad-leafed notopterygium to a rooting medium to culture so as to form complete regenerated plantlets; (5) carrying out sterilization on nursing mediums; and (6) plant transplanting: directly transplanting the complete regenerated plantlets to the sterilized nursing mediums, so that after exercising seedlings, the plantlets grow into normal broad-leafed notopterygium plants. The method disclosed by the invention solves the problem that the broad-leafed notopterygium is overlong in seedling growing period and low in propagation coefficient; the method is easy to operate; and the method can be applied to rapid seedling growing in factories.

Description

technical field [0001] The invention relates to tissue culture and in vitro rapid propagation of a plant, in particular to a method for in vitro rapid propagation of notopterygia broadleaves tissue culture. Background technique [0002] Notopterygium belongs to the genus Notopterygium (Umbelliferae) of the family Umbelliferae ( Notopterygium ) perennial plant is an important and endangered plant unique to my country. It is one of the most commonly used medicinal materials in Chinese, Tibetan and Qiang medicines, and it is also a Chinese and Tibetan medicinal material with a large market gap. Notopterygium broad-leaf is used as a medicine with rhizomes and roots. It is pungent in taste and warm in nature. Notopterygium broad-leaf has been recorded in Chinese pharmacopoeias in previous editions. According to the 2005 edition of the Pharmacopoeia of the People's Republic of China, it is a perennial herb of Umbelliferae (Umbelliferae). Notopterygium incisum Ting ex H.T. Chang...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 周国英徐文华刘卫根贺丽华赵晓辉
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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