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Application of curcumin to preparation of drugs for treating cerebral ischemia/reperfusion injuries

A cerebral ischemia-reperfusion, curcumin technology, used in drug combination, cardiovascular system diseases, digestive system and other directions, can solve problems such as brain tissue reperfusion injury, etc., to improve liver and kidney function damage, neurological function. State-improving, low-cost effects

Inactive Publication Date: 2012-09-05
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Improving and restoring cerebral blood supply and neuroprotection are two important strategies for the treatment of ischemic stroke. The currently recognized effective method is thrombolytic therapy, but limited by the time window of thrombolytic therapy and treatment conditions, only about 3% of patients This treatment can be performed, and although thrombolytic therapy can establish blood flow reperfusion in the ischemic area, it cannot solve the brain tissue reperfusion injury caused by ischemia

Method used

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  • Application of curcumin to preparation of drugs for treating cerebral ischemia/reperfusion injuries
  • Application of curcumin to preparation of drugs for treating cerebral ischemia/reperfusion injuries
  • Application of curcumin to preparation of drugs for treating cerebral ischemia/reperfusion injuries

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 curcumin injection and mouse focal cerebral ischemia reperfusion (MCAO) model preparation

[0027] (1) Preparation of Curcumin Injection

[0028] ① Povidone (PVP) curcumin solid dispersion:

[0029] Mass ratio (curcumin:PVP=1:8) PVP 24g, dissolved in 40ml absolute ethanol, magnetically stirred

[0030] Dissolve 3g of curcumin in 3ml of absolute ethanol, add it dropwise to the PVP solution, wash the bottle with 10ml of absolute ethanol, stir manually, pour it into a 250ml beaker with a large rotor and stir well, freeze-dry for 4 hours after stirring.

[0031] ② Curcumin solid dispersion solution:

[0032] Weigh 900mg of curcumin solid dispersion, add 5ml of normal saline for injection, that is curcumin 20mg / ml, in a brown bottle, it is transparent orange red, viscous, take 20mg / ml 1ml+ normal saline 7ml is 2.5mg / ml ml of application solution.

[0033] (2) Making mice focal cerebral ischemia-reperfusion injury model: 60 male Kunming mice weighing about 25...

Embodiment 2

[0042] Example 2 Histopathological changes of cerebral ischemia-reperfusion groups in mice

[0043] (1) After 2 hours of HE staining ischemia and 24 hours of reperfusion, the mice were anesthetized with 1% sodium pentobarbital (6mg / 100g), opened the chest and cut the right atrial appendage, and perfused sequentially through the left ventricle (containing 0.4% heparin) with 0.9% physiological Saline 30ml, 4% paraformaldehyde 30ml, the brain was removed immediately, post-fixed in 10% paraformaldehyde solution for 48h. Paraffin embedding was carried out by conventional methods, and 2 mm thick coronal sections were taken at the forehead thickness of 5 mm for HE section staining. The pathological changes of the cerebral cortex on the ischemic side were observed by light microscope.

[0044] (2) HE staining results, such as figure 2 As shown in the light microscope, the number and shape distribution of nerve cells in the sham operation group were normal; the arrangement of cells ...

Embodiment 3

[0045] Example 3 Immunohistochemistry of NSE, Cox-II, and Bcl-2 in brain tissue of mice after cerebral ischemia-reperfusion

[0046] (1) Immunohistochemical detection of NSE, Cox-II, and Bcl-2 expressions According to the results of HE staining, the ischemic infarct was determined to be sectioned, and the paraffin tissue sections were dewaxed, stained according to the immunohistochemical SABC method, and 3% H 2 o 2 Block endogenous peroxidase activity for 30 min, wash with PBS for 5 min x 2 times, and distilled water for 5 min x 2 times. Citrate buffer (pH 6.0) was heat-repaired at 92°C for 5 minutes, allowed to stand for 5 minutes, heat-repaired again for 5 minutes, washed with PBS for 5 minutes×2 times, and washed with distilled water for 5 minutes×2 times. 1.5% goat serum was sealed and incubated in a wet box for 1h (37°C), discarded without washing, and primary antibodies (rabbit anti-mouse Cox-II (1:50) and Bcl-2 (1:200) polyclonal antibodies) were added dropwise 37 Incub...

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Abstract

The invention discloses application of curcumin to preparation of drugs for treating cerebral ischemia / reperfusion injuries, belonging to the technical field of drug preparation. A cerebral ischemia / reperfusion injury model is prepared and curcumin-PVP (polyvinylpyrrolidone) solid dispersions are used for treating the disease model after being dissolved with the saline for injection. Results show that curcumin can obviously improve the injuries of vital organs such as distant livers and kidneys caused by cerebral tissue injuries and cerebral ischemia / reperfusion injuries and can control the expression levels of several factors related to apoptosis, inflammations and oxidation, control further aggravation of the injuries and protect the vital organs such as cerebral tissues and livers and kidneys.

Description

technical field [0001] The invention belongs to the technical field of medicine preparation, and in particular relates to the application of curcumin in the preparation of medicine for treating cerebral ischemia-reperfusion injury. Background technique [0002] Cerebral ischemia is one of the three major diseases that seriously endanger human life and health. It is a common disease of middle-aged and elderly people. . In recent years, its onset tends to be younger, and its incidence rate is gradually increasing. It is the first cause of disability. Among the survivors, 3 / 4 patients lose their ability to work to varying degrees, and moderately disabled patients account for more than 40%. Neuronal degeneration, necrosis, delayed neuronal death or apoptosis may occur in cerebral infarction, and there is no effective treatment at present. [0003] The main pathological changes of infarction after cerebral ischemia are energy deficiency, cerebral edema, inflammation, free radic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/12A61P9/10A61P25/00A61P1/16A61P13/12
Inventor 颜光涛廖杰邓子辉薛辉
Owner GENERAL HOSPITAL OF PLA
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