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Method for quickly detecting biogenic amine

A technology for biogenic amines to be detected, applied in the field of chromatographic analysis, can solve the problems of complex operation, long time-consuming instrument analysis methods, high cost, etc., and achieve the effect of rapid development and convenient and fast semi-quantitative detection

Inactive Publication Date: 2012-08-29
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the qualitative and quantitative analysis methods of biogenic amines have reached a certain level with the progress of analytical instruments in terms of accuracy and precision, the instrumental analysis methods are time-consuming, complicated to operate, and expensive

Method used

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  • Method for quickly detecting biogenic amine
  • Method for quickly detecting biogenic amine

Examples

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Effect test

Embodiment 1

[0023] Prepare putrescine solutions with mass concentrations of 5mg / L, 10mg / L, 15mg / L, 20mg / L, and 25mg / L with 0.1M hydrochloric acid, take 1mL each of the standard solution and the wine sample to be tested, and adjust the pH with 2M NaOH When the value reaches 9.4-10.0, add 1 mL of dansyl chloride derivative (5 mg / L acetone), shake and mix. Place it at 60°C for 30 minutes (shake 3-8 times during the period).

[0024] Use a micro-spotter to spot 10 μL of a sample on a 10cm×10cm chromatographic material polyamide film, place the polyamide sheet that has been blown-dried after spotting with the smooth side outward, and the polyamide side inward, tie it with a rubber band, and put it vertically into the airtight Develop upward in a developing cylinder filled with a saturated developing agent (xylene: glacial acetic acid = 10:1), and the equilibrium solvent in the developing cylinder is obtained by mixing and standing the developing agent in the closed developing cylinder. Chroma...

Embodiment 2

[0026] Prepare histamine solutions with mass concentrations of 5mg / L, 10mg / L, 15mg / L, 20mg / L, and 25mg / L with 0.1M hydrochloric acid, take 1mL each of the standard solution and the beer sample to be tested, and adjust the pH with 2M NaOH value to 9.4, add 1mL dansyl chloride derivative (5mg / L acetone), shake and mix. Placed at 65°C for 25 minutes (shaking 5 times during the period).

[0027] Use a micro-spotter to spot 5 μL of a sample on a 10cm×10cm chromatographic material polyamide film, place the smooth side of the blow-dried polyamide sheet after spotting, and the polyamide side inward, tie it with a rubber band, and put it vertically into the airtight Develop upward in a developing cylinder equipped with a saturated developing agent (xylene: glacial acetic acid = 10: 1), stop chromatography when the leading edge of the developing agent is 0.8 cm away from the top of the film, evaporate the solvent, and display under a 254 nm ultraviolet detection lamp. color. The unfol...

Embodiment 3

[0029] Use 0.1M hydrochloric acid to prepare tyramine solutions with mass concentrations of 5mg / L, 10mg / L, 15mg / L, 20mg / L, and 25mg / L, take 1mL each of the standard solution and the liquor sample to be tested, and adjust the pH with 2M NaOH value to 10.0, add 1mL dansyl chloride derivative (5mg / L acetone), shake and mix. Placed at 58°C for 35 minutes (shaking 3 times during the period).

[0030] Use a micro-spotter to spot 12 μL of a sample on a 10cm×10cm chromatographic material polyamide film, place the smooth side of the blow-dried polyamide sheet after spotting, and the polyamide side inward, tie it with a rubber band, and put it vertically into an airtight container. Develop upward in a developing cylinder equipped with a saturated developing agent (xylene: glacial acetic acid = 10: 1), stop chromatography when the front of the developing agent is 1 cm away from the top of the film, evaporate the solvent, and develop color under a 280nm ultraviolet detection lamp . The ...

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Abstract

The invention provides a method for quickly detecting biogenic amine, which comprises the following steps: performing derivatization on a biogenic amine standard sample and a to-be-tested sample by derivating agent and then providing a chromophoric group, applying the sample on a chromatography material polyamide thin film, unfolding by using a developer, stopping chromatography when the front edge of the developer is 0.5 to 1 cm far away from the top of a thin film, developing, and observing the developing results. According to the invention, the polyamide thin film is used as chromatography detecting material for biogenic amine, the material is easy to obtain, the unfolding is quick, an unfolded thin layer plate can be preserved for a long time for recheck, and the quantitative estimation can be performed by using a thin layer scanner; through utilizing the developer with appropriate proportion, a target object and other components can be separated completely. Therefore, the method provided by the invention can be used for quick determination and semiquantitative detection of biogenic amine, and by using the method, the results can be obtained in the whole-process of 1 hour, and the instrument analysis requires longer time.

Description

technical field [0001] The invention belongs to the field of chromatographic analysis, in particular to a method for rapidly detecting biogenic amines. Background technique [0002] Biogenic amines are a class of nitrogen-containing aliphatic or heterocyclic low-molecular compounds, which are found in foods and beverages containing protein or free amino acids. It widely exists in the tissues of various animals and plants, and has important physiological activities. Low-concentration biogenic amines have obvious relaxation or contraction effects on blood vessels and muscles, can inhibit epileptic seizures, have important regulatory effects on mental activity and cerebral cortex, and have different degrees of positive muscle strength and positive frequency effects on the heart. However, when the human body absorbs excessive biogenic amines, it may cause allergic reactions such as headaches, respiratory disturbances, heart palpitations, and changes in blood pressure. [0003]...

Claims

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Application Information

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IPC IPC(8): G01N30/94
Inventor 潘秋红段长青田园黎姗姗
Owner CHINA AGRI UNIV
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