Streptomyces and multi-antibiotics metabolic regulation fermentation process
A technology of metabolic regulation and fermentation process, applied in fermentation, bacteria, biochemical equipment and methods, etc., can solve the problems of long fermentation period, complicated separation and extraction of fermentation liquid, and many intermediate products, so as to reduce production cost and utilize sugar. The effect of increasing the rate and product concentration and lowering the production cost
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example 1
[0023] Inoculate Streptomyces aureus Chromogenes CGMCC No.5756 strain on Gaoshi No. 1 plate medium, culture at 26°C, pick a single colony after 48 hours, pick the bacteria, and insert it into the cone containing the seed liquid medium In a shaped bottle, placed in a shaker, the rotation speed is controlled at 170rpm, and cultivated at 23°C for 42h. The components of the seed liquid medium are: yeast powder 5.5g / L, corn flour 15g / L, soybean meal powder 15g / L, glucose 35g / L, sodium chloride 3.5g / L, adjust the pH with sodium hydroxide to keep it at 7.1. The seed liquid with a volume ratio (seed liquid medium / fermentation medium) of 11% was pipetted into a fermenter for fermentation, and the liquid volume (V / V) of the fermenter was 30%. The fermentation medium components are: yeast powder 5.5g / L, corn flour 15g / L, soybean cake powder 15g / L, glucose 35g / L, sodium chloride 5g / L, calcium carbonate 10g / L, dipotassium hydrogen phosphate 2g / L, soybean oil 15g / L, inorganic salt is CaCl ...
example 2
[0025] Inoculate Streptomyces aureus Chromogenes CGMCC No.5756 strain on Gaoshi No. 1 plate medium, culture at 27°C, pick a single colony after 52 hours, pick the bacteria, and insert it into the cone containing the seed liquid medium In a shaped bottle, placed in a shaker, the speed is controlled at 200rpm, and cultivated at 29°C for 48 hours. The composition of the seed liquid medium is: yeast powder 3.5g / L, corn flour 10g / L, soybean meal powder 26g / L, glucose 27g / L, sodium chloride 5.5g / L, adjust the pH with sodium hydroxide to keep it at 6.2. The seed liquid with a volume ratio (seed liquid culture medium / fermentation medium) of 15% was pipetted into a fermenter for fermentation, and the liquid volume (V / V) of the fermenter was 40%. The fermentation medium components are: yeast powder 3.5g / L, corn flour 10g / L, soybean cake powder 26g / L, glucose 27g / L, sodium chloride 8g / L, calcium carbonate 12g / L, dipotassium hydrogen phosphate 4g / L, soybean oil 10g / L, inorganic salt is F...
example 3
[0027]Inoculate Streptomyces aureus Chromogenes CGMCC No.5756 strain on Gaoshi No. 1 plate medium, culture at 28°C, pick a single colony after 60 hours, pick the bacteria, and insert it into the cone containing the seed liquid medium In a shaped bottle, place it in a shaker, control the speed at 220rpm, and cultivate it at 26°C for 36 hours. The components of the seed liquid medium are: yeast powder 1.7g / L, corn flour 25g / L, soybean meal powder 30g / L, glucose 15g / L, sodium chloride 8g / L, adjust the pH with sodium hydroxide to keep it at 6.8. The seed liquid with a volume ratio (seed liquid culture medium / fermentation medium) of 7% was pipetted into a fermenter for fermentation, and the liquid volume (V / V) of the fermenter was 50%. The fermentation medium components are: yeast powder 1.7g / L, corn flour 25g / L, soybean cake powder 30g / L, glucose 15g / L, sodium chloride 10g / L, calcium carbonate 15g / L, dipotassium hydrogen phosphate 3g / L, soybean oil 6g / L, inorganic salt is MgSO 4...
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