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Construction and application of recombinant Chicken Marek's Disease Virus SC9-1 strain and SC9-2 strain

A chicken Marek's disease, virus technology, applied in the direction of application, virus, antiviral agent, etc.

Active Publication Date: 2012-08-08
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the addition of a Kan in the process of knocking out the second Meq gene r Genes, according to the current biosafety law of genetic engineering products, shall not contain Kan r Genetic Live Viruses for Vaccines

Method used

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  • Construction and application of recombinant Chicken Marek's Disease Virus SC9-1 strain and SC9-2 strain
  • Construction and application of recombinant Chicken Marek's Disease Virus SC9-1 strain and SC9-2 strain
  • Construction and application of recombinant Chicken Marek's Disease Virus SC9-1 strain and SC9-2 strain

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Embodiment Construction

[0014] Knockout of kan used to replace the second meq gene in the BAC cloning plasmid of GX0101 using partial digestion of the 3 FRT sites generated by incomplete induction of flp recombinase expression r Gene, and by comparing a large number of colonies on 2 LB plates containing and not containing kanamycin, screen out the meq gene and kan that can be used as a vaccine. rBAC cloning of genes. In the previously published research (Li Yanpeng Marek's disease virus meq gene deletion strain biological characteristics and immune effect, doctoral dissertation, 2010, Chinese Academy of Agricultural Sciences. And Yanpeng Li, Aijun Sun, Shuai Su, Peng Zhao, Zhizhong Cui, Hongfei Zhu, Deletion of the Meq gene significantly decreases immunosuppression in chickens caused by pathogenic Marek's disease virus. Virology Journal 2011, 8: 2), a recombinant BAC with two meq genes knocked out has been constructed from the BAC cloning plasmid of GX0101 Plasmid pGX0101Δmeq-BAC was cloned.

[001...

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Abstract

The invention relates to a construction and an application of recombinant Chicken Marek's Disease Virus SC9-1 strain and SC9-2 strain. The recombinant virus construction method solves the technical problem that kanr gene cannot be knocked out again by a present method after kanr gene containing flp recognition sites at two ends is continuously used twice to knock out two specific functional genes on the same virus genome. The obtained recombinant virus MDV SC 9-1 strain and MDV SC 9-2 strain are used as production strains of Marek's Disease live vaccine. The prepared vaccine prevents the very virulent or emerging very virulent plus MDV induced chicken Marek's disease. The protective immunity effect of the vaccine is superior to CVI988 / Rispens strain vaccine which is mostly widely used in foreign and domestic markets at present. The antigenicity of the recombinant virus is more similar to that of Chinese epidemic strain than the antigenicity of the similar virus rMd5deltameq which has been published in the United State. The strains provided by the invention will not induce tumor and has no immune suppression effect. Therefore, the strains are more applicable to China.

Description

[0001] Technical field The present invention relates to the construction and application of recombinant chicken Marek's disease virus SC9-1 strain and SC9-2 strain, belonging to the field of molecular biology of veterinary biological products. Background technique [0002] Since the first isolation of Marek's disease virus (MDV) in 1968, the poultry industry has used vaccine immunization to prevent MD for more than 40 years. As the pathogenicity of MDV continues to evolve, MDV The virulence and pathogenicity of CVI gradually increased, so the vaccine used developed from type III HVT monovalent vaccine to type II SB-1 plus HVT bivalent vaccine, and finally had to use the weakened but still tumorigenic type I CVI988 / Rispens vaccine strain. [0003] From the perspective of the prevalence of MDV in chickens, it has changed from weak virus strains (mMDV) to strong virus strains (vMDV), and super strong virus (vvMDV) strains have become more and more common in the past 20 years. A...

Claims

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Application Information

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IPC IPC(8): C12N15/38C12N7/00C12N5/073A61K39/245A61P31/22C12R1/93
CPCC12N2710/16321C12N2710/16362C12N7/045C12N7/04C12N2710/16034A61K39/255C12N5/10A61K39/245C12N2710/16334C12N7/00C12N2710/16062C12N15/62A61K39/12A61K2039/552A61P31/22
Inventor 崔治中苏帅赵鹏
Owner SHANDONG AGRICULTURAL UNIVERSITY
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