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Preparation method for sunflower seed oligopeptide with high F value

An oligopeptide and sunflower technology, applied in the field of deep processing of plants, can solve the problems of long time, destroy the activity of the product, and take a long time to concentrate, and achieve the effects of mild reaction conditions, high product activity and significant physiological effects.

Inactive Publication Date: 2013-12-18
GUANGDONG FOOD IND INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In refining and separation, activated carbon chromatography and gel filtration are often used at home and abroad to remove aromatic amino acids to increase the F value, and then steam heating and concentration. These methods have certain industrialization limitations: chromatography will consume a lot of water. The concentration of peptides in the lotion and water lotion is low (0.1%~0.5%), so it takes a long time to concentrate, not only the concentration cost is high, but also the concentration time is too long, which is easy to destroy the activity of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Weigh 10 grams of sunflower meal protein, add 50 milliliters of water, adjust the pH to 7.5 with sodium hydroxide, add 0.05 grams of alkaline protease, raise the temperature to 40°C, keep for 40 minutes, then continue to heat up the enzymolysis solution to 90°C, keep Inactivate the enzyme for 15 minutes. After inactivating the enzyme, cool the enzymolysis solution to 45°C, adjust the pH to 4 with citric acid, add 0.05 g of actinase, and keep it for 40 minutes, then continue to heat the enzymolysis solution to 90°C, and keep it for 15 minutes. Inactivate the enzyme, centrifuge after inactivating the enzyme, adjust the centrifugation speed to 2000rpm, keep the centrifugation time for 10 minutes, then pour out the centrifugate and select a membrane with a membrane pore size of 0.5 microns for microfiltration to obtain a clear polypeptide solution. The filtrate is further nanofiltered, and the NF-1014S membrane is selected, the molecular weight cut-off is 300, and the operat...

Embodiment 2

[0025] Weigh 50 grams of sunflower meal protein, add 500 milliliters of water, adjust the pH to 7.6 with sodium hydroxide, add 0.1 g of alkaline protease, raise the temperature to 45°C, keep for 60 minutes, then continue to heat up the enzymolysis solution to 90°C, keep Inactivate the enzyme for 15 minutes. After inactivating the enzyme, cool the enzymolysis solution to 50°C, adjust the pH to 4 with citric acid, add 0.1g actinase, and keep it for 120 minutes, then continue to heat the enzymolysis solution to 90°C, and keep it for 15 minutes. Inactivate the enzyme, centrifuge after inactivating the enzyme, adjust the centrifugal speed to 2500rpm, keep the centrifugation time for 15 minutes, then pour out the centrifugate and select a membrane with a membrane pore size of 0.3 microns for microfiltration to obtain a clear polypeptide solution. The filtrate is further nanofiltered, and the NF-1001T membrane is selected, the molecular weight cut-off is 500, and the operating pressur...

Embodiment 3

[0027] Weigh 100 grams of sunflower meal protein, add 2000 milliliters of water, adjust the pH to 8.5 with sodium hydroxide, add 0.5 g of alkaline protease, raise the temperature to 45°C, keep for 45 minutes, then continue to heat up the enzymolysis solution to 90°C, keep Inactivate the enzyme for 15 minutes. After inactivating the enzyme, cool the enzymolysis solution to 45°C, adjust the pH to 4 with citric acid, add 0.5g actinase, and keep it for 45 minutes, then continue to heat the enzymolysis solution to 90°C, and keep it for 15 minutes. Inactivate the enzyme, centrifuge after inactivating the enzyme, adjust the centrifugation speed to 3000rpm, keep the centrifugation time for 15 minutes, then pour out the centrifugate and select a membrane with a membrane pore size of 8 microns for microfiltration to obtain a clear polypeptide solution. The filtrate is further nano-filtered, and the NF-1016S membrane is selected with a molecular weight cut-off of 1000 and an operating pre...

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Abstract

The invention discloses a preparation method for sunflower seed oligopeptide with a high F value. According to the method, sunflower seed meal protein is used as a raw material, multienzyme combined hydrolysis technology is used for enzymatic hydrolysis so as to hydrolyze sunflower seed concentrated protein into polypeptide with aromatic amino acid as its end groups, the polypeptide is hydrolyzed into aromatic amino acid, free amino acid and oligopeptide by using a second enzyme, then desalination and removal of free amino acid and aromatic amino acid are carried out by using microfiltration and nanofiltration two-stage membrane so as to increase the proportion of the F value, and finally desalination, condensation and freeze drying are carried out to obtain sunflower seed oligopeptide with a high F value. The invention has the following technical effects: (1) reaction conditions are mild, and an obtained product has high activity; (2) a preparation method is clean and environment-friendly, produces no pollution and is in accordance with national policies relating to clean production; and (3) a prepared product has substantial physiological effects and is completely applicable to the industry of health products.

Description

technical field [0001] The invention belongs to the technical field of plant deep processing, and in particular relates to a preparation method of a sunflower oligopeptide with high F value. Background technique [0002] The output of sunflower seeds occupies the second place in the world's major vegetable oil resources. The production of sunflower seeds in my country is relatively abundant, and the Northeast is the main producing area of ​​sunflower seeds in my country. The sunflower meal after oil production is a good edible protein resource (protein content is 40~60%), and it does not have the typical toxic ingredients in other vegetable oils, so it is an ideal vegetable protein resource. However, due to the chromophore in sunflower seeds, its direct use in food is limited. Therefore, it is very promising to use sunflower protein as a resource to prepare oligopeptides with significant physiological effects through biotechnology, especially for the preparation of oligope...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34
Inventor 钟细娥李春荣高展炬詹耀才
Owner GUANGDONG FOOD IND INST
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