Biological method for producing resveratrol
A technology of resveratrol and its production method, which is applied in the field of biomedicine, can solve the problems of complex steps, low production capacity, and low yield, and achieve the effects of being beneficial to industrial production, solving source problems, and reducing production costs
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[0023] The technical solutions of the present invention will be further described below in conjunction with specific embodiments. Using JCAT online gene design software, based on published gene sequences, conservatively design and optimize phenylalanine hydroxylase gene (PAH), tyrosine ammonia lyase gene (TAL), 4-coumaroyl-CoA ligase gene (4CL) and resveratrol synthase (RS) gene sequence, and these target genes were fully synthesized, wherein FASTpfu enzyme was purchased from Beijing Quanshijin Biotechnology Co., Ltd., and restriction enzymes were purchased from Fushitai Si Biotechnology (Shenzhen) Co., Ltd.
[0024] Sequence Optimization and Acquisition of Phenylalanine Hydroxylase Gene (PAH)
[0025] Preferably the phenylalanine hydroxylase gene sequence of Pseudomonas stutzeri was codon-optimized using jcat (http: / / www.jcat.de) for use in Escherichia coli BW25113 and Corynebacterium glutamicum For the expression of ATCC21673, the length of the optimized gene is 786bp, the...
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