Rice wine yeast, rhizopus and application thereof
A technology of rhizopus and rice wine koji, which is applied in the field of microorganisms, can solve the problems of low alcohol content and sugar content, low alcohol content, rice wine can not achieve the taste of rice wine, etc., and achieve sweet flavor, strong wine aroma, wine aroma and sweet taste Effect
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Embodiment 1
[0023] Embodiment 1: the screening of rhizopus described in the present invention
[0024] Dissolve commercially available soil koji with penicillin and streptomycin concentration in sterile water of 30U / ml, and dilute it to 10 -2 -10 -5 With multiple concentrations of g / ml, apply the coating plate method on the prepared wort plate, incubate at 28°C, number the colonies according to the spotting time, and check the appearance of the colonies after 48 hours of incubation.
[0025] Since the rhizopus hyphae are similar in color to the matrix, it is not easy to see, so it should be moved to a bright place for observation. After confirmation, use an inoculation needle to excavate a small piece of mycelium and transplant it on another wort plate medium to continue culturing. Select a typical Rhizopus colony from the single-colony plate and transfer it to the plate. After culturing at 28°C for 48 hours, pick a single colony with strong hyphae and no black spores for transfer and mi...
Embodiment 2
[0028] Embodiment 2: utilize rhizopus described in the present invention to prepare rice wine koji
[0029] Under aseptic conditions, the rhizopus described in the present invention is cultivated with commercially available bran medium, and then inoculated in rice flour with a water content of 45% with a thalline amount accounting for 0.02% of the fermentation medium quality, and put into the culture room Cultivate at about 30°C and at a relative humidity of about 70% for 40 hours. In the middle, the process is controlled according to the product temperature, and the moisture in the rice flour medium is dried until the water content of the rice flour medium is about 8%. library.
Embodiment 3
[0030] Embodiment 3: Utilize the comparative test of different rice wine koji to brew rice wine
[0031] Commercially available rhizopus Q303, rhizopus 3.866, rhizopus 3.866+K yeast, rhizopus 3.866+yeast 1308 are prepared into corresponding rice wine koji according to the method of embodiment 2, and the rice wine koji of the present invention prepared with embodiment 2 is in the same Rice wine was brewed under the environment. During the brewing process, the amount of koji and rice flour used in each rice wine was consistent. Finally, various rice wines were tested, and the results are shown in Table 1.
[0032] Table 1 Test results of each rice wine
[0033]
[0034] As can be seen from Table 1, the compared four kinds of rice wine cannot maintain a high level simultaneously in alcohol and content, so that its mouthfeel cannot have both wine taste and sweetness. The rich aroma of the wine and the high level of sugar content make it sweeter and more delicious on the basis ...
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