Preparation method of biological preparation for preventing and controlling vegetable nematode disease
A biological preparation, nematode technology, applied in the fields of botanical equipment and methods, pesticides, biocides, etc., can solve the problems of unstable field use effect, incomplete root-knot nematode control and other problems, achieve bright color, Reduce the demand for pesticides and labor costs, the effect is good
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Embodiment 1
[0027] The invention discloses a preparation method of a biological preparation for preventing and controlling vegetable nematode disease, which is prepared by first cultivating Paecilomyces lilacinus through a culture medium, and then adding chitin to carry out mixed culture after the culture is mature.
[0028] Wherein, firstly, the culture medium is prepared as follows: 5-6 parts by weight of raw beef extract, 3-4 parts of peptone, 100-200 parts of molasses, 10-20 parts of phosphorous acid, and 10-20 parts of potassium hydroxide; Put the above raw materials into a fermentation tank, add water to 1000 parts, adjust the pH value to 6.5-7.5, heat to 110-130°C and keep for 20-28 hours, then lower the temperature to 30-34°C.
[0029] After that, put the Paecilomyces lilacinus into a fermenter, and stir and oxygenate for cultivation. The cultivation temperature of the Paecilomyces lilacinus is 28-32° C., the pH value is 6.5-7.5, and it is cultivated for 5-7 days. Mature culture s...
Embodiment 2
[0031] The invention discloses a preparation method of a biological preparation for preventing and controlling vegetable nematode disease, which is prepared by first cultivating Paecilomyces lilacinus through a culture medium, and then adding chitin to carry out mixed culture after the culture is mature.
[0032] Wherein, at first making described culture medium is: get raw material beef extract 5 kilograms, peptone 3 kilograms, molasses 100 kilograms, phosphorous acid 10 kilograms, potassium hydroxide 10 kilograms; Adjust the pH value to 7, heat to 120°C and keep for 24 hours, then lower the temperature to 32°C.
[0033] Afterwards, the Paecilomyces lilacinus was dropped into a fermenter, and the Paecilomyces lilacinus was dropped into a fermenter, and stirred and aerated with oxygen for cultivation. The cultivation temperature of the Paecilomyces lilacinus was 32° C., and the pH value was 7 , cultivated for 7 days to obtain a mature culture solution, then mixed the chitin wi...
Embodiment 3
[0035] The invention discloses a preparation method of a biological preparation for preventing and controlling vegetable nematode disease, which is prepared by first cultivating Paecilomyces lilacinus through a culture medium, and then adding chitin to carry out mixed culture after the culture is mature.
[0036] Wherein, at first making described culture medium is: get raw material beef extract 6 kilograms, peptone 4 kilograms, molasses 200 kilograms, phosphorous acid 20 kilograms, potassium hydroxide 20 kilograms; Adjust the pH value to 7, heat to 130°C and keep for 28 hours, then lower the temperature to 30°C.
[0037] Afterwards, the Paecilomyces lilacinus was dropped into a fermenter, and the Paecilomyces lilacinus was dropped into a fermenter, and stirred and aerated with oxygen for cultivation. The cultivation temperature of the Paecilomyces lilacinus was 30° C., and the pH value was 7 , cultivated for 5 days to mature culture solution, then mixed chitin and culture sol...
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