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Preparation method of oxidized nano bacteria cellulose

A technology of nano-bacteria and cellulose, applied in the field of biomedical materials and medical devices, can solve the problems of difficult removal of oxidants, low selective oxidation of oxidants, strong side reactions, etc., achieve controllable degradation, promote hemostasis and healing, The effect of high oxidation rate

Active Publication Date: 2012-07-25
北京中杰瑞康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of strong side reactions (serious degradation), low selective oxidation of oxidants, and difficult removal of oxidants in existing oxidation methods.

Method used

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  • Preparation method of oxidized nano bacteria cellulose
  • Preparation method of oxidized nano bacteria cellulose
  • Preparation method of oxidized nano bacteria cellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Step 1, pretreatment of nano bacterial cellulose membrane.

[0028]Take the nano-bacterial cellulose and wash it with clean water several times to remove the medium and impurities on the surface of the nano-bacterial cellulose membrane; then soak the nano-bacterial cellulose membrane in 0.1mol / L NaOH solution, boil it at 85°C for 90min, and wash out the nano-bacteria Bacteria and residual culture medium in the cellulose membrane; then soak the nano-bacterial cellulose membrane in 0.05mol / L NaOH solution, boil at 85°C for 60min, and then rinse with distilled water several times;

[0029] Step 2, purification of nano bacterial cellulose membrane.

[0030] The above-mentioned pretreated nano-bacterial cellulose membrane is sterilized by ultraviolet light to meet the sterilization requirements of the process, and the subsequent process requires aseptic operation; soak the nano-bacterial cellulose film after the above-mentioned disinfection with distilled water for 3-5 times...

Embodiment 2

[0035] Step 1, pretreatment of nano bacterial cellulose membrane.

[0036] Take the nano-bacterial cellulose and wash it with clean water several times to remove the medium and impurities on the surface of the nano-bacterial cellulose membrane; then soak the nano-bacterial cellulose membrane in 0.1mol / L NaOH solution, boil it at 90°C for 90min, and wash out the nano-bacteria Bacteria and residual culture medium in the cellulose film; then soak the nano bacterial cellulose film in 0.05mol / L NaOH solution, boil at 90°C for 60min, and then rinse with distilled water several times;

[0037] Step 2, purification of nano bacterial cellulose membrane.

[0038] The above-mentioned pretreated nano-bacterial cellulose membrane is sterilized by ultraviolet light to meet the sterilization requirements of the process, and the subsequent process requires aseptic operation; soak the nano-bacterial cellulose film after the above-mentioned disinfection with distilled water for 3-5 times, and u...

Embodiment 3

[0043] Step 1, pretreatment of nano bacterial cellulose membrane.

[0044] Take the nano-bacterial cellulose membrane and rinse it with clean water several times to remove the culture medium and impurities on the surface of the nano-bacterial cellulose membrane; then soak the nano-bacterial cellulose membrane in 0.1mol / L NaOH solution, boil it at 95°C for 90 minutes, and wash out the nano-bacterial cellulose membrane. Bacterial cells and residual medium in the bacterial cellulose membrane; then soak the nano-bacterial cellulose membrane in 0.05mol / L NaOH solution, boil at 95°C for 60min, and then rinse with distilled water several times;

[0045] Step 2, purification of nano bacterial cellulose membrane.

[0046] The above-mentioned pretreated nano-bacterial cellulose membrane is sterilized by ultraviolet light to meet the sterilization requirements of the process, and the subsequent process requires aseptic operation; soak the nano-bacterial cellulose film after the above-men...

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Abstract

The invention relates to a preparation method of oxidized nano bacteria cellulose, belonging to the related fields of biomedical materials and medical appliances. The method comprises the following steps: performing pretreatment and purification treatment on nano bacteria cellulose film to obtain purified nano bacteria cellulose, and shearing into wafers or square slices of different specifications; placing sheared nano bacteria cellulose in a clean wide-necked bottle or a wide-necked bottle holding a nitric acid solution with appointed concentration, and introducing NO2 / N2O4 gas generated by reaction between copper powder and concentrated nitric acid to the wide-necked bottle so as to oxidize the nano bacteria cellulose; and taking out the sample after appointed time, and repeatedly cleaning with deionized water to obtain oxidized nano bacteria cellulose containing carboxyl group on C6 position. The prepared oxidized nano bacteria cellulose has excellent degradation property, biocompatibility and cellular affinity, excellent bleeding arresting performance and mechanical properties, and excellent water permeation, gas permeation, guiding and moisture absorption effects, and has higher application value in the aspects of implantation materials and wound bleeding arresting materials.

Description

technical field [0001] The invention belongs to the related fields of biomedical materials and medical devices, and in particular relates to two methods and uses of the same oxidation system for preparing oxidized bacterial cellulose. Background technique [0002] Bacterial cellulose (BC), also known as microbial cellulose, is a natural biopolymer, which is a kind of unbranched macromolecules directly connected by β-D-glucose through β-1,4-glycosidic bonds. chain polymer. BC is bioactive, biodegradable, bioadaptable, has high crystallinity, high purity, high water holding capacity, ultrafine nanofiber network, high air permeability, high water permeability, high wet strength, high tensile strength and elasticity Modulus and many other unique physical, chemical and mechanical properties. The excellent properties of bacterial cellulose make it have a very broad application prospect in the field of biomedical materials. Since 1987, nearly 10 medical units for skin injuries h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/12C08L1/02A61L15/28A61L27/20
Inventor 郑裕东崔秋艳吴健彭帅栾家斌
Owner 北京中杰瑞康科技有限公司
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