Application of lychee seed flavonoids compounds in terms of preparation of medicine for treating herpes simplex virus encephalitis

A technology of flavonoids and herpes simplex virus, applied in the field of medicine, can solve the problem that the therapeutic effect of herpetic encephalitis has not yet been reported, and achieve the effects of good therapeutic effect, accelerated clearance and improved survival rate

Inactive Publication Date: 2012-07-25
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Litchi kernel flavonoids not only have various effects such as lowering blood sugar, regulating blood fat and antioxidation, but also have antiviral effects. It has been reported in the literature that they have anti-hepatitis B virus (HBV), influenza virus (IFV), Respiratory syncytial virus (RSV), SARS virus and other viruses, we also found that it has an anti-herpes simplex virus effect in vitro, but its therapeutic effect on herpes encephalitis caused by herpes simplex virus infection has not yet been established. see the report

Method used

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  • Application of lychee seed flavonoids compounds in terms of preparation of medicine for treating herpes simplex virus encephalitis
  • Application of lychee seed flavonoids compounds in terms of preparation of medicine for treating herpes simplex virus encephalitis
  • Application of lychee seed flavonoids compounds in terms of preparation of medicine for treating herpes simplex virus encephalitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Extraction and Purification of Litchi Kernel Flavonoids

[0017] Break the litchi core into powder and pass through a 40-mesh sieve, weigh a certain amount of litchi core powder, add a corresponding volume of ethanol solution with a concentration of 60vol% according to the ratio of solid to liquid 1:12, and then extract it under a microwave frequency of 700W for 150S. And shake intermittently, after two extractions, filter the extract, add twice the amount of absolute ethanol to stand for a period of time after concentration, fully precipitate and filter to remove macromolecular impurities such as protein. Ethanol is recovered under reduced pressure, concentrated and then transferred to the water phase to prepare litchi seed extract for resin adsorption. Add 600mg of pretreated X-5 adsorption resin and 450ml of the litchi core extract prepared above into a ground-mouthed conical flask with a stopper, adjust the mass concentration of the sample solution to 0.5...

Embodiment 2

[0018] Example 2: Qualitative identification and content determination of flavonoids in litchi core extract

[0019] 1) First take litchi core extract, dissolve in 60vol% ethanol, mix with HCl-Mg, AlCl 3 , Pb(Ac) 3 The reactions were all positive, indicating that flavonoids were contained in the litchi core extract.

[0020] 2) With 60vol% ethanol as solvent, with NaNO 2 -Al(NO 3 ) 3 -NaOH is used as a chromogenic agent, respectively as the absorption curves of rutin standard substance and litchi kernel extract, both of which have a strong absorption peak at 508nm, so 508nm is selected as the measurement wavelength. Accurately weigh 5.00 mg of rutin standard substance, dissolve it with 50 vol% ethanol and dilute it to a 50 ml volumetric flask to prepare a rutin standard solution (100 μg / ml). Accurately measure 0.00, 1.00, 2.00, 3.00, 4.00, 5.0, 6.0, and 7.0ml of rutin standard solution and place them in 25ml volumetric flasks, add 1ml of 5wt% sodium nitrite solution and s...

Embodiment 3

[0021] Embodiment 3: Toxic effect of litchi kernel flavonoids on mice

[0022] Take 40 Kunming mice (male), 4-5 weeks old, 16±1g. They were randomly divided into 5 groups with 8 rats in each group, including a normal control group. The lychee kernel flavonoids were diluted with double-distilled water into 4 groups of 3436.25, 344.00, 34.40, and 3.44mg / kg. After the mice were fasted for 4 hours, each mouse was given 0.2ml orally, once a day, for 14 consecutive days. Feed and water were supplemented regularly, and litter was replaced. The indoor temperature was 22±2°C, and the changes in animal weight and death were recorded. After 14 days of observation, it was found that the animals in three different concentration groups of 344.00mg / kg, 34.40mg / kg, and 3.44mg / kg had no poisoning and death. The safe administration concentration is below 344.00mg / kg concentration.

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Abstract

The invention discloses application of lychee seed flavonoids compounds in terms of preparation of a medicine for treating virus encephalitis caused by infection of herpes simplex virus (HSV). The application specifically embodies that the lychee seed flavonoids compounds obviously suppress the HSV in mice during in-vivo test, the infection symptom of the mice is relieved along with increase of concentration of the medicine, mortality of the mice is reduced, mean survival time of the mice is prolonged, encephalitis symptom of the mice is obviously relieved, the mice are remarkably different from mice in a virus control group, and the curing effect is positively correlated with the concentration of the medicine.

Description

technical field [0001] The invention relates to the use of litchi kernel flavonoids in the preparation of medicines for treating herpes simplex virus encephalitis, belonging to the technical field of medicine. Background technique [0002] Herpes simplex virus (HSV) infection in the population is very common, and more serious is that HSV infection can cause herpes simplex virus encephalitis (HSVE), also known as acute necrotizing encephalitis, the case fatality rate is as high as 50%-70%, and the survival rate is as high as 50%. Patients with high disability rate, often have varying degrees of brain dysfunction, serious sequelae. At present, antiviral drugs such as Herpes, Vidarabine, and Acyclovir are mainly used in the clinical treatment of HSV infection, but these drugs have relatively large side effects, such as obvious gastrointestinal symptoms, allergies and drug resistance. Therefore, the research and development of new anti-herpes simplex virus drugs has important p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/77A61P31/22A61K131/00
Inventor 侯炜罗凡朱丹杨艳彭璇李立
Owner WUHAN UNIV
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