Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Hypericin albumin nanoparticle-immune globulin G antibody conjugate and preparation method thereof

A technology of albumin nanoparticles and immunoglobulins, which is applied in the direction of antiviral agents, antitumor drugs, drug combinations, etc., to achieve the effects of enhanced targeting, low energy consumption, and easy absorption

Active Publication Date: 2012-07-18
GUANGDONG HINAPHARM PHARMA CO LTD
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above only improves the effective content of hypericin to a certain extent, but if the effective utilization rate of hypericin is further enhanced, there is no relevant record in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hypericin albumin nanoparticle-immune globulin G antibody conjugate and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Dissolve 200 μL immunoglobulin G antibody and 200 μL hypericin albumin nanoparticles (mass ratio 1:1) in 4 ml PBS buffer, at room temperature, the stirring condition is 400 rpm / min; at 0.5 ml / min slowly Add 100 μL of fresh glutaraldehyde dropwise (the so-called fresh glutaraldehyde means that it is ready-to-use), stir well for 2 hours to cross-link hypericin albumin nanoparticles with immunoglobulin G antibody, and freeze-dry to obtain Hypericum Albumin Nanoparticles-Immunoglobulin G Antibody Conjugate.

[0031] Oral administration of ordinary hypericin, hypericin albumin nanoparticles and hypericin albumin nanoparticle-immunoglobulin G antibody conjugates to immunosuppressed mice, and the determination of immune organ indexes (including spleen index and thymus index). From the statistical results of the spleen index, it can be seen that the spleen index of the blank control group was (3.60±0.89 mg / g), the spleen index of the immunosuppressive model group was (2.16±0.4...

Embodiment 2

[0033]Dissolve 150 μL immunoglobulin G antibody and 300 μL hypericin albumin nanoparticles (mass ratio 1:2) in 4.5 ml PBS buffer, the pH of the buffer is 7.2. At -10°C, under the stirring condition of 400 rpm / min, slowly add 75 μL of fresh glutaraldehyde dropwise at 0.5 ml / min, stir well for 2 h to cross-link hypericin albumin nanoparticles with immunoglobulin G antibody, freeze After drying, the hypericin albumin nanoparticle-immunoglobulin G antibody conjugate is obtained. Oral administration of ordinary hypericin, hypericin albumin nanoparticles and hypericin albumin nanoparticle-immunoglobulin G antibody conjugates to immunosuppressed mice, and the determination of immune organ indexes (including spleen index and thymus index). From the statistical results of the spleen index, it can be seen that the spleen index of the blank control group was (3.60±0.89 mg / g), the spleen index of the immunosuppressive model group was (2.16±0.45 mg / g), and the normal hypericin group Comp...

Embodiment 3

[0035] Dissolve 100 μL immunoglobulin G antibody and 300 μL hypericin albumin nanoparticles (mass ratio 1:3) in 4 ml PBS buffer, at 10°C, stirring at 700 rpm / min, at 0.5 ml / min Slowly add 50 μL of fresh glutaraldehyde (the so-called fresh glutaraldehyde refers to the ready-to-use), stir well for 2 h to cross-link hypericin albumin nanoparticles with immunoglobulin G antibody, and freeze-dry to obtain gold Sericin albumin nanoparticles-immunoglobulin G antibody conjugate. Oral administration of ordinary hypericin, hypericin albumin nanoparticles and hypericin albumin nanoparticle-immunoglobulin G antibody conjugates to immunosuppressed mice, and the determination of immune organ indexes (including spleen index and thymus index). From the statistical results of the spleen index, it can be seen that the spleen index of the blank control group was (3.60±0.89 mg / g), the spleen index of the immunosuppressive model group was (2.16±0.45 mg / g), and the normal hypericin group Compared...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
molecular weightaaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a hypericin albumin nanoparticle-immune globulin G antibody conjugate, which comprises the following preparing steps: step (1), dissolving the hypericin albumin nanoparticle and the immune globulin G antibody conjugate into phosphate buffer solution (PBF), step (2), adding glutaraldehyde into the solution obtained by the step (1), and obtaining the hypericin albumin nanoparticle-immune globulin G antibody conjugate. The hypericin albumin nanoparticle-immune globulin G antibody conjugate has the advantages of high effective utilization; simultaneously, the preparation method of the hypericin albumin nanoparticle-immune globulin G antibody conjugate has the advantages of being simple in process, easy to operate and specially suitable for industrial production.

Description

technical field [0001] The invention relates to an antiviral natural medicine, in particular to a hypericin albumin nanoparticle-immunoglobulin G antibody conjugate. Background technique [0002] Hypericin (HY) is a naphthalene dianthrone compound extracted from Hypericum perforatum Linn., a plant of the Clusiaceae family. It has photosensitizing activity and photosynthetic activity, and can be applied to the photochemical diagnosis of tumors and treatment. However, due to the instability of hypericin to light and heat, the content of active ingredients of hypericin in the market is extremely low, which is far from meeting the requirements of terminal product development. Nano-drugs refer to the drugs made of nano-scale polymer nanoparticles (nanoparticles, NP), nanospheres (nanospheres, NS), nanocapsules (nanocapsules, NC), etc., combined with drugs in a certain way. Its particle size may exceed 100 nm, but generally should be less than 500 nm. Nano-drugs can also be nano...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K31/122A61K47/42A61P37/04A61P31/12A61P35/00
Inventor 梁剑平胡小艳陶蕾王学红刘宇尚若峰赫宝成华兰英
Owner GUANGDONG HINAPHARM PHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com