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Extract method of cordyceps militaris adenosine

A technology of Cordyceps militaris adenosine and extraction method, which is applied in the field of extraction of Cordyceps militaris adenosine, can solve the problems that the active ingredients of Cordyceps militaris cannot be completely extracted, it is difficult to break the cell wall of Cordyceps militaris, and the loss of active ingredients is large, so as to reduce the burden on the stomach, Easy separation and purification, quality control effect

Active Publication Date: 2012-07-11
贵州贵安精准医学股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Conventional extraction methods, such as water extraction, water extraction and alcohol precipitation, alcohol extraction, etc., have a single extraction process and cannot completely extract the active ingredients in Cordyceps militaris
At the same time, these extraction methods still have the disadvantages of insufficient extraction, large loss of active ingredients, many processes, long cycle, and high cost in terms of retaining active ingredients and discarding ineffective ingredients.
And the conventional extraction method is difficult to break the hard cell wall of Cordyceps militaris, brings great difficulty to the extraction process, causes very big waste and loss

Method used

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  • Extract method of cordyceps militaris adenosine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: the cultivation of Cordyceps militaris

[0037] Silkworm chrysalis is used as the main raw material, rice is used as auxiliary material, phosphate and water are added to make medium to cultivate Cordyceps militaris.

[0038] a. The configuration of culture medium: Cordyceps militaris culture medium of the present invention is aqueous solution, is made of the composition of following concentration:

[0039] Dried silkworm chrysalis powder 140 g / L;

[0040] Rice flour 60 g / L;

[0041] Potassium dihydrogen phosphate 1.2 g / L;

[0042] Sodium dihydrogen phosphate 1.2 g / L;

[0043] Preferred specific preparation method is as follows:

[0044] Accurately weigh 2.1kg of dried silkworm chrysalis powder, 900g of rice flour, 18g of potassium dihydrogen phosphate, and 18g of sodium dihydrogen phosphate, add 12 L of purified water, and dissolve the KH 2 PO 4 , NaH 2 PO 4 , After fully mixing to obtain a medium solution with a volume of 15L, add the above-mentio...

Embodiment 2

[0055] Embodiment 2: the low-temperature ultrafine pulverization of Cordyceps militaris

[0056] Weigh 150 g of dried fruiting bodies of Cordyceps militaris each time, put them into a superfine pulverizer, and pulverize them for 6 minutes. During the whole crushing process, water at 4-10°C is used as cooling liquid to ensure the biological activity of the active ingredients. After crushing, samples were taken to detect the crushing effect with a scanning electron microscope. The effects of ordinary crushing and superfine crushing are shown in figure 1 and figure 2 . By comparison figure 1 and figure 2 It can be clearly seen that the cell wall of the fruiting body of Cordyceps militaris is intact after ordinary crushing, and the cells still gather in clumps. The cell wall after superfine pulverization is incomplete, and the contents are faintly visible, so the superfine pulverization of Cordyceps militaris spores is completely broken, and the nutrients are effectively re...

Embodiment 3

[0057] Embodiment 3: repeated freeze-thaw extraction

[0058] Weigh 100g ± 0.1g of Cordyceps militaris superfine powder, add 10 times the volume of ultrapure water 1000ml to the Cordyceps militaris superfine powder, mix well and freeze in a refrigerator at -80°C for 4 hours to make the cells Rapidly form large ice crystals to fully destroy the cell structure; after freezing, thaw quickly in a water bath at 37°C to strengthen the degree of cell damage. So repeated freezing and thawing 5 times. After repeated freezing and thawing, centrifuge at a centrifugal force of 10000 g for 20 minutes, and collect the supernatant. Continue the extraction after the precipitation is naturally air-dried.

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Abstract

The invention relates to an extract method of cordyceps militaris adenosine and belongs to the technical field of biological product processing. The extract method provided by the invention mainly comprises the following steps: superfine pulverizing cordyceps militaris fruiting body under low temperature, then repeating freezing and thawing for 3 to 7 times in order to destroy the internal components of the cellular interior, and further damaging the cell wall of cordyceps militaris, simulating the human gastrointestinal tract acid-base environment, extracting adenosine in cordyceps militaris by microwave heating using bio-mimetic extract technology for three times, finally measuring the content of adenosine in the extract using high performance liquid chromatography. In the invention, human gastrointestinal tract acid-base environment is simulated to extract cordyceps militaris adenosine, acid-base environment is not only favor of forming a salt from adenosine and dissolving, but also the obtained adenosine has a high extraction rate, which is easy to proceed subsequent separation and purification process, also easy to be proceeded into various types of medicament. The invention ensures the active ingredient of cordyceps militaris is absorbed directly, and the gastrointestinal burden is reduced.

Description

technical field [0001] The invention relates to a method for extracting adenosine from Cordyceps militaris, belonging to the technical field of biological product processing. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, Cordyceps militaris, Cordyceps militaris, etc., belongs to the fungal phylum, Ascomycota subphylum, Sclerotinia, Spheroides, Ergotaceae, Cordyceps genus in taxonomy. It is a traditional Chinese medicine in China and has great medicinal value. [0003] Cordyceps militaris contains a variety of chemical components and has a variety of medicinal effects. It is similar to the pharmacological function and clinical effect of Cordyceps sinensis, but the price is far lower than that of Cordyceps sinensis. Therefore, Cordyceps militaris has gradually become a substitute for Cordyceps sinensis and has great potential market. [0004] Conventional extraction methods, such as water extraction, water extraction and alcohol precip...

Claims

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Application Information

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IPC IPC(8): C07H19/167C07H1/08
Inventor 卢丽丽陈剑清舒特俊张耀洲
Owner 贵州贵安精准医学股份有限公司
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