Preparation method of metal nanoparticle dimer

A metal nanoparticle, dimer technology, applied in nanostructure manufacturing, biochemical equipment and methods, nanotechnology, etc., can solve the problems of low assembly yield, complicated purification steps, etc., and achieve easily controllable conditions and simple operation. , good repeatability

Active Publication Date: 2012-07-11
SUZHOU NAFANG TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to propose a method for preparing a high-purity metal nanoparticle dimer, to overcome the shortcomings of existing solutions such as low assembly yield and cumbersome purification steps.

Method used

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  • Preparation method of metal nanoparticle dimer
  • Preparation method of metal nanoparticle dimer
  • Preparation method of metal nanoparticle dimer

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1: In the aqueous solution containing 70% acetonitrile, add the active intermediate DHLA-NHS and the single-stranded primer P30 and P30C (P30 and P30C are the oligonucleotides that contain 30 bases, and sequence complementary ), and adding a small amount of triethylamine as an acid-binding agent, reacted for 12 hours. The DHLA-modified primers P30 and P30C were concentrated by vacuum evaporation equipment, and the acetonitrile component was evaporated.

[0032] To prepare 13nm symmetric gold nanoparticle dimers connected by 30bp dsDNA, DHLA-P30 and DHLA-P30C were paired and hybridized into double strands in 1×PBS buffer from 95°C to room temperature under equimolar conditions For dsP30, an equimolar amount of dsP30 was added to a 13 nm gold nanoparticle aqueous solution containing 50 mM NaCl. React for 6 hours. Subsequently, excessive NaCl was added to cause the aggregation and sedimentation of gold nanoparticles, further promoting the coupling of gold nano...

Embodiment 2

[0034] Example 2: Add active intermediate DHLA-NHS and amino-modified single-stranded primers P15 and P15C in an aqueous solution containing 70% acetonitrile (P15 and P15C are oligonucleotides containing 15 bases, and the sequences are complementary ), and adding a small amount of triethylamine as an acid-binding agent, reacted for 12 hours. The DHLA-modified primers P15 and P15C were concentrated by vacuum evaporation equipment, and the acetonitrile component was evaporated.

[0035] Prepare 13nm symmetric gold nanoparticle dimers connected by 15bp dsDNA. Under equimolar conditions, DHLA-P15 and DHLA-P15C are paired and hybridized into double strands in 1×PBS buffer from 95°C to room temperature slowly For dsP15, an equimolar amount of dsP15 was added to a 13 nm gold nanoparticle aqueous solution containing 50 mM NaCl. React for 6 hours. Then add excess NaCl to make the gold nanoparticles agglomerate and settle, and further promote the coupling of gold nanoparticles into di...

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Abstract

The invention relates to a method for preparing a high-purity pitch-adjustable metal nanoparticle dimer at high yield. The method is characterized in that two length-adjustable double-stranded DNA molecules respectively modified by a dual-thiol molecule at the tail ends are used as the connection part; Au (gold) nanoparticles are assembled to a discrete dimer through the chemical bond formed between the thiol and the surface of the Au nanoparticles; and the discrete dimer is separated by one-step electrophoresis method to obtain the high-purity target product. The method provided by the invention can be also used for preparing high-purity Au nanoparticle dimer with symmetric and asymmetric compositions. The method has the advantages of less steps, simple operation, easily-controlled conditions, and good repeatability, and solves the problems of single-DNA-modified Au nanoparticles, such as low assembly purity and complicated steps. The method can provide a firm platform for the basic research of plasmon resonance photonics, and can be used for molecular spectrum studies, such as surface-enhanced Raman scattering and metal-enhanced fluorescence. The prepared high-purity pitch-adjustable metal nanoparticle dimer can be used as an ultrahigh-sensitivity sensor based on plasmon resonance.

Description

technical field [0001] The invention relates to a method for programmably assembling discrete metal nanoparticle plasmon resonance dimers by using biomacromolecular DNA, and belongs to the field of nanomaterial preparation. Background technique [0002] When the incident light of a specific wavelength interacts with the free electrons on the surface of the metal nanoparticles, the collective oscillation of the free electrons can be excited, and the light is confined to the nanoscale on the surface of the metal nanoparticles, that is, localized surface plasmon resonance is generated. Noble metal nanoparticles have unique optoelectronic properties due to the localized surface plasmon resonance effect, such as a significant electromagnetic field enhancement phenomenon in the near-field region of the particle surface, which makes the molecular Raman scattering signal there enhanced by several to a dozen order of magnitude, so as to realize single-molecule Raman spectroscopy dete...

Claims

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Application Information

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IPC IPC(8): B82B3/00B82Y40/00C12Q1/68
Inventor 王强斌兰祥
Owner SUZHOU NAFANG TECH DEV
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