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Nostoc capable of degrading polychlorinated biphenyls and application thereof

A technology of polychlorinated biphenyls and Nostoc algae, applied in the fields of application, algae products, botany equipment and methods, etc., can solve problems such as expensive, no literature reports on polychlorinated biphenyls, secondary pollution prices, etc.

Inactive Publication Date: 2012-07-11
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above physical or chemical methods have the problem of secondary pollution or expensive
Although domestic and foreign scholars have conducted research on the degradation of PCBs by microorganisms, there is no literature report on the degradation of PCBs by algae species in my country.

Method used

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  • Nostoc capable of degrading polychlorinated biphenyls and application thereof
  • Nostoc capable of degrading polychlorinated biphenyls and application thereof
  • Nostoc capable of degrading polychlorinated biphenyls and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Separation and screening of algal species

[0023] Soil sample: collected from a PCB-contaminated rice field in Taizhou City, Zhejiang Province.

[0024] Enrichment and separation of algal species: screened with liquid medium containing 10ppmPCBs.

[0025] The culture medium of the present invention is: BG11 inorganic salt culture medium (1000mL), and its composition is shown in Table 1.

[0026] Table 1: Composition of BG11 Inorganic Salt Medium

[0027]

[0028] where A 5 The composition of the solution is shown in Table 2.

[0029] Table 2: A 5 Solution composition

[0030] Composition

concentration

Composition

concentration

h 3 BO 3

2.86g / L

MnCl 2

1.81g / L

ZnSO 4 ·7H 2 o

0.222g / L

CuSO 4 ·5H 2 o

0.079g / L

NaMoO 4 2H 2 o

0.39g / L

Co(NH 3 ) 2 ·6H 2 o

0.39g / L

[0031] (2) Molecular identification of algal species

[0032] The 16SrDNA sequence of Nostoc...

Embodiment 2

[0039] Example 2: PCC7120 algae biodegradation 2mg / L PCB1254 kinetic experiment

[0040] Nostoc sp.PCC7120 algae were placed in Erlenmeyer flasks at 25°C under 2000lux light and cultivated until logarithmic growth phase. Add 20mL of BG11 inorganic salt medium to each Erlenmeyer flask, and add methanol dissolved in each Erlenmeyer flask. The initial concentration of PCB1254 working solution is 2mg / L. Samples were taken according to the culture time of 2 days, 4 days, 6 days, and 8 days, and the content of PCB1254 in the culture medium was determined by GC-ECD detector to study its degradation kinetics. Degradation results see figure 1 .

[0041] As a result, it is found that the degradation efficiency of the algae species of the present invention to 2mg / L PCB12548 days is 46.98%, and its degradation reaction kinetic equation is: C t =29.795e -0.0614t (R 2 =0.974), the degradation rate was 1.25mg·L -1 d -1 , compared with the results of the control group, there is a signi...

Embodiment 3

[0042] Example 3: Experimental results of PCC7120 algae biodegrading 2mg / L of PCB125425 days

[0043] Nostoc sp.PCC7120 algae were cultured in Erlenmeyer flasks at 25°C and 2000lux light to logarithmic growth phase, and 20 mL of BG11 inorganic salt medium was added to each Erlenmeyer flask, and methanol dissolved in each Erlenmeyer flask was added. The PCB1254 working solution was taken at an initial concentration of 2 mg / L, and samples were taken at 25 days of culture time. The content of PCB1254 in the degraded medium was measured with a GC-ECD detector, and the medium without algae liquid was set as the blank control group. The degradation results are shown in Table 4.

[0044] Table 4: Degradation effect of Nostoc sp.PCC7120 Nostoc on PCB1254

[0045]

[0046]Table 4 illustrates the experimental results of algal species of the present invention degrading 2mg / L PCB125425 days under laboratory conditions. It can be seen from the table that the remaining PCB1254 in the re...

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Abstract

The invention provides nostoc capable of degrading polychlorinated biphenyls. A frond of the nostoc is multicellular protonema, has algal filaments and a colloid sheath, and can fix inorganic nitrogen. The nostoc can degrade polychlorinated biphenyl (PCB) 1254 efficiently; after the nostoc is exposed for a week, the degradation efficiency of the nostoc on the PCB1254 is over 60 percent, and the content of residual chlorine ions tested in a culture medium after the degradation and dechloridation of the PCB1254 is 2.26mg / L and 3.01mg / L; the one-month degradation efficiency of the nostoc on six typical indicative polychlorinated biphenyl (PCB52, PCB101, PCB118, PCB138, PCB137 and PCB180) in environment is more than 70 percent, wherein the degradation rate of the PCB52 is 98.7 percent; and after the PCB1254 is degraded for one month, the residual amount of the PCB1254 in the culture medium is only 15.65 percent. Therefore, the nostoc can be widely applied to the degradation of the PCBs insoil environment.

Description

(1) Technical field [0001] The invention relates to a new algal species capable of degrading polychlorinated biphenyls—Nostoc sp. PCC 7120, and its application in microbial degradation of polychlorinated biphenyls (PCBs). (2) Background technology [0002] Polychlorinated biphenyls (PCBs) are polychlorinated compounds in which the hydrogen on the biphenyl ring is replaced by chlorine. Due to its stable chemical properties, heat resistance, non-combustibility, easy solubility in oil, and excellent insulation performance, it is widely used as an insulating fluid for power equipment (such as transformers, capacitors), paint, plastics, and additives for carbonless copying paper. [0003] PCBs, as endocrine disruptors, have harmful effects on the reproductive system, nervous system and immune system of animals and humans. PCBs have an inducing effect on skin, liver, reproductive system, and immune system lesions and even cancer, and accumulate in organisms through the food chain...

Claims

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Application Information

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IPC IPC(8): A01H13/00
Inventor 张杭君蒋晓军黄黎明寿晓璐贾秀英胡赐明
Owner HANGZHOU NORMAL UNIVERSITY
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