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Pandora bacterium with dichloromethane degrading capability and application thereof

A technology of dichloromethane and Pandora bacteria, applied in the direction of bacteria, microorganism-based methods, water/sludge/sewage treatment, etc., can solve the problems of low solubility, limited, containing, etc., and achieve the effect of broad application prospects

Active Publication Date: 2012-07-04
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, scholars at home and abroad have done a lot of research on the biodegradation of alkanes. However, due to the extremely low solubility of DCM and the chlorine atoms in its structure, the DCM-degrading bacteria isolated so far are still relatively limited, mainly including Pseudomonas ), Methylobacterium, Hyphomicrobium, Bacillaceae, Mycobacterium and Fusobacterium, etc., the degradation efficiency of these isolated strains needs to be further improved

Method used

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  • Pandora bacterium with dichloromethane degrading capability and application thereof
  • Pandora bacterium with dichloromethane degrading capability and application thereof
  • Pandora bacterium with dichloromethane degrading capability and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Isolation, purification and identification of Pandoraea pnomenusa flx-1

[0025] 1. Isolation and purification of Pandoraea pnomenusa flx-1

[0026] Pandora sp. flx-1 is a Gram-negative bacterium domesticated and isolated from the activated sludge of a refinery wastewater treatment station in Ningbo, Zhejiang. Specific steps are as follows:

[0027] Activated sludge from a wastewater treatment station of a refinery in Ningbo, Zhejiang. After washing with tap water for five times, air-expose for 48 hours to remove residual organic matter as much as possible. Prepare inorganic salt culture solution (pH7), use DCM as the only carbon source to conduct directional acclimation to activated sludge, replace fresh inorganic salt culture solution every 3 days, measure the pH of the culture solution every day, and the pH drops significantly around 40 days.

[0028] Prepare DCM initial concentration of 50mg / L inorganic salt medium, the preparation method is as follows...

Embodiment 2

[0040] Example 2: Response surface optimization of strain Pandoraeapnomenusa flx-1 (CCTCC NO: M2011242) environmental factors for degradation of DCM

[0041] 1. Response surface test design and acquisition of the prediction model for strain degradation DCM rate

[0042] The effects of three factors including culture solution pH, culture temperature and culture solution salinity on the degradation effect of DCM were investigated, and the SAS system software was used to fit the model of the DCM degradation rate obtained in the experiment, so as to obtain the predictive strain Pandoraea pnomenusa flx-1 Degradation rate of DCM under different culture conditions. The specific implementation steps are as follows:

[0043] Taking pH 7 (Code=0), temperature 30°C (Code=0), and salt concentration 0.5% (Code=0) as the central values, the SAS system software was used to carry out a three-factor and three-level experimental design (Table 2).

[0044] Table 2: Response and prediction valu...

Embodiment 3

[0053] When the pH of the culture solution was 7.3, the culture temperature was 33°C, and the salinity of the culture solution was 0.66%, the actual degradation rates of DCM by the strain Pandoraea pnomenusa flx-1 were 5.37, 5.41 and 5.42 mg / (L·h), and the average degradation rate was 5.40mg / (L·h), close to the predicted value of the model, indicating that this combination of environmental factors is the best combination of environmental factors for strain flx-1 to degrade DCM. Example 3: Detection of the degradation performance of Pandoraeapnomenusa flx-1 to different concentrations of DCM

[0054] Under the optimum environmental factor conditions (culture medium pH 7.3, culture temperature 33°C, culture medium salinity 0.66%), the degradation performance of the strain flx-1 to the initial concentration of 25-2000mg / L DCM was investigated. The results showed that the strain flx-1 could completely degrade 25-1500mg / L DCM, but only partially degrade the initial concentration of...

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Abstract

The invention provides a dichloromethane degrading bacterium, namely a pandoraeapnomenusa strain flx<-1>, and an application thereof. The strain is preserved in China Center for Type Culture Collection in Wuhan University (430072), Wuhan, China, the preservation number is CCTCC NO.: M 2011242, and the preservation date is July 8, 2011. The pandoraeapnomenusa strain flx<-1> is taken from a wastewater treatment unit and has good degrading effects on chlorinated hydrocarbon compounds, in particular on DCM (dichloromethane), and the DCM can be completely converted into CO2, H2O, biologic cells and other harmless substances. Meanwhile, benzene, toluene and other common industrial pollutants can be degraded to certain extent through the strain. Therefore, the strain has broad application prospects in biological purification of industrial exhaust gas and wastewater.

Description

(1) Technical field [0001] The present invention relates to a strain-Pandoraea pnomenusa strain flx-1, and its application in microbial degradation of dichloromethane, chlorobenzene, benzene, toluene or ethylbenzene. (2) Background technology [0002] Dichloromethane (CH 2 Cl 2 , Dichloromethane, referred to as DCM) is a very widely used non-water-soluble organic solvent, which is widely used in degreasing of metal materials, paint removal industry and pharmaceutical industry. With the acceleration of industrialization and urbanization, the demand for DCM in various countries is also increasing. DCM is not only the main pollutant of the atmosphere, but also one of the important components of urban wastewater and industrial sewage. More than 90% of DCM comes from industrial use and waste combustion. Because it is not easily degraded, the half-lives of DCM in the atmosphere and water environment are 70 days and 700 years, respectively. DCM is cumulative and persistent in t...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F3/02B01D53/84B01D53/70B01D53/72C12R1/01C02F101/36C02F101/34C02F101/32
CPCY02A50/20Y02P20/59Y02W10/10
Inventor 陈建孟成卓韦蒋轶锋傅凌霄於建明朱润晔
Owner ZHEJIANG UNIV OF TECH
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