Preparation of water-soluble fluorescence probe and rapid detection of iron ion by use of the same

Abstract

The invention provides a preparation method of a water-soluble fluorescence probe and a rapid detection method of iron ions. The fluorescence probe has very high selectivity and sensitivity on the iron ion in water and serum; when the fluorescence probe is selectively bonded to the iron ion, the solution can be watched through a naked eye that the color is changed from yellow to red brown and the lower limit of the detection can be up to 10<-6>M; and the on-site real-time direct rapid detection can be carried out with no need for instruments. The fluorescence probe can be prepared into an iron ion detecting test paper and a detection kit, and can also be coated and prepared into a portable solid detector by utilizing the viscosity of carbohydrate compounds per se. The concrete structure of the fluorescence probe is as follows (with galactosyl derivative ligand as an example): the glycosyl ligand therein is not only the galactosyl derivative ligand, but can also be all other carbohydrate chemicals and carbohydrate chemical derivatives; and the fluorogen therein is not only 2-hydroxy-1-naphthaldehyde, but can also be other aromatic aldehyde of which the ortho position is a hydroxyl.

Description

technical field [0001] The invention relates to the preparation of a class of water-soluble fluorescent probes and the rapid detection of iron ions. Background technique [0002] Iron ions are essential substances for the manufacture of heme and myoglobin, and for the promotion of vitamin B metabolism. The human body needs iron within a certain range. Many biological and chemical processes at the cellular level require the participation of iron ions. If it is too low, it will cause anemia. If it is too high, the metabolism of trace elements such as zinc and copper will be out of balance in the body, thus affecting the human body. The absorption of trace elements reduces the immunity of the body and makes it susceptible to pathogen infection. Therefore, excessive iron ions in the water will affect human health. Therefore, the study of fluorescent probes that can be used for the detection of iron ions is of great significance to the environment and life sciences. It has been...

AI Technical Summary

Problems solved by technology

It has been reported in the literature that the synthesis method of fluorescent probes for iron ions is complex and costly, and the color change cannot be observed with the naked eye, and expensive detection instruments must be used

In addition, there are still disadvantages such as sample pretreatment, complex detection, poor water solubility, and expensive detection.

Images