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Preparation method for biomimetic matrix type biological wound healing material

A biological and matrix technology, applied in the field of medical biomimetic material preparation, can solve the problems such as matrix-type biomimetic healing materials that have not yet been seen, so as to enhance the ability of cells to resist adverse environments, reduce poor prognosis, and improve biosafety Sexual and Bioactive Effects

Inactive Publication Date: 2014-08-06
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no relevant report on the preparation of matrix-type biomimetic healing materials by blending SIS and PRP.

Method used

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  • Preparation method for biomimetic matrix type biological wound healing material
  • Preparation method for biomimetic matrix type biological wound healing material
  • Preparation method for biomimetic matrix type biological wound healing material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The steps of the preparation method of the bionic matrix type biological healing material of the present invention are as follows:

[0032] Step 1: Select porcine small intestines with traceable sources that meet inspection and quarantine requirements, scrape off redundant tissues such as muscle and serosa, rinse with deionized water, and soak in EDTA (100mmol / L) / NaOH (10mmol / L) solution for 16 hours , rinsed with deionized water and placed in hydrochloric acid / PBS solution with a pH value of 3~4 for 6~8h, rinsed with deionized water to remove residual acid solution, soaked in phosphate buffer saline (PBS) for 16~18h, repeated in deionized water rinse. Soak in 0.1% peracetic acid / 20% ethanol solution for 8 hours, then wash in 0.05% sodium azide / PBS solution for 2 hours, and sterilize by γ-ray irradiation. Homogenize aseptically to prepare SIS hydrated gel.

[0033] Step 2: Prepare platelet-rich plasma (PRP) by secondary centrifugation: select a 50ml sterile needle tub...

Embodiment 2

[0040] The steps of the preparation method of the bionic matrix type biological healing material of the present invention are as follows:

[0041] The first step: with embodiment 1.

[0042] Second step: with embodiment 1.

[0043] The third step. Take the prepared SIS hydration gel 0.5g and PRP 0.25g, stir and mix.

[0044] Step 4: Add 0.2% Genipin / NaHCO to the mixture in Step 3 by aseptic operation at 4°C 3 Solution (pH value 10) 0.75ml, mix well, let stand for 1.5h, add 10% CaCl 2 Solution 3.5ml, mix well. Cross-linking at room temperature for 5.5h.

[0045] Step 5: Discard the residual solution, and rinse repeatedly with 10ml of sterile PBS solution several times.

[0046] Step 6: Put the above reaction product into a sterile mold, freeze and thaw repeatedly at -20°C for 3 times, and then freeze-dry and shape it to obtain a biomimetic matrix biocure material.

Embodiment 3

[0048] The steps of the preparation method of the bionic matrix type biological healing material of the present invention are as follows:

[0049] The first step: with embodiment 1.

[0050] Second step: with embodiment 1.

[0051] Step 3: Take the prepared SIS hydration gel 0.5g and PRP 0.5g, stir and mix.

[0052] Step 4: Add 0.25% alginate / acetic acid solution (pH 5) 0.5ml to the mixture in step 3 under aseptic operation at 4°C, mix well, let stand for 1 hour, add 10 %CaCl 2 Solution 4.5ml, mix well. Crosslink at room temperature for 5 h.

[0053] Step 5: Discard the residual solution, and rinse with 12.5ml sterile PBS several times in small quantities.

[0054] Step 6: Put the above-mentioned reaction product into a sterile mold, freeze and thaw repeatedly at -20°C for 3 times, and then freeze-dry and shape it to obtain a biomimetic matrix biocure material.

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Abstract

The invention provides a method for preparing a new type of bionic matrix type biohealing material. The invention uses small intestinal submucosa with a three-dimensional scaffold structure and platelet-rich plasma containing a specific proportion of bioactive factors to compound, and is modified by a specific process. Finally, a new material with a three-dimensional biomimetic structure containing biologically induced factors is formed. The biomimetic matrix biohealing material made by the invention can not only promote healing but also promote physiological and inhibit pathological repair, and improve the regenerative repair efficiency and repair quality of damaged tissues. Moreover, this new type of bio-healing material can be used for the repair of various soft tissues, membranous tissues or cavity walls of the human body, and the filling of cavities, and can be used as an ideal extracellular matrix biomimetic and cell scaffold material for tissue and organ repair. Wound repair, promotion of wound healing and anti-adhesion, etc.

Description

technical field [0001] The invention relates to a method for preparing a medical bionic material, in particular to a method for preparing a composite and biomimetic biomimetic matrix type biological healing material through a specific crosslinking method. Background technique [0002] The regeneration and repair of tissue defects caused by factors such as trauma, infection, tumor, functional atrophy, and surgery has always been one of the main problems in clinical treatment. Solving the above-mentioned problems through alternative tissue repair with biological healing materials is a current research hotspot, and it is also the focus of general attention of scholars at home and abroad. The structure and performance of bio-healing materials currently available clinically are significantly different from those of body tissues. After implantation, they stimulate each other with the body to produce various irritating reactions, which seriously affects the quality of clinical trea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36
Inventor 张长青位晓娟顾其胜谢宗平程相国陈圣宝
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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