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Recombinant attenuated salmonella vaccine and pharmaceutical composition for treating solid tumors and application thereof

A Salmonella, solid tumor technology, applied in drug combination, gene therapy, anti-tumor drugs and other directions, can solve the problem of tumor gene therapy can not be anti-tumor gene delivery, etc., to achieve the effect of convenient medication route, high safety, and enhanced killing effect

Inactive Publication Date: 2012-07-04
LANZHOU GENERAL HOSPITAL OF LANZHOU MILITARY REGION PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The main obstacle of tumor gene therapy is the inability to specifically deliver anti-tumor genes into tumor tissue

Method used

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  • Recombinant attenuated salmonella vaccine and pharmaceutical composition for treating solid tumors and application thereof
  • Recombinant attenuated salmonella vaccine and pharmaceutical composition for treating solid tumors and application thereof
  • Recombinant attenuated salmonella vaccine and pharmaceutical composition for treating solid tumors and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] (1) Recombinant plasmid carrying interleukin 2 and hepatocyte growth factor antagonist NK4 gene Construction and Preparation of pCMV-IL-2-IRES-NK4 and Recombinant Attenuated Salmonella TPIN

[0071] (A) Cloning of IL-2 cDNA

[0072] Human interleukin 2 (interleukin- 2. Primers were designed for IL-2) cDNA sequence, an XhoI restriction site was introduced into the forward primer, and an MLuI restriction site was introduced into the reverse primer. The oligonucleotide sequence of the forward primer is 5-gacctcgagatgtacaggatgcaactc-3, and the oligonucleotide sequence of the reverse primer is 5-cgaacgcgttcacagtgttgagatgatgc-3, using conventional molecular biology techniques, from a human placenta cDNA library (purchased from Clontech Company, The human interleukin 2 (IL-2) gene was cloned by polymerase chain reaction (PCR) in USA). The PCR parameters were: 94°C pre-denaturation for 5 min, 94°C for 30 s, 48°C for 30 s, 72°C for 1 min, 30 cycles, and 72°C for 10 min. ...

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Abstract

The invention provides a recombinant attenuated salmonella vaccine and pharmaceutical composition for treating solid tumors and application thereof. The invention relates to application of cytokines in the prevention and the targeted treatment of the solid tumors and use of genes with interleukin2-combined hepatocyte growth factor antagonists in the preparation of drugs for the prevention and the targeted treatment of the solid tumors. The invention further relates to a gene-based pharmaceutical composition containing the interleukin2-combined hepatocyte growth factor antagonists and a method for applying the interleukin2-combined hepatocyte growth factor antagonists to a needed subject for the prevention and the targeted treatment of the solid tumors. According to the recombinant attenuated salmonella vaccine and the pharmaceutical composition for treating the solid tumors and the application thereof, the recombinant attenuated salmonella vaccine and the pharmaceutical composition can be beneficial to the prevention and the targeted treatment of various solid tumors.

Description

technical field [0001] The present invention relates to the application of the attenuated Salmonella oral vaccine for the prevention and targeted treatment of solid tumors carrying human interleukin 2 and the eukaryotic co-expression plasmid vector of the human hepatocyte growth factor antagonist NK4 gene at the same time, in particular to the application of simultaneously carrying human The application of the recombinant attenuated Salmonella vaccine of interleukin 2 and human hepatocyte growth factor antagonist double gene in the prevention and treatment of early, middle and late solid tumor diseases. Background technique [0002] Malignant tumor is one of the most important diseases that endanger human health and life. The latest epidemiological survey results show that there are more than 3 million cancer patients in my country, which continues to increase at a rate of 3% per year. Surgery, radiotherapy and chemotherapy are the main methods for the treatment of malignan...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K35/74A61K9/48A61P35/00A61K39/00
Inventor 哈小琴张尚弟尹强张俊惠玲王美亮张红宾贾庆华王晓辉昌业伟
Owner LANZHOU GENERAL HOSPITAL OF LANZHOU MILITARY REGION PLA
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