Method for detecting glycoprotein

Abstract

The invention discloses a method for detecting glycoprotein. The method comprises the following steps of: marking the glycoprotein by using zinc oxide quantum dots; meanwhile, immobilizing an agglutinin layer on a chip in an electrolytic cell; specifically binding the glycoprotein marked by the zinc oxide quantum dots with agglutinin on the chip in a series of glycoprotein solutions to be determined of known concentrations; connecting the zinc oxide quantum dots marked on the glycoprotein to the chip; and determining the agglutinin by using dissolving voltammetry of marking the glycoprotein with the zinc oxide quantum dots. Since the marked glycoprotein has different binding capacities to the glycoprotein to be determined and the agglutinin, the glycoprotein marked on the chip is substituted by the glycoprotein to be determined of different concentrations, and the concentration of zinc ions which are remained on the chip and dissolved out is determined by using the dissolving voltammetry to establish relation between the concentration of the zinc ions and the concentration of the glycoprotein so as to determine the concentration of the glycoprotein to be determined.

Description

Technical field [0001] The present invention belongs to the field of pharmaceutical and biotechnology. Specifically, the invention describes the preparation and detection method of a glucose detection chip. Background technique [0002] The glycoprotein in biomolecules plays an important role in many life activities and metabolic regulation. The changes in some protein surface glycosylation usually and indicate that some diseases, especially cancer -related glycoprotein.The importance of glycoprotein in the subject of life has also led to the rapid development of glycin group.The main task of this discipline is to analyze the relevant sugar clan of some sugar protein.Traditional analysis methods include mass spectrometers and chromatography.Although these methods are already mature, there are some problems, such as the equipment itself is expensive, the analysis is time -consuming, and professionals need to operate.As a result, these technologies have hindered the popularization ...

AI Technical Summary

Problems solved by technology

Although these methods are very mature, there are still some problems, such as expensive equipment itself, time-consuming analysis, and the need for professional operation

Therefore, it also hinders the popularization of these techniques in glycoprotein detection.

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