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Method for detecting NO (nitric oxide) content in exhaled gas of human body

A technology for exhaling gas and detecting human body, which is applied in measurement devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of reducing the clinical value of NO detection, low sensitivity, time-consuming and labor-intensive, etc., and achieves high sensitivity and operation. Convenience and simplicity, the effect of less reagents

Inactive Publication Date: 2012-06-20
EAST CHINA UNIV OF TECH
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Problems solved by technology

In short, these methods require certain pretreatment and various chemical and derivatization reactions before they can be detected. Not only the sensitivity is not high, but the process is cumbersome, time-consuming and labor-intensive, which greatly reduces the clinical value of NO detection.

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  • Method for detecting NO (nitric oxide) content in exhaled gas of human body
  • Method for detecting NO (nitric oxide) content in exhaled gas of human body

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Embodiment Construction

[0012] A method for detecting NO content in exhaled air from a human body, comprising the following steps:

[0013] 1. Use 3-oxo-2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxygen (PTIO) as the solute and methanol as the solvent to prepare a 50ppb PTIO reagent solution for later use;

[0014] 2. Debug the electrospray extraction ionization source. The angle α between the extraction agent introduction pipe 1 and the horizontal plane is 145°. The extraction agent carrier gas pipe 3 is set on the extraction agent introduction pipe 1. A 3.5kv high-voltage electrode is added to the extraction agent introduction pipe 1. 5. Spray auxiliary gas N 2 , the air pressure is 1.6MPa to pass in the extractant carrier gas pipe 3, the extractant methanol is passed into the extractant introduction pipe 1 with a flow rate of 6 μL / min, the extraction agent carrier gas pipe 3, and the extraction agent introduction pipe 1 are fixed by the upper bracket 6; The angle β between the introduction pipe 2 ...

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Abstract

The invention discloses a method for detecting NO (nitric oxide) content in exhaled gas of a human body, wherein PTIO (3-oxo-2-phenyl-4,4,5,5-tetramethyl imidazoline-1-oxygen) is utilized as an oxidizing agent of the NO, and the content of the NO in the exhaled gas of the human body can be directly and quickly detected by adopting an electrospray ionization extraction mass spectrometry method. The method disclosed by the invention has strong specificity and high sensitivity without pre-treatment on a sample; the PTIO has better stability and reaction sensitivity, and can selectively react with the NO. In addition, the method is integrated with sample collection and treatment, so that the consumed dose is small, the operation is convenient and simple and the detection can be finished in a short time. The method has the advantages of high mass spectrum sensitivity and good stability, breaks through the disadvantage of a traditional method, and realizes fast and sensitive monitoring of the NO in the exhaled gas of the human body.

Description

technical field [0001] The invention relates to a method for detecting the NO content in the exhaled gas of a human body. Background technique [0002] At present, there are commercial NO detectors for detecting the NO content in exhaled air. This kind of special detection equipment needs to consume standard gas, and the operation is complicated and time-consuming. Other traditional detection methods for NO in exhaled air are chemiluminescence, which needs to excite NO into excited state NO in the presence of ozone 2 ·, NO 2 Re-emission of light to generate stable NO 2 , to detect NO by detecting the light it emits. This method not only requires sample collection and pretreatment, but also requires ozone assistance. Spectrophotometry also needs to collect and condense the sample, which mainly uses NO to be easily oxidized into NO 2 - , NO under acidic conditions 2 - Promote the reaction Griess to generate diazo compounds, and then detect diazo compounds by spectrophot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64
Inventor 陈焕文丁健桦张燕张兴磊肖赛金欧阳永中黄艳红
Owner EAST CHINA UNIV OF TECH
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