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Solid fermentation method of lactarius deliciosus mycelium and application of solid fermentation extractive thereof

A technology of solid fermentation and mycelium, applied in the direction of application, fertilizer mixture, fertilization device, etc., to achieve the effect of wide source, low price and easy promotion

Inactive Publication Date: 2012-06-20
肖兵南
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to relevant research, solid fermentation of mycelia can overcome the above problems, but the research on solid fermentation of Lactari pine mushroom mycelium is rarely reported; in addition, the research on fungal active components is mostly limited to polysaccharides, while the research on peptides has not been reported.

Method used

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  • Solid fermentation method of lactarius deliciosus mycelium and application of solid fermentation extractive thereof
  • Solid fermentation method of lactarius deliciosus mycelium and application of solid fermentation extractive thereof
  • Solid fermentation method of lactarius deliciosus mycelium and application of solid fermentation extractive thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Effects of Different Proportions of Medium on the Production of Intracellular Polysaccharides and Peptides in the Mycelia of Lactarius pine

[0034] 1. Materials

[0035] Pine milk mushroom strains: preserved by Hunan Minkang Biotechnology Research Institute. There are multiple methods for obtaining the pine milk mushroom bacterial classification, which are introduced in the prior art. The present invention has nothing to do with the pine milk mushroom strain itself.

[0036] The solid medium substrates generally used for pine milk mushrooms are: oil bran powder, bran, rice bran, straw powder, DDGS, rice flour, corn husk, corn flour, puffed corn, soybean meal, rapeseed meal, cotton meal, etc., according to different proportions preparation.

[0037]PDA nutrient solution: 1% corn steep liquor, 0.15% ammonium nitrate, 0.01% magnesium sulfate heptahydrate, 0.4% sodium carboxymethylcellulose, 1.5% glucose, distilled water to 100ml, pH6.5.

[0038] 2. Method

[0039] Th...

Embodiment 2

[0047] Effects of Different Conditions of Solid Fermentation of Lactobacillus pine mushroom mycelium on Polysaccharide Polypeptide Production

[0048] 1. Materials

[0049] Pine milk mushroom strains: preserved by Hunan Minkang Biotechnology Research Institute.

[0050] Pine milk mushroom mycelium solid fermentation medium: by bran: corn bran: soybean meal: puffed corn=4:3:1:1 (mass ratio)

[0051] PDA nutrient solution: 1% corn steep liquor, 0.15% ammonium nitrate, 0.01% magnesium sulfate heptahydrate, 0.4% sodium carboxymethylcellulose, 1.5% glucose, distilled water to 100ml, pH6.5.

[0052] Different ratios of medium.

[0053] 2. Method

[0054] The bacterial classification on the slant medium is inoculated on the plate of PDA solid medium to activate once, then select the good mycelium of 2cm size and grow in the Erlenmeyer flask that PDA liquid medium is housed, on the constant temperature shaker (rotating speed 150rpm ) at 26° C. for 24 h to obtain the bacterial susp...

Embodiment 3

[0076] Effects of adding 11 kinds of plant Chinese herbal medicines on the production of polysaccharides and polypeptides by solid fermentation of Lactarius pine mushroom mycelium

[0077] The best culture medium obtained in Example 1 was fermented under the best culture conditions obtained in Example 2, and 11 kinds of plant Chinese herbal medicines were added during the fermentation process, and the addition of plant Chinese herbal medicines was 10% of the total mass of the medium. %. Through daily observation, the growth rate of the mycelia of Pine Lactarius slowed down, and the yield of polysaccharides did not change significantly, while the yield of polypeptides increased significantly, especially when adding Chinese yam, Ophiopogon japonicus, white spoon, and Gynostemma pentaphyllum. obviously increase. The determination results of its polysaccharide polypeptide are shown in Table 7.

[0078] Table 7 Effects of botanical Chinese herbal medicines on the production of po...

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Abstract

The invention relates to the technical field of solid fermentation, in particular to a solid fermentation method of lactarius deliciosus mycelium and an application of solid fermentation extractive thereof. The solid fermentation method of lactarius deliciosus mycelium comprises the steps of: performing solid fermentation on the lactarius deliciosus mycelium by a culture medium of the invention under certain culture conditions, wherein the certain culture conditions are as follows: the carbon-nitrogen ratio of the culture medium is 1:5 to 1:8, the temperature is 18-37 DEG C, the pH is 5-8, the day age of strain is 24-120 h, the inoculation amount is 10-30%, and the fermentation period is 2-7 d. The product obtained after the solid fermentation procedure of the solid fermentation method is extracted by such methods as ultramicro pulverization, enzyme treatment and microwave or ultrasonic, and the like so as to obtain an extractive containing polysaccharide and polypeptide. The extractive can be used as a food additive to be applied in the healthcare field and can be also used as a feed additive to be applied in the animal feed field. According to the invention, a proper dosage of plant Chinese medicinal herb additives can be added in the culture medium to improve the yield of the mycelium and the intracellular and extracellular polysaccharide and polypeptide so as to reinforce the pharmacological activity of the extractive.

Description

technical field [0001] The invention relates to the technical field of solid fermentation, in particular to the solid fermentation of wild fungus mycelium and the application of its solid fermentation extract in animal and human health care, especially the solid culture of pineapple mycelium and the extraction of its culture Preparation of polysaccharide polypeptide additives for animal and human health care applications. Background technique [0002] According to reports, the polysaccharides of many medicinal and food (medicine) fungi have the functions of improving immunity, anti-oxidation, antibacterial, anti-virus and anti-tumor, and are an important direction for the development of animal and human health products. A number of products such as lentinan were launched. The source of its raw materials is the fruiting body taken from the wild or cultivated artificially, and the second is the artificially fermented mycelium. Liquid submerged fermentation is an important me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00C05G3/00
Inventor 肖兵南
Owner 肖兵南
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