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Tea saponine nanocapsule, preparation method and application thereof

A technology of tea saponin and nanocapsules, which is applied in the field of medicine to achieve the effects of enhanced stability, good activity and simple preparation process

Inactive Publication Date: 2012-06-20
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tea saponin has not been made into a microencapsulated preparation

Method used

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  • Tea saponine nanocapsule, preparation method and application thereof
  • Tea saponine nanocapsule, preparation method and application thereof
  • Tea saponine nanocapsule, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Dissolve 100 g of tea saponin in 1900 g of water, add an equal volume of n-butanol to extract once, add 2 g of sodium hydroxide, and reflux at 80 ° C for 5 hours. Recover n-butanol under reduced pressure, dissolve the precipitate with methanol, and crystallize and dry to obtain 65 g of tea saponin; 10 g of polymethyl methacrylate and 90 g of ethanol are made into a capsule material solution, and 0.5 g of monoglyceride and 10 g of tea saponin are added. Stir and emulsify for 0.5 hour to obtain 100 ml of emulsion. Slowly add the above-mentioned emulsion to 5 times the volume (500ml) of pH 2 aqueous solution containing 0.1% (0.5ml) Tween 20, stir for 1 hour, pass through a microporous membrane, and let stand to separate layers; the precipitate is dried to obtain tea Saponin nanocapsules 20g.

Embodiment 2

[0027] Dissolve 100 g of tea saponin in 900 g of water, add an equal volume of n-butanol to extract twice, add 3 g of sodium hydroxide, and reflux at 90° C. for 3 hours. Recover n-butanol under reduced pressure, dissolve the precipitate with ethanol, crystallize and dry to obtain 75g of tea saponin; 5g of polyalkylisocyanate and 95g of acetone are made into a capsule material solution, add 5g of sucrose fatty acid ester, 3g of tea saponin, stir and emulsify After 1 hour, 100 ml of emulsion was obtained. Slowly add the emulsion to 10 times the volume (1000ml) of an aqueous solution containing 0.5% (5ml) poloxamer at pH 3, stir for 2 hours, pass through a microporous membrane, and leave to separate layers; the precipitate is dried to obtain tea saponin Nanocapsules 8g.

Embodiment 3

[0029] Dissolve 60 g of tea saponin in 340 g of water, add an equal volume of n-butanol to extract 3 times, add 2 g of sodium hydroxide, and reflux at 100° C. for 4 hours. Recover n-butanol under reduced pressure, dissolve the precipitate with acetone, and crystallize and dry to obtain 38g of tea saponin; 3g of acrylic resin is made into a capsule material solution with 97g of acetone, add 1g of monoglyceride and 2g of tea saponin, stir and emulsify for 1.5 hours, 100 ml of emulsion are obtained. Slowly add the emulsion to 15 times the volume (1500ml) of an aqueous solution containing 1% (15ml) poloxamer at pH 5, stir for 3 hours, pass through a microporous membrane, and leave to separate layers; the precipitate is dried to obtain tea saponin Nanocapsules 5g.

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Abstract

The invention discloses a tea saponine nanocapsule, a preparation method and application thereof, i.e. tea saponin is dissolved with water and is extracted with n-butyl alcohol, 1-5% of sodium hydroxide is added, the mixture is refluxed for 2-5h at 80-100 DEG C, and is decompressed to recycle the n-butyl alcohol, a solvent is used to dissolve the sediment, then crystallization and drying are carried out, so the tea saponine is obtained; and a capsule material is produced into 1-10% of capsule material solution with the solvent, 0.5-5% of emulsifier and 1-10% of tea saponine are added to be stirred and emulsified for 0.5-2h, the emulsion is slowly added into aqueous solution with the pH of 2-6 and 0.1-1% of emulsifier to stir for 1-3h, the mixture passes a millipore filter and is stood for layering, the sediment is dried, i.e. the tea saponine nanocapsule is obtained. The nanocapsule can be produced into sterilizing sterilized, anti-inflammatory and immunity supplement preparation. The nanocapsule improves the stability of the tea saponine, and obviously improves the sterilizing, anti-inflammatory and immunity supplement effects.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method of tea saponin nanocapsule and its application as a bactericidal, anti-inflammatory and immune-enhancing drug. Background technique [0002] Tea saponin is a major pentacyclic triterpenoid compound in tea seed or camellia oleifera. It has various biological activities such as antibacterial, anti-inflammatory and enhancing immune function. my country is a country with a high yield of camellia oleifera, and the content of saponin in camellia oleifera seeds accounts for 12%. At present, it is used as a surfactant after extraction, but it has not been effectively used in medicine. [0003] Domestically produced tea saponin mostly uses camellia oleifera seeds as raw materials. After degreasing the camellia oleifera seeds, extracting them with methanol water or ethanol water and drying them, after technical improvement, tea saponin can be directly copolymerized with styren...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K9/19A61K9/20A61K9/48A61K36/82A61K31/704B01J13/02A61P29/00A61P31/04A61P37/04
Inventor 叶勇罗月婷
Owner SOUTH CHINA UNIV OF TECH
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