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Preparation method and use of soluble squid ink melanin

A technology of melanin and squid ink is applied in the application of antioxidant activity, in the field of preparing soluble melanin, and achieves the effects of high application potential and economy, simple preparation process and high content

Inactive Publication Date: 2012-06-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation method of obtaining soluble melanin from squid ink by ultrasonic treatment in lye has not been reported yet

Method used

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  • Preparation method and use of soluble squid ink melanin
  • Preparation method and use of soluble squid ink melanin
  • Preparation method and use of soluble squid ink melanin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1, a kind of preparation method of soluble squid ink melanin, carries out following steps successively:

[0049] 1) Thaw the frozen squid ink sac at room temperature (fresh squid ink sac does not need this thawing step), squeeze and remove the skin membrane and internal reticular membrane on the surface of the ink sac to obtain the ink suspension. Carry out the following washing work successively with the ink suspension:

[0050] A, the ink suspension 20g is soaked overnight at 0-4° C. with 2 volume times of ice water (that is, ice water is 2 times the volume of the ink suspension), centrifuged at 8000r / min for 20 minutes, and the supernatant ( The supernatant contains impurities);

[0051] B. Add twice the volume of ice water to the resulting precipitate (that is, ice water is twice the volume of the precipitate), centrifuge at 8000r / min for 20 minutes, and remove the supernatant;

[0052] C. Repeat the above step B 2 to 3 times;

[0053] D. Add 20 times ...

Embodiment 2

[0069] Embodiment 2, soluble melanin component is to superoxide radical activity inhibitory action:

[0070] 9 kinds of components of embodiment 1 gained are carried out as follows respectively:

[0071] Use PBS buffer (0.05mol / L, pH 7.8) to prepare solutions with concentrations of 0, 50, 100, 150, 200, 300, 350, and 400ug / ml, add 400uL each to a small test tube, and then add pyrogallol (0.625umol / L) 50uL, quickly add 550uL luminol (1mmol / L, with 0.1mol / L NaCO 3 Preparation), pull the cover, and start measuring the luminol chemiluminescence value C. Three parallel experiments were performed for each set of experiments. Inhibition rate (%)=(C0-C) / C0×100% (C0 is the luminescence value of the blank without adding antioxidant, and C is the luminescence value after adding antioxidant).

[0072] According to the relationship curve between concentration and inhibition rate ( figure 1 ), the concentration of the sample when the inhibition rate reaches 50% is IC 50 The results are...

Embodiment 3

[0076] Example 3, soluble melanin components inhibit the activity of hydroxyl radicals:

[0077] 9 kinds of components of embodiment 1 gained are carried out as follows respectively:

[0078] Use PBS buffer (0.05mol / L, pH 7.8) to prepare solutions with concentrations of 0, 50, 100, 150, 200, 250, 350, 450, and 550ug / ml, add 550uL to each small test tube, and then add 0.2mL Ascorbic acid (2mmol / L), 0.4mL CuSO 4 (2mmol / L), 50uL luminol (0.1mmol / L, with 0.1mol / L NaCO 3 Preparation), 0.2mL yeast suspension (75mg / mL), mix well and incubate for 30min. Then add 0.6mL H 2 o 2 (6.8 mmol / L) to initiate the reaction. Pull up the cover and start to measure the luminol chemiluminescence value C. Inhibition rate (%)=(C0-C) / C0×100% (C0 is the luminescence value of the blank without adding antioxidant, and C is the luminescence value after adding antioxidant).

[0079] According to the relationship curve between concentration and inhibition rate ( figure 2 ) can get the concentration...

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Abstract

The invention discloses a preparation method of soluble squid ink melanin. The preparation method comprises the following steps of: 1) washing ink suspension in the ink sac of a squid with ice water at the temperature of 0-4 DEG C, centrifugating and then removing supernatant fluid to obtain a precipitate; 2) performing vacuum freeze-drying on the precipitate obtained in the step 1) to obtain an insoluble melanin crude product; 3) dissolving the insoluble melanin crude product in alkali liquor and performing ultrasonic processing for 30-90 minutes, wherein the material-liquid ratio of the insoluble melanin crude product to the alkali liquor is 1g of the insoluble melanin crude product / 20-80ml of the alkali liquor, and the alkali liquor is an NaOH solution in the molar concentration of 0.1-1.0mol / L; and 4) centrifugating the product obtained in the step 3), taking the supernatant fluid and performing vacuum freeze-drying to obtain the soluble squid ink melanin. The invention further provides a use of the soluble squid ink melanin as an antioxidant.

Description

technical field [0001] The invention relates to a method for preparing melanin, in particular to a method for preparing soluble melanin from squid ink, and the application of the antioxidant activity thereof. Background technique [0002] Squid is a common name for squid and squid among cephalopod marine animals. North Pacific squid is one of the most important squid processing species, the annual processing volume is between 150,000 and 300,000 tons, and its ink sac accounts for about 1.3% of the whole body weight. A large amount of waste generated after squid processing, especially the ink sac, has not been well utilized, and has also caused great pollution to the environment. At the same time, squid ink contains a variety of biologically active substances such as polypeptide fragments, acidic polysaccharides, tyrosinase, etc. Therefore, the extraction of melanin from squid ink not only improves the comprehensive utilization rate of waste in the squid processing process,...

Claims

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Application Information

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IPC IPC(8): A23L1/327A23L1/30A61K35/56A61P39/06A23L17/20A61K35/618
Inventor 陈士国郭欣叶兴乾薛长湖
Owner ZHEJIANG UNIV
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