Production method for hepatitis A virus for vaccine production

A technology for hepatitis A virus and vaccine production, applied in the biological field, can solve the problems of high production intensity, low production level, complicated operation, etc., and achieve the effects of improving yield and quality stability, low production intensity, and ensuring production quality

Inactive Publication Date: 2012-06-13
CHENGDU KANGHUA BIOLOGICAL PROD
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main production method adopted in China is to adopt static low-density culture methods such as rotating bottle and cell factory to cultivate virus antigen, and then prepare hepatitis A vaccine through purification and other processes. This production process has low production level and insufficient stability of process parameters. At the same time, the operation is complicated, the production intensity is high, and the pollution is easy to occur.
At the same time, due to the small batch size of the traditional production process, there are large differences between batches of vaccine products, and the stability of product quality is not good enough.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A production method of hepatitis A virus for vaccine production, comprising the following steps in sequence:

[0027] Step 1: Recovery and subculture expansion of cells: recovery of cells, and culturing cells in a granular solid microsphere carrier bioreactor;

[0028] Step 2: inoculate hepatitis A virus seeds: when the cell concentration is 10 6 Individuals / ml, the culture medium was discarded, and the HM175 strain of hepatitis A virus was mixed and inoculated according to the ratio of 0.001 MOI;

[0029] Step 3: maintaining and culturing the cells infected with the HM175 strain of hepatitis A virus: maintaining and culturing the cells infected with the HM175 strain of hepatitis A virus with MEM maintenance solution, replacing the MEM maintenance solution every day, and maintaining the culture for 14 days;

[0030] Step 4: Detect the replication of virus infection: From the 14th day, samples were taken every day, and the virus antigen in the cells was measured by immu...

Embodiment 2

[0036] A production method of hepatitis A virus for vaccine production, comprising the following steps in turn:

[0037] Step 1: recovery and passage expansion of cells: recovery of cells, and cultivation of cells in a porous microsphere carrier bioreactor;

[0038] Step 2: inoculate hepatitis A virus seeds: when the cell concentration is 10 7 Individuals / ml, the culture medium was discarded, and the HM175 strain of hepatitis A virus was mixed and inoculated according to the ratio of 0.01 MOI;

[0039] Step 3: maintaining and culturing cells infected with HM175 strain of hepatitis A virus: maintaining and culturing cells infected with HM175 strain of hepatitis A virus with MEM maintenance solution, replacing MEM maintenance solution every day, and maintaining the culture for 28 days;

[0040] Step 4: Detect the replication of virus infection: From the 14th day, samples were taken every day, and the virus antigen in the cells was measured by immunofluorescence method to detect...

Embodiment 3

[0046] A production method of hepatitis A virus for vaccine production, comprising the following steps in sequence:

[0047] Step 1: Recovery and subculture expansion of cells: recovery of cells, and culturing cells in a granular solid microsphere carrier bioreactor;

[0048] Step 2: inoculate hepatitis A virus seeds: when the cell concentration is 10 6 Individuals / ml, the culture medium was discarded, and the HM175 strain of hepatitis A virus was mixed and inoculated according to the ratio of 0.001 MOI;

[0049] Step 3: maintaining and culturing the cells infected with the HM175 strain of hepatitis A virus: maintaining and culturing the cells infected with the HM175 strain of hepatitis A virus with MEM maintenance solution, replacing the MEM maintenance solution every day, and maintaining the culture for 14 days;

[0050] Step 4: Detect the replication of virus infection: From the 14th day, samples were taken every day, and the virus antigen in the cells was measured by immu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of biotechnology, in particular to a production method for a hepatitis A virus for vaccine production. The production method comprises the following steps sequentially: reviving, passaging and amplifying a cell; vaccinating a hepatitis A virus seed; keeping culturing the cell infected with hepatitis A virus; detecting the reproduction condition of viral infection; stopping culturing, discarding the maintenance medium, and reaping the cultured infected cell; collecting cell suspension; and extracting and purifying the cell suspension. The production method has the advantages that the operation is simple; the production intensity is low; the high-density large-scale cultivation of stromal cells is realized; the yield and the quality stability of hepatitis A vaccine are improved; and pollution caused by manual operation in the conventional manufacturing technology is avoided.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a production method of hepatitis A virus. Background technique [0002] Hepatitis A, referred to as hepatitis A, is an infectious disease caused by hepatitis A virus. The transmission of the disease is mainly through the fecal-oral route. The hepatitis A virus is excreted with feces and can be transmitted orally through daily contact, water, food, etc. In developed countries, it is mainly transmitted through person-to-person contact, and it often occurs in developing countries and regions through contaminated water and food. The most prominent example is the two outbreaks of hepatitis A in Shanghai, both caused by eating uncooked cockles. In 1983, 20,000 people fell ill when it broke out for the first time in 1983. In the spring of 1988, there were nearly 300,000 patients, which caused serious losses to the national economy and people's health. [0003] At present, the main producti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12R1/93
Inventor 赵志鹏侯文礼
Owner CHENGDU KANGHUA BIOLOGICAL PROD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap