Production method for hepatitis A virus for vaccine production

Abstract

The invention relates to the field of biotechnology, in particular to a production method for a hepatitis A virus for vaccine production. The production method comprises the following steps sequentially: reviving, passaging and amplifying a cell; vaccinating a hepatitis A virus seed; keeping culturing the cell infected with hepatitis A virus; detecting the reproduction condition of viral infection; stopping culturing, discarding the maintenance medium, and reaping the cultured infected cell; collecting cell suspension; and extracting and purifying the cell suspension. The production method has the advantages that the operation is simple; the production intensity is low; the high-density large-scale cultivation of stromal cells is realized; the yield and the quality stability of hepatitis A vaccine are improved; and pollution caused by manual operation in the conventional manufacturing technology is avoided.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a production method of hepatitis A virus. Background technique [0002] Hepatitis A, referred to as hepatitis A, is an infectious disease caused by hepatitis A virus. The transmission of the disease is mainly through the fecal-oral route. The hepatitis A virus is excreted with feces and can be transmitted orally through daily contact, water, food, etc. In developed countries, it is mainly transmitted through person-to-person contact, and it often occurs in developing countries and regions through contaminated water and food. The most prominent example is the two outbreaks of hepatitis A in Shanghai, both caused by eating uncooked cockles. In 1983, 20,000 people fell ill when it broke out for the first time in 1983. In the spring of 1988, there were nearly 300,000 patients, which caused serious losses to the national economy and people's health. [0003] At present, the main producti...

AI Technical Summary

Problems solved by technology

[0003] At present, the main production method adopted in China is to adopt static low-density culture methods such as rotating bottle and cell factory to cultivate virus antigen, and then prepare hepatitis A vaccine through purification and other processes. This production process has low production level and insufficient stability of process parameters. At the same time, the operation is complicated, the production intensity is high, and the pollution is easy to occur.

At the same time, due to the small batch size of the traditional production process, there are large differences between batches of vaccine products, and the stability of product quality is not good enough.